Comparison of mating performance of medfly (Diptera: Tephritidae) genetic sexing and wild type strains: Field cage and video recording experiments

To improve the efficiency of the sterile insect technique (SIT) efforts are being devoted to obtain genetic sexing strains (GSS). The present work was carried out in order to compare the mating efficiency of flies from the GSS [(Ty34228 y+/X)swx] and from a wild type strain (Mendoza). Females of the...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Calcagno, G.E., Manso, F., Vilardi, J.C.
Formato: JOUR
Materias:
Copulatory success
Mating behavior
Sexual selection
Sperm transfer
biological control
copulation
mating behavior
rearing
sterile release method
videography
Capitata
Ceratitis
Ceratitis capitata
Diptera
Drosophila melanogaster
Insecta
Tephritidae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00154040_v85_n1_p41_Calcagno
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00154040_v85_n1_p41_Calcagno
record_format dspace
spelling todo:paper_00154040_v85_n1_p41_Calcagno2023-10-03T14:13:32Z Comparison of mating performance of medfly (Diptera: Tephritidae) genetic sexing and wild type strains: Field cage and video recording experiments Calcagno, G.E. Manso, F. Vilardi, J.C. Copulatory success Mating behavior Sexual selection Sperm transfer biological control copulation mating behavior rearing sterile release method videography Capitata Ceratitis Ceratitis capitata Diptera Drosophila melanogaster Insecta Tephritidae To improve the efficiency of the sterile insect technique (SIT) efforts are being devoted to obtain genetic sexing strains (GSS). The present work was carried out in order to compare the mating efficiency of flies from the GSS [(Ty34228 y+/X)swx] and from a wild type strain (Mendoza). Females of the GSS (T228) exhibit longer embryonic development, while males develop in a normal time period. In a field-cage experiment, mating competitiveness was compared between the T228 and the Mendoza, Argentina mass reared strain. The number and duration of matings and the location of copula in the tree were recorded. The analysis was repeated using irradiated males of T228. The results showed that mating efficiency of the GSS is good in comparison with that of the Mendoza strain. Although copulatory success in T228 is reduced by the radiation treatment, the high numbers of sterilized males released would compensate this effect in the control programs. In a second experiment, under laboratory conditions, videorecording techniques were applied. In this case two virgin males, one of the GSS and one emerged from wild collected fruits, competed during 30 min for a virgin wild female. The proportion of successful males did not differ between strains, but some differences were observed between strains in the time spent in different stages of the courtship. Males of the T228 were more aggressive, and they attempted to copulate with the other male more frequently than did wild males. These differences may be due to selection for more aggressive individuals under the overcrowded laboratory breeding conditions for this strain. Fil:Calcagno, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vilardi, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00154040_v85_n1_p41_Calcagno
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Copulatory success
Mating behavior
Sexual selection
Sperm transfer
biological control
copulation
mating behavior
rearing
sterile release method
videography
Capitata
Ceratitis
Ceratitis capitata
Diptera
Drosophila melanogaster
Insecta
Tephritidae
spellingShingle Copulatory success
Mating behavior
Sexual selection
Sperm transfer
biological control
copulation
mating behavior
rearing
sterile release method
videography
Capitata
Ceratitis
Ceratitis capitata
Diptera
Drosophila melanogaster
Insecta
Tephritidae
Calcagno, G.E.
Manso, F.
Vilardi, J.C.
Comparison of mating performance of medfly (Diptera: Tephritidae) genetic sexing and wild type strains: Field cage and video recording experiments
topic_facet Copulatory success
Mating behavior
Sexual selection
Sperm transfer
biological control
copulation
mating behavior
rearing
sterile release method
videography
Capitata
Ceratitis
Ceratitis capitata
Diptera
Drosophila melanogaster
Insecta
Tephritidae
description To improve the efficiency of the sterile insect technique (SIT) efforts are being devoted to obtain genetic sexing strains (GSS). The present work was carried out in order to compare the mating efficiency of flies from the GSS [(Ty34228 y+/X)swx] and from a wild type strain (Mendoza). Females of the GSS (T228) exhibit longer embryonic development, while males develop in a normal time period. In a field-cage experiment, mating competitiveness was compared between the T228 and the Mendoza, Argentina mass reared strain. The number and duration of matings and the location of copula in the tree were recorded. The analysis was repeated using irradiated males of T228. The results showed that mating efficiency of the GSS is good in comparison with that of the Mendoza strain. Although copulatory success in T228 is reduced by the radiation treatment, the high numbers of sterilized males released would compensate this effect in the control programs. In a second experiment, under laboratory conditions, videorecording techniques were applied. In this case two virgin males, one of the GSS and one emerged from wild collected fruits, competed during 30 min for a virgin wild female. The proportion of successful males did not differ between strains, but some differences were observed between strains in the time spent in different stages of the courtship. Males of the T228 were more aggressive, and they attempted to copulate with the other male more frequently than did wild males. These differences may be due to selection for more aggressive individuals under the overcrowded laboratory breeding conditions for this strain.
format JOUR
author Calcagno, G.E.
Manso, F.
Vilardi, J.C.
author_facet Calcagno, G.E.
Manso, F.
Vilardi, J.C.
author_sort Calcagno, G.E.
title Comparison of mating performance of medfly (Diptera: Tephritidae) genetic sexing and wild type strains: Field cage and video recording experiments
title_short Comparison of mating performance of medfly (Diptera: Tephritidae) genetic sexing and wild type strains: Field cage and video recording experiments
title_full Comparison of mating performance of medfly (Diptera: Tephritidae) genetic sexing and wild type strains: Field cage and video recording experiments
title_fullStr Comparison of mating performance of medfly (Diptera: Tephritidae) genetic sexing and wild type strains: Field cage and video recording experiments
title_full_unstemmed Comparison of mating performance of medfly (Diptera: Tephritidae) genetic sexing and wild type strains: Field cage and video recording experiments
title_sort comparison of mating performance of medfly (diptera: tephritidae) genetic sexing and wild type strains: field cage and video recording experiments
url http://hdl.handle.net/20.500.12110/paper_00154040_v85_n1_p41_Calcagno
work_keys_str_mv AT calcagnoge comparisonofmatingperformanceofmedflydipteratephritidaegeneticsexingandwildtypestrainsfieldcageandvideorecordingexperiments
AT mansof comparisonofmatingperformanceofmedflydipteratephritidaegeneticsexingandwildtypestrainsfieldcageandvideorecordingexperiments
AT vilardijc comparisonofmatingperformanceofmedflydipteratephritidaegeneticsexingandwildtypestrainsfieldcageandvideorecordingexperiments
_version_ 1782028038361317376

Ejemplares similares