Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis
This study employed an in vitro system to identify potential steroidal mediators of spermiation in Bufo arenarum. Testicular fragments were incubated for 2 h at 28°. Spermiation was induced by 10 IU human chorionic gonadotropin (hCG) and the effect of different inhibitors of steroid biosynthesis was...
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todo:paper_00166480_v119_n2_p164_Pozzi2023-10-03T14:14:00Z Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis Pozzi, A.G. Ceballos, N.R. HCG Spermiation Steroids Toad 17 hydroxypregnenolone 17,20beta dihydroxypregn 4 en 3 one aminoglutethimide chorionic gonadotropin cyanoketone estradiol progesterone spironolactone steroid steroid 17,20 lyase steroid 17alpha monooxygenase steroid hormone receptor blocking agent testosterone animal experiment article enzyme activity enzyme inhibition inhibition kinetics nonhuman priority journal spermatogenesis steroidogenesis testis toad This study employed an in vitro system to identify potential steroidal mediators of spermiation in Bufo arenarum. Testicular fragments were incubated for 2 h at 28°. Spermiation was induced by 10 IU human chorionic gonadotropin (hCG) and the effect of different inhibitors of steroid biosynthesis was analyzed. Cyanoketone (10-5-10-6 M), an inhibitor of 3-oxo-4-ene steroid biosynthesis, did not block hCG-inducing activity even when biosynthesis of 3-oxo-4-ene steroids and its reduced metabolites was inhibited by 95%. Aminogluthetimide at a concentration that inhibited testosterone biosynthesis (10-4 and 10-5 M) did not alter hCG actions. Similar results were obtained with spironolactone, an inhibitor of 17α-hydroxylase/17-20 lyase activity. No spermiation-inducing activity was found with different steroids (progesterone, 17-hydroxypregnenolone, 17,20α/β-dihydroxy-4-pregnene-3-one, estradiol, testosterone, etc.). It is concluded that spermiation induced by hCG is not steroid mediated in B. arenarum. (C) 2000 Academic Press. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00166480_v119_n2_p164_Pozzi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
HCG Spermiation Steroids Toad 17 hydroxypregnenolone 17,20beta dihydroxypregn 4 en 3 one aminoglutethimide chorionic gonadotropin cyanoketone estradiol progesterone spironolactone steroid steroid 17,20 lyase steroid 17alpha monooxygenase steroid hormone receptor blocking agent testosterone animal experiment article enzyme activity enzyme inhibition inhibition kinetics nonhuman priority journal spermatogenesis steroidogenesis testis toad |
| spellingShingle |
HCG Spermiation Steroids Toad 17 hydroxypregnenolone 17,20beta dihydroxypregn 4 en 3 one aminoglutethimide chorionic gonadotropin cyanoketone estradiol progesterone spironolactone steroid steroid 17,20 lyase steroid 17alpha monooxygenase steroid hormone receptor blocking agent testosterone animal experiment article enzyme activity enzyme inhibition inhibition kinetics nonhuman priority journal spermatogenesis steroidogenesis testis toad Pozzi, A.G. Ceballos, N.R. Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis |
| topic_facet |
HCG Spermiation Steroids Toad 17 hydroxypregnenolone 17,20beta dihydroxypregn 4 en 3 one aminoglutethimide chorionic gonadotropin cyanoketone estradiol progesterone spironolactone steroid steroid 17,20 lyase steroid 17alpha monooxygenase steroid hormone receptor blocking agent testosterone animal experiment article enzyme activity enzyme inhibition inhibition kinetics nonhuman priority journal spermatogenesis steroidogenesis testis toad |
| description |
This study employed an in vitro system to identify potential steroidal mediators of spermiation in Bufo arenarum. Testicular fragments were incubated for 2 h at 28°. Spermiation was induced by 10 IU human chorionic gonadotropin (hCG) and the effect of different inhibitors of steroid biosynthesis was analyzed. Cyanoketone (10-5-10-6 M), an inhibitor of 3-oxo-4-ene steroid biosynthesis, did not block hCG-inducing activity even when biosynthesis of 3-oxo-4-ene steroids and its reduced metabolites was inhibited by 95%. Aminogluthetimide at a concentration that inhibited testosterone biosynthesis (10-4 and 10-5 M) did not alter hCG actions. Similar results were obtained with spironolactone, an inhibitor of 17α-hydroxylase/17-20 lyase activity. No spermiation-inducing activity was found with different steroids (progesterone, 17-hydroxypregnenolone, 17,20α/β-dihydroxy-4-pregnene-3-one, estradiol, testosterone, etc.). It is concluded that spermiation induced by hCG is not steroid mediated in B. arenarum. (C) 2000 Academic Press. |
| format |
JOUR |
| author |
Pozzi, A.G. Ceballos, N.R. |
| author_facet |
Pozzi, A.G. Ceballos, N.R. |
| author_sort |
Pozzi, A.G. |
| title |
Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis |
| title_short |
Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis |
| title_full |
Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis |
| title_fullStr |
Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis |
| title_full_unstemmed |
Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis |
| title_sort |
human chorionic gonadotropin-induced spermiation in bufo arenarum is not mediated by steroid biosynthesis |
| url |
http://hdl.handle.net/20.500.12110/paper_00166480_v119_n2_p164_Pozzi |
| work_keys_str_mv |
AT pozziag humanchorionicgonadotropininducedspermiationinbufoarenarumisnotmediatedbysteroidbiosynthesis AT ceballosnr humanchorionicgonadotropininducedspermiationinbufoarenarumisnotmediatedbysteroidbiosynthesis |
| _version_ |
1782029058960261120 |