Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth
Proper cell wall assembly is crucial during pollen tube growth. Leucine-rich repeat extensins (LRXs) are extracellular glycoproteins which belong to the hydroxyproline-rich glycoprotein (HRGP) family. They contain a conserved N-terminal leucine-rich repeat (LRR) domain and a highly variable C-termin...
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todo:paper_00145793_v592_n2_p233_Sede2023-10-03T14:13:14Z Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth Sede, A.R. Borassi, C. Wengier, D.L. Mecchia, M.A. Estevez, J.M. Muschietti, J.P. callose cell wall extensin LRX pectin polarized growth pollen tube beta 1,3 glucan callose extensin leucine rich repeat extensin LRX10 protein LRX11 protein LRX8 protein LRX9 protein pectin unclassified drug callose extensin protein, plant glucan glycoprotein pectin plant protein Arabidopsis thaliana cell wall controlled study Letter nonhuman pollen germination pollen tube growth pollination priority journal seed development Arabidopsis cell wall gene expression regulation genetics germination growth, development and aging metabolism molecular cloning physiology pollen tube Arabidopsis Cell Wall Cloning, Molecular Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Germination Glucans Glycoproteins Mutagenesis, Insertional Pectins Plant Proteins Pollen Tube Proper cell wall assembly is crucial during pollen tube growth. Leucine-rich repeat extensins (LRXs) are extracellular glycoproteins which belong to the hydroxyproline-rich glycoprotein (HRGP) family. They contain a conserved N-terminal leucine-rich repeat (LRR) domain and a highly variable C-terminal extensin domain. Here, we characterized four LRX proteins (LRX8 through LRX11) from pollen of Arabidopsis thaliana. To investigate the role of LRX8-LRX11 in pollen germination and pollen tube growth, multiple T-DNA lrx mutants were obtained. The lrx mutants display abnormal pollen tubes with an irregular deposition of callose and pectin. They also show serious alterations in pollen germination and segregation ratio. Our results suggest that LRXs are involved in ensuring proper cell wall assembly during pollen tube growth. © 2017 Federation of European Biochemical Societies JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v592_n2_p233_Sede |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
callose cell wall extensin LRX pectin polarized growth pollen tube beta 1,3 glucan callose extensin leucine rich repeat extensin LRX10 protein LRX11 protein LRX8 protein LRX9 protein pectin unclassified drug callose extensin protein, plant glucan glycoprotein pectin plant protein Arabidopsis thaliana cell wall controlled study Letter nonhuman pollen germination pollen tube growth pollination priority journal seed development Arabidopsis cell wall gene expression regulation genetics germination growth, development and aging metabolism molecular cloning physiology pollen tube Arabidopsis Cell Wall Cloning, Molecular Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Germination Glucans Glycoproteins Mutagenesis, Insertional Pectins Plant Proteins Pollen Tube |
| spellingShingle |
callose cell wall extensin LRX pectin polarized growth pollen tube beta 1,3 glucan callose extensin leucine rich repeat extensin LRX10 protein LRX11 protein LRX8 protein LRX9 protein pectin unclassified drug callose extensin protein, plant glucan glycoprotein pectin plant protein Arabidopsis thaliana cell wall controlled study Letter nonhuman pollen germination pollen tube growth pollination priority journal seed development Arabidopsis cell wall gene expression regulation genetics germination growth, development and aging metabolism molecular cloning physiology pollen tube Arabidopsis Cell Wall Cloning, Molecular Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Germination Glucans Glycoproteins Mutagenesis, Insertional Pectins Plant Proteins Pollen Tube Sede, A.R. Borassi, C. Wengier, D.L. Mecchia, M.A. Estevez, J.M. Muschietti, J.P. Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth |
| topic_facet |
callose cell wall extensin LRX pectin polarized growth pollen tube beta 1,3 glucan callose extensin leucine rich repeat extensin LRX10 protein LRX11 protein LRX8 protein LRX9 protein pectin unclassified drug callose extensin protein, plant glucan glycoprotein pectin plant protein Arabidopsis thaliana cell wall controlled study Letter nonhuman pollen germination pollen tube growth pollination priority journal seed development Arabidopsis cell wall gene expression regulation genetics germination growth, development and aging metabolism molecular cloning physiology pollen tube Arabidopsis Cell Wall Cloning, Molecular Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Germination Glucans Glycoproteins Mutagenesis, Insertional Pectins Plant Proteins Pollen Tube |
| description |
Proper cell wall assembly is crucial during pollen tube growth. Leucine-rich repeat extensins (LRXs) are extracellular glycoproteins which belong to the hydroxyproline-rich glycoprotein (HRGP) family. They contain a conserved N-terminal leucine-rich repeat (LRR) domain and a highly variable C-terminal extensin domain. Here, we characterized four LRX proteins (LRX8 through LRX11) from pollen of Arabidopsis thaliana. To investigate the role of LRX8-LRX11 in pollen germination and pollen tube growth, multiple T-DNA lrx mutants were obtained. The lrx mutants display abnormal pollen tubes with an irregular deposition of callose and pectin. They also show serious alterations in pollen germination and segregation ratio. Our results suggest that LRXs are involved in ensuring proper cell wall assembly during pollen tube growth. © 2017 Federation of European Biochemical Societies |
| format |
JOUR |
| author |
Sede, A.R. Borassi, C. Wengier, D.L. Mecchia, M.A. Estevez, J.M. Muschietti, J.P. |
| author_facet |
Sede, A.R. Borassi, C. Wengier, D.L. Mecchia, M.A. Estevez, J.M. Muschietti, J.P. |
| author_sort |
Sede, A.R. |
| title |
Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth |
| title_short |
Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth |
| title_full |
Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth |
| title_fullStr |
Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth |
| title_full_unstemmed |
Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth |
| title_sort |
arabidopsis pollen extensins lrx are required for cell wall integrity during pollen tube growth |
| url |
http://hdl.handle.net/20.500.12110/paper_00145793_v592_n2_p233_Sede |
| work_keys_str_mv |
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1807320441420775424 |