Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)

Estrogens inhibit androgen production and this negative action on amphibian steroidogenesis could be related to the regulation of steroidogenic enzymes. Estrogens are also involved in the regulation of amphibian spermatogenesis by controlling testicular apoptosis and spermatogonial proliferation. Th...

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Autores principales:	Scaia, M.F., Volonteri, M.C., Czuchlej, S.C., Ceballos, N.R.
Formato:	JOUR
Materias:	Apoptosis Estradiol Proliferation Steroidogenesis Testes Toad 3 beta hydroxysteroid dehydrogenase isomerase 3beta hydroxy delta5 steroid dehydrogenase broxuridine cytochrome P450 17alpha hydroxylase carbon 17 20 lyase estradiol lyase unclassified drug 3 (or 17)-beta-hydroxysteroid dehydrogenase 3(or 17)beta hydroxysteroid dehydrogenase androgen aromatase hydroxysteroid dehydrogenase steroid 17alpha monooxygenase adult animal cell Anura apoptosis Article cell proliferation controlled study enzyme activity estradiol blood level fluorescence analysis male nonhuman priority journal protein expression Rhinella arenarum steroidogenesis testis TUNEL assay Western blotting animal Bufo arenarum cytology drug effects growth, development and aging metabolism physiology radioimmunoassay reproduction spermatogenesis 17-Hydroxysteroid Dehydrogenases 3-Hydroxysteroid Dehydrogenases Androgens Animals Aromatase Cell Proliferation Male Radioimmunoassay Reproduction Spermatogenesis Steroid 17-alpha-Hydroxylase Testis
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00166480_v221_n_p244_Scaia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00166480_v221_n_p244_Scaia
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spelling	todo:paper_00166480_v221_n_p244_Scaia2023-10-03T14:14:06Z Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura) Scaia, M.F. Volonteri, M.C. Czuchlej, S.C. Ceballos, N.R. Apoptosis Estradiol Proliferation Steroidogenesis Testes Toad 3 beta hydroxysteroid dehydrogenase isomerase 3beta hydroxy delta5 steroid dehydrogenase broxuridine cytochrome P450 17alpha hydroxylase carbon 17 20 lyase estradiol lyase unclassified drug 3 (or 17)-beta-hydroxysteroid dehydrogenase 3(or 17)beta hydroxysteroid dehydrogenase androgen aromatase estradiol hydroxysteroid dehydrogenase steroid 17alpha monooxygenase adult animal cell Anura apoptosis Article cell proliferation controlled study enzyme activity estradiol blood level fluorescence analysis male nonhuman priority journal protein expression Rhinella arenarum steroidogenesis testis TUNEL assay Western blotting animal apoptosis Bufo arenarum cell proliferation cytology drug effects growth, development and aging metabolism physiology radioimmunoassay reproduction spermatogenesis testis 17-Hydroxysteroid Dehydrogenases 3-Hydroxysteroid Dehydrogenases Androgens Animals Apoptosis Aromatase Bufo arenarum Cell Proliferation Estradiol Male Radioimmunoassay Reproduction Spermatogenesis Steroid 17-alpha-Hydroxylase Testis Estrogens inhibit androgen production and this negative action on amphibian steroidogenesis could be related to the regulation of steroidogenic enzymes. Estrogens are also involved in the regulation of amphibian spermatogenesis by controlling testicular apoptosis and spermatogonial proliferation. The Bidder's organ (BO) is a structure characteristic from the Bufonidae family and in adult males of Rhinella arenarum it is one of the main sources of plasma estradiol (E2). The purpose of this study is to analyze the effect of E2 on testicular steroidogenic enzymes, apoptosis and proliferation in the toad R. arenarum. For this purpose, testicular fragments were treated during 24h with or without 2 or 20nM of E2. After treatments, the activities of cytochrome P450 17α-hydroxylase-C17-20 lyase (CypP450c17) and 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD/I) were measured by the transformation of radioactive substrates into products, and CypP450c17 expression was determined by Western blot analysis. Apoptosis in testicular sections was detected with a commercial fluorescent kit based on TUNEL method, and proliferation was evaluated by BrdU incorporation. Results indicate that E2 has no effect on CypP450c17 protein levels or enzymatic activity, while it reduces 3β-HSD/I activity during the post reproductive season. Furthermore, although E2 has no effect on apoptosis during the pre and the post reproductive seasons, it stimulates testicular apoptosis during the reproductive season, mostly in spermatocytes. Finally, E2 has no effect on testicular proliferation all year long. Taken together, these results suggest that E2 is involved in the regulation of testicular steroidogenesis and spermatogenesis. © 2015 Elsevier Inc. Fil:Scaia, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Volonteri, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00166480_v221_n_p244_Scaia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Apoptosis Estradiol Proliferation Steroidogenesis Testes Toad 3 beta hydroxysteroid dehydrogenase isomerase 3beta hydroxy delta5 steroid dehydrogenase broxuridine cytochrome P450 17alpha hydroxylase carbon 17 20 lyase estradiol lyase unclassified drug 3 (or 17)-beta-hydroxysteroid dehydrogenase 3(or 17)beta hydroxysteroid dehydrogenase androgen aromatase estradiol hydroxysteroid dehydrogenase steroid 17alpha monooxygenase adult animal cell Anura apoptosis Article cell proliferation controlled study enzyme activity estradiol blood level fluorescence analysis male nonhuman priority journal protein expression Rhinella arenarum steroidogenesis testis TUNEL assay Western blotting animal apoptosis Bufo arenarum cell proliferation cytology drug effects growth, development and aging metabolism physiology radioimmunoassay reproduction spermatogenesis testis 17-Hydroxysteroid Dehydrogenases 3-Hydroxysteroid Dehydrogenases Androgens Animals Apoptosis Aromatase Bufo arenarum Cell Proliferation Estradiol Male Radioimmunoassay Reproduction Spermatogenesis Steroid 17-alpha-Hydroxylase Testis
spellingShingle	Apoptosis Estradiol Proliferation Steroidogenesis Testes Toad 3 beta hydroxysteroid dehydrogenase isomerase 3beta hydroxy delta5 steroid dehydrogenase broxuridine cytochrome P450 17alpha hydroxylase carbon 17 20 lyase estradiol lyase unclassified drug 3 (or 17)-beta-hydroxysteroid dehydrogenase 3(or 17)beta hydroxysteroid dehydrogenase androgen aromatase estradiol hydroxysteroid dehydrogenase steroid 17alpha monooxygenase adult animal cell Anura apoptosis Article cell proliferation controlled study enzyme activity estradiol blood level fluorescence analysis male nonhuman priority journal protein expression Rhinella arenarum steroidogenesis testis TUNEL assay Western blotting animal apoptosis Bufo arenarum cell proliferation cytology drug effects growth, development and aging metabolism physiology radioimmunoassay reproduction spermatogenesis testis 17-Hydroxysteroid Dehydrogenases 3-Hydroxysteroid Dehydrogenases Androgens Animals Apoptosis Aromatase Bufo arenarum Cell Proliferation Estradiol Male Radioimmunoassay Reproduction Spermatogenesis Steroid 17-alpha-Hydroxylase Testis Scaia, M.F. Volonteri, M.C. Czuchlej, S.C. Ceballos, N.R. Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)
topic_facet	Apoptosis Estradiol Proliferation Steroidogenesis Testes Toad 3 beta hydroxysteroid dehydrogenase isomerase 3beta hydroxy delta5 steroid dehydrogenase broxuridine cytochrome P450 17alpha hydroxylase carbon 17 20 lyase estradiol lyase unclassified drug 3 (or 17)-beta-hydroxysteroid dehydrogenase 3(or 17)beta hydroxysteroid dehydrogenase androgen aromatase estradiol hydroxysteroid dehydrogenase steroid 17alpha monooxygenase adult animal cell Anura apoptosis Article cell proliferation controlled study enzyme activity estradiol blood level fluorescence analysis male nonhuman priority journal protein expression Rhinella arenarum steroidogenesis testis TUNEL assay Western blotting animal apoptosis Bufo arenarum cell proliferation cytology drug effects growth, development and aging metabolism physiology radioimmunoassay reproduction spermatogenesis testis 17-Hydroxysteroid Dehydrogenases 3-Hydroxysteroid Dehydrogenases Androgens Animals Apoptosis Aromatase Bufo arenarum Cell Proliferation Estradiol Male Radioimmunoassay Reproduction Spermatogenesis Steroid 17-alpha-Hydroxylase Testis
description	Estrogens inhibit androgen production and this negative action on amphibian steroidogenesis could be related to the regulation of steroidogenic enzymes. Estrogens are also involved in the regulation of amphibian spermatogenesis by controlling testicular apoptosis and spermatogonial proliferation. The Bidder's organ (BO) is a structure characteristic from the Bufonidae family and in adult males of Rhinella arenarum it is one of the main sources of plasma estradiol (E2). The purpose of this study is to analyze the effect of E2 on testicular steroidogenic enzymes, apoptosis and proliferation in the toad R. arenarum. For this purpose, testicular fragments were treated during 24h with or without 2 or 20nM of E2. After treatments, the activities of cytochrome P450 17α-hydroxylase-C17-20 lyase (CypP450c17) and 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD/I) were measured by the transformation of radioactive substrates into products, and CypP450c17 expression was determined by Western blot analysis. Apoptosis in testicular sections was detected with a commercial fluorescent kit based on TUNEL method, and proliferation was evaluated by BrdU incorporation. Results indicate that E2 has no effect on CypP450c17 protein levels or enzymatic activity, while it reduces 3β-HSD/I activity during the post reproductive season. Furthermore, although E2 has no effect on apoptosis during the pre and the post reproductive seasons, it stimulates testicular apoptosis during the reproductive season, mostly in spermatocytes. Finally, E2 has no effect on testicular proliferation all year long. Taken together, these results suggest that E2 is involved in the regulation of testicular steroidogenesis and spermatogenesis. © 2015 Elsevier Inc.
format	JOUR
author	Scaia, M.F. Volonteri, M.C. Czuchlej, S.C. Ceballos, N.R.
author_facet	Scaia, M.F. Volonteri, M.C. Czuchlej, S.C. Ceballos, N.R.
author_sort	Scaia, M.F.
title	Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title_short	Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title_full	Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title_fullStr	Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title_full_unstemmed	Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)
title_sort	effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad rhinella arenarum (amphibia, anura)
url	http://hdl.handle.net/20.500.12110/paper_00166480_v221_n_p244_Scaia
work_keys_str_mv	AT scaiamf effectofestradiolonapoptosisproliferationandsteroidogenicenzymesinthetestesofthetoadrhinellaarenarumamphibiaanura AT volonterimc effectofestradiolonapoptosisproliferationandsteroidogenicenzymesinthetestesofthetoadrhinellaarenarumamphibiaanura AT czuchlejsc effectofestradiolonapoptosisproliferationandsteroidogenicenzymesinthetestesofthetoadrhinellaarenarumamphibiaanura AT ceballosnr effectofestradiolonapoptosisproliferationandsteroidogenicenzymesinthetestesofthetoadrhinellaarenarumamphibiaanura
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Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura)

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