Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: Possible role of leptin
Placentas from gestational diabetes (GDM) suffer from structural and functional changes including overgrowth. That is why we aimed to study [ 3H]-leucine incorporation into protein in addition to translation signaling in placenta from GDM. Thus, we investigated the expression of leptin and leptin re...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00185043_v45_n6_p436_PerezPerez |
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todo:paper_00185043_v45_n6_p436_PerezPerez2023-10-03T14:15:38Z Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: Possible role of leptin Pérez-Pérez, A. Maymó, J.L. Gambino, Y.P. Guadix, P. Dueñas, J.L. Varone, C.L. Sánchez-Margalet, V. EIF4EBP1 gestational diabetes LEPR leptin mTOR translation trophoblastic cells initiation factor 4E leptin leptin receptor leucine mammalian target of rapamycin S6 kinase transcription factor E2F1 adult article clinical article controlled study explant female human immunoblotting placenta pregnancy diabetes mellitus pregnant woman priority journal protein expression protein phosphorylation protein synthesis signal transduction Adult Case-Control Studies Diabetes, Gestational Female Humans Leptin Placenta Pregnancy Receptors, Leptin Signal Transduction Young Adult Placentas from gestational diabetes (GDM) suffer from structural and functional changes including overgrowth. That is why we aimed to study [ 3H]-leucine incorporation into protein in addition to translation signaling in placenta from GDM. Thus, we investigated the expression of leptin and leptin receptor (LEPR), as well as the activation state of signaling proteins regulating protein synthesis, such as mTOR, S6 Kinase, EIF4E-BP1, EIF4E, and eEF2 by measuring protein phosphorylation by immunoblot. [ 3H]-Leucine incorporation into protein also was determined in trophoblastic placenta explants from GDM and control pregnancy. We found that leptin and LEPR expression are increased in placentas from GDM and the translation machinery activity as well as [3H]-leucine incorporation into protein were higher in placentas from GDM compared with placentas from control pregnancy. In conclusion, protein synthesis rate is increased in placenta from GDM patients, and this may be due, at least in part, by the activation of translation signaling. The increased expression of leptin and LEPR may contribute to these effects. These results may provide a possible mechanism for the previously observed increase in placenta growth in GDM. Fil:Maymó, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gambino, Y.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Varone, C.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00185043_v45_n6_p436_PerezPerez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
EIF4EBP1 gestational diabetes LEPR leptin mTOR translation trophoblastic cells initiation factor 4E leptin leptin receptor leucine mammalian target of rapamycin S6 kinase transcription factor E2F1 adult article clinical article controlled study explant female human immunoblotting placenta pregnancy diabetes mellitus pregnant woman priority journal protein expression protein phosphorylation protein synthesis signal transduction Adult Case-Control Studies Diabetes, Gestational Female Humans Leptin Placenta Pregnancy Receptors, Leptin Signal Transduction Young Adult |
| spellingShingle |
EIF4EBP1 gestational diabetes LEPR leptin mTOR translation trophoblastic cells initiation factor 4E leptin leptin receptor leucine mammalian target of rapamycin S6 kinase transcription factor E2F1 adult article clinical article controlled study explant female human immunoblotting placenta pregnancy diabetes mellitus pregnant woman priority journal protein expression protein phosphorylation protein synthesis signal transduction Adult Case-Control Studies Diabetes, Gestational Female Humans Leptin Placenta Pregnancy Receptors, Leptin Signal Transduction Young Adult Pérez-Pérez, A. Maymó, J.L. Gambino, Y.P. Guadix, P. Dueñas, J.L. Varone, C.L. Sánchez-Margalet, V. Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: Possible role of leptin |
| topic_facet |
EIF4EBP1 gestational diabetes LEPR leptin mTOR translation trophoblastic cells initiation factor 4E leptin leptin receptor leucine mammalian target of rapamycin S6 kinase transcription factor E2F1 adult article clinical article controlled study explant female human immunoblotting placenta pregnancy diabetes mellitus pregnant woman priority journal protein expression protein phosphorylation protein synthesis signal transduction Adult Case-Control Studies Diabetes, Gestational Female Humans Leptin Placenta Pregnancy Receptors, Leptin Signal Transduction Young Adult |
| description |
Placentas from gestational diabetes (GDM) suffer from structural and functional changes including overgrowth. That is why we aimed to study [ 3H]-leucine incorporation into protein in addition to translation signaling in placenta from GDM. Thus, we investigated the expression of leptin and leptin receptor (LEPR), as well as the activation state of signaling proteins regulating protein synthesis, such as mTOR, S6 Kinase, EIF4E-BP1, EIF4E, and eEF2 by measuring protein phosphorylation by immunoblot. [ 3H]-Leucine incorporation into protein also was determined in trophoblastic placenta explants from GDM and control pregnancy. We found that leptin and LEPR expression are increased in placentas from GDM and the translation machinery activity as well as [3H]-leucine incorporation into protein were higher in placentas from GDM compared with placentas from control pregnancy. In conclusion, protein synthesis rate is increased in placenta from GDM patients, and this may be due, at least in part, by the activation of translation signaling. The increased expression of leptin and LEPR may contribute to these effects. These results may provide a possible mechanism for the previously observed increase in placenta growth in GDM. |
| format |
JOUR |
| author |
Pérez-Pérez, A. Maymó, J.L. Gambino, Y.P. Guadix, P. Dueñas, J.L. Varone, C.L. Sánchez-Margalet, V. |
| author_facet |
Pérez-Pérez, A. Maymó, J.L. Gambino, Y.P. Guadix, P. Dueñas, J.L. Varone, C.L. Sánchez-Margalet, V. |
| author_sort |
Pérez-Pérez, A. |
| title |
Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: Possible role of leptin |
| title_short |
Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: Possible role of leptin |
| title_full |
Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: Possible role of leptin |
| title_fullStr |
Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: Possible role of leptin |
| title_full_unstemmed |
Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: Possible role of leptin |
| title_sort |
activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptin |
| url |
http://hdl.handle.net/20.500.12110/paper_00185043_v45_n6_p436_PerezPerez |
| work_keys_str_mv |
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1782031067594620928 |