Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the PI3K/AKT signaling pathway
Objective: To investigate the effects of local inhibition of vascular endothelial growth factor A (VEGFA) on proliferation and apoptosis of follicular cells in rat ovaries. To analyze the role of the PI3K/AKT signaling pathway on VEGFA effects. Design: Experimental study. Setting: Research laborator...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00150282_v93_n5_p1369_Abramovich |
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todo:paper_00150282_v93_n5_p1369_Abramovich2023-10-03T14:13:30Z Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the PI3K/AKT signaling pathway Abramovich, D. Irusta, G. Parborell, F. Tesone, M. angiogenesis apoptosis Ovary PI3K/AKT proliferation VEGFA caspase 3 chorionic gonadotropin cycline phosphatidylinositol 3 kinase protein bcl 2 protein kinase B Rac protein serine threonine vasculotropin vasculotropin receptor 2 animal experiment animal tissue apoptosis article cell death cell density cell growth cell maturation cell proliferation controlled study endothelium cell female gene expression regulation granulosa cell inhibition kinetics nonhuman ovary follicle cell prepuberty priority journal protein phosphorylation rat signal transduction theca cell 1-Phosphatidylinositol 3-Kinase Animals Apoptosis bcl-Associated Death Protein Caspase 3 Cell Proliferation Endothelial Cells Enzyme Activation Female Gonadotropins, Equine Injections Ovarian Follicle Ovary Phosphorylation Proto-Oncogene Proteins c-akt Rats Rats, Sprague-Dawley Receptors, Vascular Endothelial Growth Factor Recombinant Fusion Proteins Sexual Maturation Signal Transduction Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-2 Objective: To investigate the effects of local inhibition of vascular endothelial growth factor A (VEGFA) on proliferation and apoptosis of follicular cells in rat ovaries. To analyze the role of the PI3K/AKT signaling pathway on VEGFA effects. Design: Experimental study. Setting: Research laboratory. Animal(s): Female Sprague Dawley rats, 21 days old, treated with equine chorionic gonadotropin (eCG). Main Outcome Measure(s): Follicular cell proliferation, apoptosis, and activation of the PI3K/AKT signaling pathway after intrabursal injection of a VEGFA inhibitor. Result(s): Inhibition of VEGFA leads to a decrease in the expression of the proliferation marker proliferating cell nuclear antigen (PCNA) in theca and granulosa cells (GC) and an increase in the activation of caspase 3 in antral follicles. Furthermore, we observed a decrease in the phosphorylation of RAC-alpha serine/threonine-protein kinase (AKT) and its target Bcl2 antagonist of cell death (BAD). No differences were found in the levels of kinase insert domain receptor (KDR) protein or in endothelial cell density. Conclusion(s): The VEGFA prevents apoptosis and stimulates proliferation of follicular cells, regulating follicular growth and development. The PI3K/AKT signaling pathway is one of the pathways involved in this mechanism. Therefore, VEGFA has a role as an antiapoptotic and proliferative factor in follicular cells from the rat ovary. © 2010 American Society for Reproductive Medicine. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00150282_v93_n5_p1369_Abramovich |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
angiogenesis apoptosis Ovary PI3K/AKT proliferation VEGFA caspase 3 chorionic gonadotropin cycline phosphatidylinositol 3 kinase protein bcl 2 protein kinase B Rac protein serine threonine vasculotropin vasculotropin receptor 2 animal experiment animal tissue apoptosis article cell death cell density cell growth cell maturation cell proliferation controlled study endothelium cell female gene expression regulation granulosa cell inhibition kinetics nonhuman ovary follicle cell prepuberty priority journal protein phosphorylation rat signal transduction theca cell 1-Phosphatidylinositol 3-Kinase Animals Apoptosis bcl-Associated Death Protein Caspase 3 Cell Proliferation Endothelial Cells Enzyme Activation Female Gonadotropins, Equine Injections Ovarian Follicle Ovary Phosphorylation Proto-Oncogene Proteins c-akt Rats Rats, Sprague-Dawley Receptors, Vascular Endothelial Growth Factor Recombinant Fusion Proteins Sexual Maturation Signal Transduction Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-2 |
| spellingShingle |
angiogenesis apoptosis Ovary PI3K/AKT proliferation VEGFA caspase 3 chorionic gonadotropin cycline phosphatidylinositol 3 kinase protein bcl 2 protein kinase B Rac protein serine threonine vasculotropin vasculotropin receptor 2 animal experiment animal tissue apoptosis article cell death cell density cell growth cell maturation cell proliferation controlled study endothelium cell female gene expression regulation granulosa cell inhibition kinetics nonhuman ovary follicle cell prepuberty priority journal protein phosphorylation rat signal transduction theca cell 1-Phosphatidylinositol 3-Kinase Animals Apoptosis bcl-Associated Death Protein Caspase 3 Cell Proliferation Endothelial Cells Enzyme Activation Female Gonadotropins, Equine Injections Ovarian Follicle Ovary Phosphorylation Proto-Oncogene Proteins c-akt Rats Rats, Sprague-Dawley Receptors, Vascular Endothelial Growth Factor Recombinant Fusion Proteins Sexual Maturation Signal Transduction Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-2 Abramovich, D. Irusta, G. Parborell, F. Tesone, M. Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the PI3K/AKT signaling pathway |
| topic_facet |
angiogenesis apoptosis Ovary PI3K/AKT proliferation VEGFA caspase 3 chorionic gonadotropin cycline phosphatidylinositol 3 kinase protein bcl 2 protein kinase B Rac protein serine threonine vasculotropin vasculotropin receptor 2 animal experiment animal tissue apoptosis article cell death cell density cell growth cell maturation cell proliferation controlled study endothelium cell female gene expression regulation granulosa cell inhibition kinetics nonhuman ovary follicle cell prepuberty priority journal protein phosphorylation rat signal transduction theca cell 1-Phosphatidylinositol 3-Kinase Animals Apoptosis bcl-Associated Death Protein Caspase 3 Cell Proliferation Endothelial Cells Enzyme Activation Female Gonadotropins, Equine Injections Ovarian Follicle Ovary Phosphorylation Proto-Oncogene Proteins c-akt Rats Rats, Sprague-Dawley Receptors, Vascular Endothelial Growth Factor Recombinant Fusion Proteins Sexual Maturation Signal Transduction Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-2 |
| description |
Objective: To investigate the effects of local inhibition of vascular endothelial growth factor A (VEGFA) on proliferation and apoptosis of follicular cells in rat ovaries. To analyze the role of the PI3K/AKT signaling pathway on VEGFA effects. Design: Experimental study. Setting: Research laboratory. Animal(s): Female Sprague Dawley rats, 21 days old, treated with equine chorionic gonadotropin (eCG). Main Outcome Measure(s): Follicular cell proliferation, apoptosis, and activation of the PI3K/AKT signaling pathway after intrabursal injection of a VEGFA inhibitor. Result(s): Inhibition of VEGFA leads to a decrease in the expression of the proliferation marker proliferating cell nuclear antigen (PCNA) in theca and granulosa cells (GC) and an increase in the activation of caspase 3 in antral follicles. Furthermore, we observed a decrease in the phosphorylation of RAC-alpha serine/threonine-protein kinase (AKT) and its target Bcl2 antagonist of cell death (BAD). No differences were found in the levels of kinase insert domain receptor (KDR) protein or in endothelial cell density. Conclusion(s): The VEGFA prevents apoptosis and stimulates proliferation of follicular cells, regulating follicular growth and development. The PI3K/AKT signaling pathway is one of the pathways involved in this mechanism. Therefore, VEGFA has a role as an antiapoptotic and proliferative factor in follicular cells from the rat ovary. © 2010 American Society for Reproductive Medicine. |
| format |
JOUR |
| author |
Abramovich, D. Irusta, G. Parborell, F. Tesone, M. |
| author_facet |
Abramovich, D. Irusta, G. Parborell, F. Tesone, M. |
| author_sort |
Abramovich, D. |
| title |
Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the PI3K/AKT signaling pathway |
| title_short |
Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the PI3K/AKT signaling pathway |
| title_full |
Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the PI3K/AKT signaling pathway |
| title_fullStr |
Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the PI3K/AKT signaling pathway |
| title_full_unstemmed |
Intrabursal injection of vascular endothelial growth factor trap in eCG-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the PI3K/AKT signaling pathway |
| title_sort |
intrabursal injection of vascular endothelial growth factor trap in ecg-treated prepubertal rats inhibits proliferation and increases apoptosis of follicular cells involving the pi3k/akt signaling pathway |
| url |
http://hdl.handle.net/20.500.12110/paper_00150282_v93_n5_p1369_Abramovich |
| work_keys_str_mv |
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