Mechanism of hCG-induced spermiation in the toad Rhinella arenarum (Amphibia, Anura)

In Rhinella arenarum spermiation occurs as a consequence of LH/FSH increase during the amplexus or by a single dose of hCG, among other gonadotropins. The present study employs an in vitro system to study the mechanism of action of hCG in the spermiation of R. arenarum. Testicular fragments were inc...

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Autores principales:	Volonteri, M.C., Ceballos, N.R.
Formato:	JOUR
Materias:	HCG PKA Spermiation Toad adenosine triphosphatase (potassium sodium) amiloride chorionic gonadotropin cyclic AMP dependent protein kinase cyclic AMP dependent protein kinase inhibitor ouabain protein kinase C inhibitor Amphibia animal tissue article concentration response controlled study enzyme activation enzyme activity enzyme inhibition hormone action hormone release in vitro study male nonhuman priority journal Rhinella arenarum sodium transport spermatogenesis spermiation steroidogenesis
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00166480_v169_n3_p197_Volonteri
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00166480_v169_n3_p197_Volonteri
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spelling	todo:paper_00166480_v169_n3_p197_Volonteri2023-10-03T14:14:02Z Mechanism of hCG-induced spermiation in the toad Rhinella arenarum (Amphibia, Anura) Volonteri, M.C. Ceballos, N.R. HCG PKA Spermiation Toad adenosine triphosphatase (potassium sodium) amiloride chorionic gonadotropin cyclic AMP dependent protein kinase cyclic AMP dependent protein kinase inhibitor ouabain protein kinase C inhibitor Amphibia animal tissue article concentration response controlled study enzyme activation enzyme activity enzyme inhibition hormone action hormone release in vitro study male nonhuman priority journal Rhinella arenarum sodium transport spermatogenesis spermiation steroidogenesis In Rhinella arenarum spermiation occurs as a consequence of LH/FSH increase during the amplexus or by a single dose of hCG, among other gonadotropins. The present study employs an in vitro system to study the mechanism of action of hCG in the spermiation of R. arenarum. Testicular fragments were incubated for 2h at 28°C in the presence or absence of 20IU hCG with or without different PKA/PKC inhibitors and activators as well as ouabain and amiloride as Na+/K+ ATPase and transcellular Na+ transport inhibitors, respectively. Ouabain did not induce spermiation in absence of hCG and inhibited hCG-induced spermiation in a dose-dependent manner, reaching 90% inhibition with the higher concentration. In contrast, amiloride neither affected spermiation nor steroidogenesis. Activation of PKA with 8Br-cAMP induced spermiation in the absence of hCG while its inhibition with H89 blocked hCG action. On the other hand, PKC inhibition with Bi or STP did not affect hCG-induced spermiation although PKC activation significantly decreased hCG-dependent sperm release. These results suggest that PKC inhibits spermiation but also that the inhibition exerted by the kinase could be blocked by hCG. Taken together, these observations could indicate that PKA is involved in the mechanism of the gonadotropin action, mechanism also requiring the activation of a non-pumping Na+/K+ ATPase pathway. © 2010 Elsevier Inc. Fil:Volonteri, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00166480_v169_n3_p197_Volonteri
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	HCG PKA Spermiation Toad adenosine triphosphatase (potassium sodium) amiloride chorionic gonadotropin cyclic AMP dependent protein kinase cyclic AMP dependent protein kinase inhibitor ouabain protein kinase C inhibitor Amphibia animal tissue article concentration response controlled study enzyme activation enzyme activity enzyme inhibition hormone action hormone release in vitro study male nonhuman priority journal Rhinella arenarum sodium transport spermatogenesis spermiation steroidogenesis
spellingShingle	HCG PKA Spermiation Toad adenosine triphosphatase (potassium sodium) amiloride chorionic gonadotropin cyclic AMP dependent protein kinase cyclic AMP dependent protein kinase inhibitor ouabain protein kinase C inhibitor Amphibia animal tissue article concentration response controlled study enzyme activation enzyme activity enzyme inhibition hormone action hormone release in vitro study male nonhuman priority journal Rhinella arenarum sodium transport spermatogenesis spermiation steroidogenesis Volonteri, M.C. Ceballos, N.R. Mechanism of hCG-induced spermiation in the toad Rhinella arenarum (Amphibia, Anura)
topic_facet	HCG PKA Spermiation Toad adenosine triphosphatase (potassium sodium) amiloride chorionic gonadotropin cyclic AMP dependent protein kinase cyclic AMP dependent protein kinase inhibitor ouabain protein kinase C inhibitor Amphibia animal tissue article concentration response controlled study enzyme activation enzyme activity enzyme inhibition hormone action hormone release in vitro study male nonhuman priority journal Rhinella arenarum sodium transport spermatogenesis spermiation steroidogenesis
description	In Rhinella arenarum spermiation occurs as a consequence of LH/FSH increase during the amplexus or by a single dose of hCG, among other gonadotropins. The present study employs an in vitro system to study the mechanism of action of hCG in the spermiation of R. arenarum. Testicular fragments were incubated for 2h at 28°C in the presence or absence of 20IU hCG with or without different PKA/PKC inhibitors and activators as well as ouabain and amiloride as Na+/K+ ATPase and transcellular Na+ transport inhibitors, respectively. Ouabain did not induce spermiation in absence of hCG and inhibited hCG-induced spermiation in a dose-dependent manner, reaching 90% inhibition with the higher concentration. In contrast, amiloride neither affected spermiation nor steroidogenesis. Activation of PKA with 8Br-cAMP induced spermiation in the absence of hCG while its inhibition with H89 blocked hCG action. On the other hand, PKC inhibition with Bi or STP did not affect hCG-induced spermiation although PKC activation significantly decreased hCG-dependent sperm release. These results suggest that PKC inhibits spermiation but also that the inhibition exerted by the kinase could be blocked by hCG. Taken together, these observations could indicate that PKA is involved in the mechanism of the gonadotropin action, mechanism also requiring the activation of a non-pumping Na+/K+ ATPase pathway. © 2010 Elsevier Inc.
format	JOUR
author	Volonteri, M.C. Ceballos, N.R.
author_facet	Volonteri, M.C. Ceballos, N.R.
author_sort	Volonteri, M.C.
title	Mechanism of hCG-induced spermiation in the toad Rhinella arenarum (Amphibia, Anura)
title_short	Mechanism of hCG-induced spermiation in the toad Rhinella arenarum (Amphibia, Anura)
title_full	Mechanism of hCG-induced spermiation in the toad Rhinella arenarum (Amphibia, Anura)
title_fullStr	Mechanism of hCG-induced spermiation in the toad Rhinella arenarum (Amphibia, Anura)
title_full_unstemmed	Mechanism of hCG-induced spermiation in the toad Rhinella arenarum (Amphibia, Anura)
title_sort	mechanism of hcg-induced spermiation in the toad rhinella arenarum (amphibia, anura)
url	http://hdl.handle.net/20.500.12110/paper_00166480_v169_n3_p197_Volonteri
work_keys_str_mv	AT volonterimc mechanismofhcginducedspermiationinthetoadrhinellaarenarumamphibiaanura AT ceballosnr mechanismofhcginducedspermiationinthetoadrhinellaarenarumamphibiaanura
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Mechanism of hCG-induced spermiation in the toad Rhinella arenarum (Amphibia, Anura)

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