Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis
Objective: To evaluate and compare spontaneous apoptosis and Bcl-2 and Bax expression in eutopic endometrium from women with and without endometriosis. Design: Apoptosis and Bcl-2 and Bax expression were examined in eutopic endometrium from women with and without endometriosis. Setting: Instituto de...
Guardado en:
Autores principales: | , , , , , |
---|---|
Formato: | JOUR |
Materias: | |
Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00150282_v74_n4_p760_Meresman |
Aporte de: |
id |
todo:paper_00150282_v74_n4_p760_Meresman |
---|---|
record_format |
dspace |
spelling |
todo:paper_00150282_v74_n4_p760_Meresman2023-10-03T14:13:23Z Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis Meresman, G.F. Vighi, S. Buquet, R.A. Contreras-Ortiz, O. Tesone, M. Rumi, L.S. Apoptosis Bax Bcl-2 Endometriosis Eutopic endometrium protein Bax protein bcl 2 apoptosis article clinical article controlled study endometriosis female human human tissue immunohistochemistry laparoscopy priority journal protein expression Apoptosis Endometriosis Endometrium Female Human In Situ Hybridization Menstrual Cycle Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Support, Non-U.S. Gov't Objective: To evaluate and compare spontaneous apoptosis and Bcl-2 and Bax expression in eutopic endometrium from women with and without endometriosis. Design: Apoptosis and Bcl-2 and Bax expression were examined in eutopic endometrium from women with and without endometriosis. Setting: Instituto de Biologia y Medicina Experimental-CONICET, Department of Gynecology and Department of Gynecological Pathology, Clinicas University Hospital, Buenos Aires, Argentina. Patient(s): Women with untreated endometriosis (n = 14) and controls (n = 16). Intervention(s): Collection of endometrial samples during diagnostic or therapeutic laparoscopy. Main Outcome Measure(s): Apoptotic cells were detected with use of the dUTP nick- end labeling (TUNEL) assay; Bcl-2 and Bax expressions were assessed with use of immunohistochemical techniques. Result(s): Spontaneous apoptosis was significantly lower in eutopic endometrium from patients with endometriosis, compared with healthy controls (2.26 ± 0.53 and 9.37 ± 1.69 apoptotic cells/field, respectively) and was independent of cycle phase. An increased expression of Bcl-2 protein was found in proliferative eutopic endometrium from patients with endometriosis. Bax expression was absent in proliferative endometrium, whereas there was an increase in its expression in secretory endometrium from both patients and controls. Conclusion(s): Women with endometriosis show decreased number of apoptotic cells in eutopic endometrium. The abnormal survival of endometrial cells may result in their continuing growth into ectopic locations. (C) 2000 by American Society for Reproductive Medicine. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00150282_v74_n4_p760_Meresman |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Apoptosis Bax Bcl-2 Endometriosis Eutopic endometrium protein Bax protein bcl 2 apoptosis article clinical article controlled study endometriosis female human human tissue immunohistochemistry laparoscopy priority journal protein expression Apoptosis Endometriosis Endometrium Female Human In Situ Hybridization Menstrual Cycle Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Support, Non-U.S. Gov't |
spellingShingle |
Apoptosis Bax Bcl-2 Endometriosis Eutopic endometrium protein Bax protein bcl 2 apoptosis article clinical article controlled study endometriosis female human human tissue immunohistochemistry laparoscopy priority journal protein expression Apoptosis Endometriosis Endometrium Female Human In Situ Hybridization Menstrual Cycle Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Support, Non-U.S. Gov't Meresman, G.F. Vighi, S. Buquet, R.A. Contreras-Ortiz, O. Tesone, M. Rumi, L.S. Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis |
topic_facet |
Apoptosis Bax Bcl-2 Endometriosis Eutopic endometrium protein Bax protein bcl 2 apoptosis article clinical article controlled study endometriosis female human human tissue immunohistochemistry laparoscopy priority journal protein expression Apoptosis Endometriosis Endometrium Female Human In Situ Hybridization Menstrual Cycle Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Support, Non-U.S. Gov't |
description |
Objective: To evaluate and compare spontaneous apoptosis and Bcl-2 and Bax expression in eutopic endometrium from women with and without endometriosis. Design: Apoptosis and Bcl-2 and Bax expression were examined in eutopic endometrium from women with and without endometriosis. Setting: Instituto de Biologia y Medicina Experimental-CONICET, Department of Gynecology and Department of Gynecological Pathology, Clinicas University Hospital, Buenos Aires, Argentina. Patient(s): Women with untreated endometriosis (n = 14) and controls (n = 16). Intervention(s): Collection of endometrial samples during diagnostic or therapeutic laparoscopy. Main Outcome Measure(s): Apoptotic cells were detected with use of the dUTP nick- end labeling (TUNEL) assay; Bcl-2 and Bax expressions were assessed with use of immunohistochemical techniques. Result(s): Spontaneous apoptosis was significantly lower in eutopic endometrium from patients with endometriosis, compared with healthy controls (2.26 ± 0.53 and 9.37 ± 1.69 apoptotic cells/field, respectively) and was independent of cycle phase. An increased expression of Bcl-2 protein was found in proliferative eutopic endometrium from patients with endometriosis. Bax expression was absent in proliferative endometrium, whereas there was an increase in its expression in secretory endometrium from both patients and controls. Conclusion(s): Women with endometriosis show decreased number of apoptotic cells in eutopic endometrium. The abnormal survival of endometrial cells may result in their continuing growth into ectopic locations. (C) 2000 by American Society for Reproductive Medicine. |
format |
JOUR |
author |
Meresman, G.F. Vighi, S. Buquet, R.A. Contreras-Ortiz, O. Tesone, M. Rumi, L.S. |
author_facet |
Meresman, G.F. Vighi, S. Buquet, R.A. Contreras-Ortiz, O. Tesone, M. Rumi, L.S. |
author_sort |
Meresman, G.F. |
title |
Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis |
title_short |
Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis |
title_full |
Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis |
title_fullStr |
Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis |
title_full_unstemmed |
Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis |
title_sort |
apoptosis and expression of bcl-2 and bax in eutopic endometrium from women with endometriosis |
url |
http://hdl.handle.net/20.500.12110/paper_00150282_v74_n4_p760_Meresman |
work_keys_str_mv |
AT meresmangf apoptosisandexpressionofbcl2andbaxineutopicendometriumfromwomenwithendometriosis AT vighis apoptosisandexpressionofbcl2andbaxineutopicendometriumfromwomenwithendometriosis AT buquetra apoptosisandexpressionofbcl2andbaxineutopicendometriumfromwomenwithendometriosis AT contrerasortizo apoptosisandexpressionofbcl2andbaxineutopicendometriumfromwomenwithendometriosis AT tesonem apoptosisandexpressionofbcl2andbaxineutopicendometriumfromwomenwithendometriosis AT rumils apoptosisandexpressionofbcl2andbaxineutopicendometriumfromwomenwithendometriosis |
_version_ |
1782026977746616320 |