Antibody response to a viral transcriptional regulator

The E2 transcriptional activator of the human papillomavirus regulates the expression of most viral transcripts. Its binding to specific target DNA sequences involves large conformational changes in the interacting macromolecules. The high stability of the E2:DNA complex prompted us to analyze the r...

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Autores principales:	Cerutti, M.L., Centeno, J.M., De Prat-Gay, G., Goldbaum, F.A.
Formato:	JOUR
Materias:	Adjuvant Antibody Antigen binding Conformational epitope Transcription factor adjuvant epitope monoclonal antibody transcription factor transcription factor E2 unclassified drug antibody response antibody specificity article DNA binding DNA determination DNA sequence emulsion enzyme regulation enzyme stability epitope mapping humoral immunity immunization macromolecule molecular recognition nonhuman Papilloma virus priority journal protein conformation protein interaction transcription initiation transcription regulation virus expression virus transcription Human papillomavirus Papillomavirus
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00145793_v534_n1-3_p202_Cerutti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00145793_v534_n1-3_p202_Cerutti
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spelling	todo:paper_00145793_v534_n1-3_p202_Cerutti2023-10-03T14:13:08Z Antibody response to a viral transcriptional regulator Cerutti, M.L. Centeno, J.M. De Prat-Gay, G. Goldbaum, F.A. Adjuvant Antibody Antigen binding Conformational epitope Transcription factor adjuvant epitope monoclonal antibody transcription factor transcription factor E2 unclassified drug antibody response antibody specificity article DNA binding DNA determination DNA sequence emulsion enzyme regulation enzyme stability epitope mapping humoral immunity immunization macromolecule molecular recognition nonhuman Papilloma virus priority journal protein conformation protein interaction transcription initiation transcription regulation virus expression virus transcription Human papillomavirus Papillomavirus The E2 transcriptional activator of the human papillomavirus regulates the expression of most viral transcripts. Its binding to specific target DNA sequences involves large conformational changes in the interacting macromolecules. The high stability of the E2:DNA complex prompted us to analyze the role of macromolecular interactions and adjuvant emulsions in the appearance of conformation-specific antibodies. We demonstrate that immunization with free or DNA-complexed E2 emulsified in an oil-in-water adjuvant elicits a humoral response shifted to the recognition of discontinuous epitopes. Epitope mapping and functional analysis of the generated anti-E2 mAbs reveals that two separate antibodies populations can be obtained: those able to form a stable ternary complex with protein and DNA, and those which recognize the DNA-binding surface of the transcription factor, interfering with E2 binding to DNA. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. Fil:Cerutti, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Prat-Gay, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v534_n1-3_p202_Cerutti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Adjuvant Antibody Antigen binding Conformational epitope Transcription factor adjuvant epitope monoclonal antibody transcription factor transcription factor E2 unclassified drug antibody response antibody specificity article DNA binding DNA determination DNA sequence emulsion enzyme regulation enzyme stability epitope mapping humoral immunity immunization macromolecule molecular recognition nonhuman Papilloma virus priority journal protein conformation protein interaction transcription initiation transcription regulation virus expression virus transcription Human papillomavirus Papillomavirus
spellingShingle	Adjuvant Antibody Antigen binding Conformational epitope Transcription factor adjuvant epitope monoclonal antibody transcription factor transcription factor E2 unclassified drug antibody response antibody specificity article DNA binding DNA determination DNA sequence emulsion enzyme regulation enzyme stability epitope mapping humoral immunity immunization macromolecule molecular recognition nonhuman Papilloma virus priority journal protein conformation protein interaction transcription initiation transcription regulation virus expression virus transcription Human papillomavirus Papillomavirus Cerutti, M.L. Centeno, J.M. De Prat-Gay, G. Goldbaum, F.A. Antibody response to a viral transcriptional regulator
topic_facet	Adjuvant Antibody Antigen binding Conformational epitope Transcription factor adjuvant epitope monoclonal antibody transcription factor transcription factor E2 unclassified drug antibody response antibody specificity article DNA binding DNA determination DNA sequence emulsion enzyme regulation enzyme stability epitope mapping humoral immunity immunization macromolecule molecular recognition nonhuman Papilloma virus priority journal protein conformation protein interaction transcription initiation transcription regulation virus expression virus transcription Human papillomavirus Papillomavirus
description	The E2 transcriptional activator of the human papillomavirus regulates the expression of most viral transcripts. Its binding to specific target DNA sequences involves large conformational changes in the interacting macromolecules. The high stability of the E2:DNA complex prompted us to analyze the role of macromolecular interactions and adjuvant emulsions in the appearance of conformation-specific antibodies. We demonstrate that immunization with free or DNA-complexed E2 emulsified in an oil-in-water adjuvant elicits a humoral response shifted to the recognition of discontinuous epitopes. Epitope mapping and functional analysis of the generated anti-E2 mAbs reveals that two separate antibodies populations can be obtained: those able to form a stable ternary complex with protein and DNA, and those which recognize the DNA-binding surface of the transcription factor, interfering with E2 binding to DNA. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
format	JOUR
author	Cerutti, M.L. Centeno, J.M. De Prat-Gay, G. Goldbaum, F.A.
author_facet	Cerutti, M.L. Centeno, J.M. De Prat-Gay, G. Goldbaum, F.A.
author_sort	Cerutti, M.L.
title	Antibody response to a viral transcriptional regulator
title_short	Antibody response to a viral transcriptional regulator
title_full	Antibody response to a viral transcriptional regulator
title_fullStr	Antibody response to a viral transcriptional regulator
title_full_unstemmed	Antibody response to a viral transcriptional regulator
title_sort	antibody response to a viral transcriptional regulator
url	http://hdl.handle.net/20.500.12110/paper_00145793_v534_n1-3_p202_Cerutti
work_keys_str_mv	AT ceruttiml antibodyresponsetoaviraltranscriptionalregulator AT centenojm antibodyresponsetoaviraltranscriptionalregulator AT depratgayg antibodyresponsetoaviraltranscriptionalregulator AT goldbaumfa antibodyresponsetoaviraltranscriptionalregulator
_version_	1782029271108157440

Antibody response to a viral transcriptional regulator

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