Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans

GTP stimulation of adenylyl cyclase from the dimorphic pathogenic fungus Candida albicans is greatly enhanced by preincubation of membrane proteins with cholera toxin, NAD and ATP. In the presence of [32P]NAD the toxin catalyzes the covalent incorporation of radioactivity into a menbrane protein of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Paveto, C., Montero, L., Passeron, S.
Formato:	JOUR
Materias:	Adenylyl cyclase Candida albicans G-protein Hormone response Peptide antiserum, α-commons adenosine triphosphate adenylate cyclase cholera toxin guanine nucleotide binding protein membrane protein nicotinamide adenine dinucleotide pertussis toxin protein subunit article candida albicans nonhuman priority journal signal transduction Adenosine Triphosphate Adenylate Cyclase Blotting, Western Cholera Toxin GTP-Binding Proteins Guanosine Triphosphate Membrane Proteins NAD Poly(ADP-ribose) Polymerases Support, Non-U.S. Gov't Virulence Factors, Bordetella
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00145793_v311_n1_p51_Paveto
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00145793_v311_n1_p51_Paveto
record_format	dspace
spelling	todo:paper_00145793_v311_n1_p51_Paveto2023-10-03T14:13:02Z Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans Paveto, C. Montero, L. Passeron, S. Adenylyl cyclase Candida albicans G-protein Hormone response Peptide antiserum, α-commons adenosine triphosphate adenylate cyclase cholera toxin guanine nucleotide binding protein membrane protein nicotinamide adenine dinucleotide pertussis toxin protein subunit article candida albicans nonhuman priority journal signal transduction Adenosine Triphosphate Adenylate Cyclase Blotting, Western Candida albicans Cholera Toxin GTP-Binding Proteins Guanosine Triphosphate Membrane Proteins NAD Poly(ADP-ribose) Polymerases Support, Non-U.S. Gov't Virulence Factors, Bordetella GTP stimulation of adenylyl cyclase from the dimorphic pathogenic fungus Candida albicans is greatly enhanced by preincubation of membrane proteins with cholera toxin, NAD and ATP. In the presence of [32P]NAD the toxin catalyzes the covalent incorporation of radioactivity into a menbrane protein of 40 kDa. Pertussis toxin catalyzes the transference of the radioactivity from [32P]NAD to a 32 kDa protein. Two major proteins of 40-42 and 30-32 kDa can also be recognized in Western blots by an anti Gα-common antibody. The results support the idea that G proteins are part of the hormonesensory transduction chain of Candida [(1990) Biochem. Biophys. Res. Commun. 167. 1177-1183]. © 1992. Fil:Paveto, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Montero, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v311_n1_p51_Paveto
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Adenylyl cyclase Candida albicans G-protein Hormone response Peptide antiserum, α-commons adenosine triphosphate adenylate cyclase cholera toxin guanine nucleotide binding protein membrane protein nicotinamide adenine dinucleotide pertussis toxin protein subunit article candida albicans nonhuman priority journal signal transduction Adenosine Triphosphate Adenylate Cyclase Blotting, Western Candida albicans Cholera Toxin GTP-Binding Proteins Guanosine Triphosphate Membrane Proteins NAD Poly(ADP-ribose) Polymerases Support, Non-U.S. Gov't Virulence Factors, Bordetella
spellingShingle	Adenylyl cyclase Candida albicans G-protein Hormone response Peptide antiserum, α-commons adenosine triphosphate adenylate cyclase cholera toxin guanine nucleotide binding protein membrane protein nicotinamide adenine dinucleotide pertussis toxin protein subunit article candida albicans nonhuman priority journal signal transduction Adenosine Triphosphate Adenylate Cyclase Blotting, Western Candida albicans Cholera Toxin GTP-Binding Proteins Guanosine Triphosphate Membrane Proteins NAD Poly(ADP-ribose) Polymerases Support, Non-U.S. Gov't Virulence Factors, Bordetella Paveto, C. Montero, L. Passeron, S. Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans
topic_facet	Adenylyl cyclase Candida albicans G-protein Hormone response Peptide antiserum, α-commons adenosine triphosphate adenylate cyclase cholera toxin guanine nucleotide binding protein membrane protein nicotinamide adenine dinucleotide pertussis toxin protein subunit article candida albicans nonhuman priority journal signal transduction Adenosine Triphosphate Adenylate Cyclase Blotting, Western Candida albicans Cholera Toxin GTP-Binding Proteins Guanosine Triphosphate Membrane Proteins NAD Poly(ADP-ribose) Polymerases Support, Non-U.S. Gov't Virulence Factors, Bordetella
description	GTP stimulation of adenylyl cyclase from the dimorphic pathogenic fungus Candida albicans is greatly enhanced by preincubation of membrane proteins with cholera toxin, NAD and ATP. In the presence of [32P]NAD the toxin catalyzes the covalent incorporation of radioactivity into a menbrane protein of 40 kDa. Pertussis toxin catalyzes the transference of the radioactivity from [32P]NAD to a 32 kDa protein. Two major proteins of 40-42 and 30-32 kDa can also be recognized in Western blots by an anti Gα-common antibody. The results support the idea that G proteins are part of the hormonesensory transduction chain of Candida [(1990) Biochem. Biophys. Res. Commun. 167. 1177-1183]. © 1992.
format	JOUR
author	Paveto, C. Montero, L. Passeron, S.
author_facet	Paveto, C. Montero, L. Passeron, S.
author_sort	Paveto, C.
title	Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans
title_short	Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans
title_full	Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans
title_fullStr	Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans
title_full_unstemmed	Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans
title_sort	enzymatic and immunological detection of g protein α-subunits in the pathogenic fungus candida albicans
url	http://hdl.handle.net/20.500.12110/paper_00145793_v311_n1_p51_Paveto
work_keys_str_mv	AT pavetoc enzymaticandimmunologicaldetectionofgproteinasubunitsinthepathogenicfunguscandidaalbicans AT monterol enzymaticandimmunologicaldetectionofgproteinasubunitsinthepathogenicfunguscandidaalbicans AT passerons enzymaticandimmunologicaldetectionofgproteinasubunitsinthepathogenicfunguscandidaalbicans
_version_	1807324226953150464

Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans

Ejemplares similares