Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis
The influence of different cultivation conditions on β-glucosidase production and of some parameters on the activity and stability of this enzyme were studied in Nectria catalinensis. Maximal β-glucosidase production was achieved with ammonium nitrate (0.5 g N/L) as nitrogen source. Tween 80, Tween...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00155632_v44_n1_p71_Pardo |
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todo:paper_00155632_v44_n1_p71_Pardo2023-10-03T14:13:36Z Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis Pardo, A.G. Forchiassin, F. Arrhenius The influence of different cultivation conditions on β-glucosidase production and of some parameters on the activity and stability of this enzyme were studied in Nectria catalinensis. Maximal β-glucosidase production was achieved with ammonium nitrate (0.5 g N/L) as nitrogen source. Tween 80, Tween 20 and Triton X-100 increased β-glucosidase yields, Tween 80 was the most effective for enzyme release and growth at a concentration of 3.4 mmol/L. On the other hand, Tween 20 and Triton X-100 had an inhibitory effect on N. catalinensis growth. A temperature of 23 °C and an initial pH of cultures of 6.5 were optimal for biomass and β-glucosidase production. Under optimal cultural conditions (ammonium nitrate, 0.5 g N/L; Tween 80, 3.4 mmol/L; 23 °C; initial pH 6.5) the β-glucosidase yield was increased more than five fold respect to the initial state. Optimal temperature for β-glucosidase activity was 45 °C, the initial activity dropped 60 % after 6 h of incubation at this temperature. Optimal PH for enzyme activity was 5.3. At this pH the β-glucosidase was completely stable after 3 d of incubation The V and Km values calculated from Lineweaver-Burk and Eadie-Hofstee plots were 0.23 μmol 4-nitrophenol per min per mg of protein and 0.25 mmol 4-nitrophenol β-D-glucopyranoside per L, respectively. The activation energy according to Arrhenius plot was 49.6 KJ/mol. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00155632_v44_n1_p71_Pardo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arrhenius |
| spellingShingle |
Arrhenius Pardo, A.G. Forchiassin, F. Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis |
| topic_facet |
Arrhenius |
| description |
The influence of different cultivation conditions on β-glucosidase production and of some parameters on the activity and stability of this enzyme were studied in Nectria catalinensis. Maximal β-glucosidase production was achieved with ammonium nitrate (0.5 g N/L) as nitrogen source. Tween 80, Tween 20 and Triton X-100 increased β-glucosidase yields, Tween 80 was the most effective for enzyme release and growth at a concentration of 3.4 mmol/L. On the other hand, Tween 20 and Triton X-100 had an inhibitory effect on N. catalinensis growth. A temperature of 23 °C and an initial pH of cultures of 6.5 were optimal for biomass and β-glucosidase production. Under optimal cultural conditions (ammonium nitrate, 0.5 g N/L; Tween 80, 3.4 mmol/L; 23 °C; initial pH 6.5) the β-glucosidase yield was increased more than five fold respect to the initial state. Optimal temperature for β-glucosidase activity was 45 °C, the initial activity dropped 60 % after 6 h of incubation at this temperature. Optimal PH for enzyme activity was 5.3. At this pH the β-glucosidase was completely stable after 3 d of incubation The V and Km values calculated from Lineweaver-Burk and Eadie-Hofstee plots were 0.23 μmol 4-nitrophenol per min per mg of protein and 0.25 mmol 4-nitrophenol β-D-glucopyranoside per L, respectively. The activation energy according to Arrhenius plot was 49.6 KJ/mol. |
| format |
JOUR |
| author |
Pardo, A.G. Forchiassin, F. |
| author_facet |
Pardo, A.G. Forchiassin, F. |
| author_sort |
Pardo, A.G. |
| title |
Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis |
| title_short |
Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis |
| title_full |
Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis |
| title_fullStr |
Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis |
| title_full_unstemmed |
Factors influencing β-glucosidase production, activity and stability in Nectria catalinensis |
| title_sort |
factors influencing β-glucosidase production, activity and stability in nectria catalinensis |
| url |
http://hdl.handle.net/20.500.12110/paper_00155632_v44_n1_p71_Pardo |
| work_keys_str_mv |
AT pardoag factorsinfluencingbglucosidaseproductionactivityandstabilityinnectriacatalinensis AT forchiassinf factorsinfluencingbglucosidaseproductionactivityandstabilityinnectriacatalinensis |
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1807321462742188032 |