Porphyrin biosynthesis in rhodopseudomonas palustris-II. Evidence on the existence of a factor regulating aminolevulinate synthetase activity

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1. 1. No changes in ALA-S activity were observed when different preparations of R. palustris were stored at 4°C for various periods of time. 2. 2. Mixing supernatants from pigmented and decoloured R. palustris cells, showed that the activity of ALA-S was several times higher than expected, suggestin...

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Autores principales: Viale, A.A., De Xifra, E.A.W., Del C. Batlle, A.M.
Formato: JOUR
Materias:
5 aminolevulinate synthase
porphyrin
aerobic metabolism
article
biosynthesis
kinetics
metabolism
molecular weight
photosynthesis
Rhodopseudomonas
5-Aminolevulinate Synthetase
Aerobiosis
Kinetics
Molecular Weight
Photosynthesis
Porphyrins
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0020711X_v12_n5-6_p729_Viale
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0020711X_v12_n5-6_p729_Viale
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spelling todo:paper_0020711X_v12_n5-6_p729_Viale2023-10-03T14:17:40Z Porphyrin biosynthesis in rhodopseudomonas palustris-II. Evidence on the existence of a factor regulating aminolevulinate synthetase activity Viale, A.A. De Xifra, E.A.W. Del C. Batlle, A.M. 5 aminolevulinate synthase porphyrin aerobic metabolism article biosynthesis kinetics metabolism molecular weight photosynthesis Rhodopseudomonas 5-Aminolevulinate Synthetase Aerobiosis Kinetics Molecular Weight Photosynthesis Porphyrins Rhodopseudomonas Support, Non-U.S. Gov't 1. 1. No changes in ALA-S activity were observed when different preparations of R. palustris were stored at 4°C for various periods of time. 2. 2. Mixing supernatants from pigmented and decoloured R. palustris cells, showed that the activity of ALA-S was several times higher than expected, suggesting the presence of an activator. 3. 3. Supernatants from photosynthetically and aerobically grown cells were heated and the effect of the protein-free supernatant was tested on both red and white supernatants. The heated supernatant from aerobic cells increased ALA-S when added to red and white preparations, but the heated red supernatant only activated red supernatant and had no action on the white cells enzyme. 4. 4. By gel filtration on Sephadex G-25 of cell free extracts from R. palustris either aerobically or anaerobically grown, a low molecular weight compound was separated, which added back to the homologeous enzyme enhanced its activity confirming the existence of one or two low-molecular weight and heat-stable factors which would act stimulating ALA-S activity. 5. 5. A scheme is proposed to explain the role of these factors on the control of ALA-S in R. palustris. © 1980. Fil:Viale, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0020711X_v12_n5-6_p729_Viale
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 5 aminolevulinate synthase
porphyrin
aerobic metabolism
article
biosynthesis
kinetics
metabolism
molecular weight
photosynthesis
Rhodopseudomonas
5-Aminolevulinate Synthetase
Aerobiosis
Kinetics
Molecular Weight
Photosynthesis
Porphyrins
Rhodopseudomonas
Support, Non-U.S. Gov't
spellingShingle 5 aminolevulinate synthase
porphyrin
aerobic metabolism
article
biosynthesis
kinetics
metabolism
molecular weight
photosynthesis
Rhodopseudomonas
5-Aminolevulinate Synthetase
Aerobiosis
Kinetics
Molecular Weight
Photosynthesis
Porphyrins
Rhodopseudomonas
Support, Non-U.S. Gov't
Viale, A.A.
De Xifra, E.A.W.
Del C. Batlle, A.M.
Porphyrin biosynthesis in rhodopseudomonas palustris-II. Evidence on the existence of a factor regulating aminolevulinate synthetase activity
topic_facet 5 aminolevulinate synthase
porphyrin
aerobic metabolism
article
biosynthesis
kinetics
metabolism
molecular weight
photosynthesis
Rhodopseudomonas
5-Aminolevulinate Synthetase
Aerobiosis
Kinetics
Molecular Weight
Photosynthesis
Porphyrins
Rhodopseudomonas
Support, Non-U.S. Gov't
description 1. 1. No changes in ALA-S activity were observed when different preparations of R. palustris were stored at 4°C for various periods of time. 2. 2. Mixing supernatants from pigmented and decoloured R. palustris cells, showed that the activity of ALA-S was several times higher than expected, suggesting the presence of an activator. 3. 3. Supernatants from photosynthetically and aerobically grown cells were heated and the effect of the protein-free supernatant was tested on both red and white supernatants. The heated supernatant from aerobic cells increased ALA-S when added to red and white preparations, but the heated red supernatant only activated red supernatant and had no action on the white cells enzyme. 4. 4. By gel filtration on Sephadex G-25 of cell free extracts from R. palustris either aerobically or anaerobically grown, a low molecular weight compound was separated, which added back to the homologeous enzyme enhanced its activity confirming the existence of one or two low-molecular weight and heat-stable factors which would act stimulating ALA-S activity. 5. 5. A scheme is proposed to explain the role of these factors on the control of ALA-S in R. palustris. © 1980.
format JOUR
author Viale, A.A.
De Xifra, E.A.W.
Del C. Batlle, A.M.
author_facet Viale, A.A.
De Xifra, E.A.W.
Del C. Batlle, A.M.
author_sort Viale, A.A.
title Porphyrin biosynthesis in rhodopseudomonas palustris-II. Evidence on the existence of a factor regulating aminolevulinate synthetase activity
title_short Porphyrin biosynthesis in rhodopseudomonas palustris-II. Evidence on the existence of a factor regulating aminolevulinate synthetase activity
title_full Porphyrin biosynthesis in rhodopseudomonas palustris-II. Evidence on the existence of a factor regulating aminolevulinate synthetase activity
title_fullStr Porphyrin biosynthesis in rhodopseudomonas palustris-II. Evidence on the existence of a factor regulating aminolevulinate synthetase activity
title_full_unstemmed Porphyrin biosynthesis in rhodopseudomonas palustris-II. Evidence on the existence of a factor regulating aminolevulinate synthetase activity
title_sort porphyrin biosynthesis in rhodopseudomonas palustris-ii. evidence on the existence of a factor regulating aminolevulinate synthetase activity
url http://hdl.handle.net/20.500.12110/paper_0020711X_v12_n5-6_p729_Viale
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