Trypanosoma cruzi epimastigote forms possess a Ca2+-calmodulin dependent protein kinase
Trypanosoma cruzi epimastigote forms showed a tightly bound Ca2+-calmodulin-dependent protein kinase activity, which could be partially extracted from membranes and axonemes. The enzyme is constituted by subunits which were autophosphorylated in the absence of exogenous substrates. An antibody again...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00145793_v337_n3_p293_Ogueta |
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todo:paper_00145793_v337_n3_p293_Ogueta2023-10-03T14:13:03Z Trypanosoma cruzi epimastigote forms possess a Ca2+-calmodulin dependent protein kinase Ogueta, S.B. Solari, A. Téllez-Iñón, M.T. Ca2+-calmodulin protein kinase Cytoskeleton Flagella Trypanosoma cruzi Trypanosomatid protein kinase (calcium,calmodulin) ii article cytoskeleton epimastigote flagellum nonhuman priority journal trypanosoma cruzi Animal Binding Sites Brain Ca(2+)-Calmodulin Dependent Protein Kinase Calmodulin Cattle Cell Fractionation Cell Membrane Comparative Study Cytoskeleton Flagella Microscopy, Electron Support, Non-U.S. Gov't Trypanosoma cruzi Trypanosoma cruzi epimastigote forms showed a tightly bound Ca2+-calmodulin-dependent protein kinase activity, which could be partially extracted from membranes and axonemes. The enzyme is constituted by subunits which were autophosphorylated in the absence of exogenous substrates. An antibody against CaM kinase II recognized a Ca2+- or Ca2+-CaM-dependent conformational epitope in these fractions. The detected bands were of molecular weights similar to the α and β subunits of the corresponding bovine brain enzyme (60 and 50 kDa). Studies using [125I]CaM revealed the presence of a CaM-binding domain. These experiments confirm that the parasite possesses a paniculate CaM kinase with characteristics similar to the bovine brain enzyme. © 1994. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v337_n3_p293_Ogueta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Ca2+-calmodulin protein kinase Cytoskeleton Flagella Trypanosoma cruzi Trypanosomatid protein kinase (calcium,calmodulin) ii article cytoskeleton epimastigote flagellum nonhuman priority journal trypanosoma cruzi Animal Binding Sites Brain Ca(2+)-Calmodulin Dependent Protein Kinase Calmodulin Cattle Cell Fractionation Cell Membrane Comparative Study Cytoskeleton Flagella Microscopy, Electron Support, Non-U.S. Gov't Trypanosoma cruzi |
| spellingShingle |
Ca2+-calmodulin protein kinase Cytoskeleton Flagella Trypanosoma cruzi Trypanosomatid protein kinase (calcium,calmodulin) ii article cytoskeleton epimastigote flagellum nonhuman priority journal trypanosoma cruzi Animal Binding Sites Brain Ca(2+)-Calmodulin Dependent Protein Kinase Calmodulin Cattle Cell Fractionation Cell Membrane Comparative Study Cytoskeleton Flagella Microscopy, Electron Support, Non-U.S. Gov't Trypanosoma cruzi Ogueta, S.B. Solari, A. Téllez-Iñón, M.T. Trypanosoma cruzi epimastigote forms possess a Ca2+-calmodulin dependent protein kinase |
| topic_facet |
Ca2+-calmodulin protein kinase Cytoskeleton Flagella Trypanosoma cruzi Trypanosomatid protein kinase (calcium,calmodulin) ii article cytoskeleton epimastigote flagellum nonhuman priority journal trypanosoma cruzi Animal Binding Sites Brain Ca(2+)-Calmodulin Dependent Protein Kinase Calmodulin Cattle Cell Fractionation Cell Membrane Comparative Study Cytoskeleton Flagella Microscopy, Electron Support, Non-U.S. Gov't Trypanosoma cruzi |
| description |
Trypanosoma cruzi epimastigote forms showed a tightly bound Ca2+-calmodulin-dependent protein kinase activity, which could be partially extracted from membranes and axonemes. The enzyme is constituted by subunits which were autophosphorylated in the absence of exogenous substrates. An antibody against CaM kinase II recognized a Ca2+- or Ca2+-CaM-dependent conformational epitope in these fractions. The detected bands were of molecular weights similar to the α and β subunits of the corresponding bovine brain enzyme (60 and 50 kDa). Studies using [125I]CaM revealed the presence of a CaM-binding domain. These experiments confirm that the parasite possesses a paniculate CaM kinase with characteristics similar to the bovine brain enzyme. © 1994. |
| format |
JOUR |
| author |
Ogueta, S.B. Solari, A. Téllez-Iñón, M.T. |
| author_facet |
Ogueta, S.B. Solari, A. Téllez-Iñón, M.T. |
| author_sort |
Ogueta, S.B. |
| title |
Trypanosoma cruzi epimastigote forms possess a Ca2+-calmodulin dependent protein kinase |
| title_short |
Trypanosoma cruzi epimastigote forms possess a Ca2+-calmodulin dependent protein kinase |
| title_full |
Trypanosoma cruzi epimastigote forms possess a Ca2+-calmodulin dependent protein kinase |
| title_fullStr |
Trypanosoma cruzi epimastigote forms possess a Ca2+-calmodulin dependent protein kinase |
| title_full_unstemmed |
Trypanosoma cruzi epimastigote forms possess a Ca2+-calmodulin dependent protein kinase |
| title_sort |
trypanosoma cruzi epimastigote forms possess a ca2+-calmodulin dependent protein kinase |
| url |
http://hdl.handle.net/20.500.12110/paper_00145793_v337_n3_p293_Ogueta |
| work_keys_str_mv |
AT oguetasb trypanosomacruziepimastigoteformspossessaca2calmodulindependentproteinkinase AT solaria trypanosomacruziepimastigoteformspossessaca2calmodulindependentproteinkinase AT tellezinonmt trypanosomacruziepimastigoteformspossessaca2calmodulindependentproteinkinase |
| _version_ |
1782031067036778496 |