Stimulation of protein kinase C (PKC) activity in resting Swiss 3T3 cells by prostaglandin F2α
Prostaglandin F2α (PGF2α), a mitogen for resting Swiss 3T3 cells, rapidly stimulates phosphorylation of an 80 kDa protein (80 K). 1-Oleoyl-2-acetylglycerol (OAG) and 12-O-tetradecanoyl phorbol-13-acetate (TPA) both protein kinase C (PKC) activators, also elicit 80 K phosphorylation. In contrast PGE1...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00145793_v297_n1-2_p175_Goin |
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todo:paper_00145793_v297_n1-2_p175_Goin2023-10-03T14:13:00Z Stimulation of protein kinase C (PKC) activity in resting Swiss 3T3 cells by prostaglandin F2α Goin, M. de Asua, L.J. 80 K phosphorylation DNA synthesis Prostaglandin F2α Protein kinase C Swiss 3T3 cell prostaglandin e1 prostaglandin e2 prostaglandin f2 alpha protein kinase c animal cell article enzyme activation mouse nonhuman priority journal 3T3 Cells Alprostadil Animal Cell Division Diglycerides Dinoprost Dinoprostone DNA Electrophoresis, Polyacrylamide Gel Enzyme Activation Mice Phosphorylation Protein Kinase C Support, Non-U.S. Gov't Tetradecanoylphorbol Acetate Prostaglandin F2α (PGF2α), a mitogen for resting Swiss 3T3 cells, rapidly stimulates phosphorylation of an 80 kDa protein (80 K). 1-Oleoyl-2-acetylglycerol (OAG) and 12-O-tetradecanoyl phorbol-13-acetate (TPA) both protein kinase C (PKC) activators, also elicit 80 K phosphorylation. In contrast PGE1, PGE2 or PGF2β, which are non-mitogenic in these cells, had tittle or no action on this event. However PGE1 and PGE2 potentiate the PGF2α proliferative effect but do not enhance its action on 80 K phosphorylation. These results suggest that PGF2α mitogenic induction involves PKC signalling pathway activation while its enhancement by PGE1 or PGE2 occurs through a different mechanism(s). © 1992. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v297_n1-2_p175_Goin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
80 K phosphorylation DNA synthesis Prostaglandin F2α Protein kinase C Swiss 3T3 cell prostaglandin e1 prostaglandin e2 prostaglandin f2 alpha protein kinase c animal cell article enzyme activation mouse nonhuman priority journal 3T3 Cells Alprostadil Animal Cell Division Diglycerides Dinoprost Dinoprostone DNA Electrophoresis, Polyacrylamide Gel Enzyme Activation Mice Phosphorylation Protein Kinase C Support, Non-U.S. Gov't Tetradecanoylphorbol Acetate |
| spellingShingle |
80 K phosphorylation DNA synthesis Prostaglandin F2α Protein kinase C Swiss 3T3 cell prostaglandin e1 prostaglandin e2 prostaglandin f2 alpha protein kinase c animal cell article enzyme activation mouse nonhuman priority journal 3T3 Cells Alprostadil Animal Cell Division Diglycerides Dinoprost Dinoprostone DNA Electrophoresis, Polyacrylamide Gel Enzyme Activation Mice Phosphorylation Protein Kinase C Support, Non-U.S. Gov't Tetradecanoylphorbol Acetate Goin, M. de Asua, L.J. Stimulation of protein kinase C (PKC) activity in resting Swiss 3T3 cells by prostaglandin F2α |
| topic_facet |
80 K phosphorylation DNA synthesis Prostaglandin F2α Protein kinase C Swiss 3T3 cell prostaglandin e1 prostaglandin e2 prostaglandin f2 alpha protein kinase c animal cell article enzyme activation mouse nonhuman priority journal 3T3 Cells Alprostadil Animal Cell Division Diglycerides Dinoprost Dinoprostone DNA Electrophoresis, Polyacrylamide Gel Enzyme Activation Mice Phosphorylation Protein Kinase C Support, Non-U.S. Gov't Tetradecanoylphorbol Acetate |
| description |
Prostaglandin F2α (PGF2α), a mitogen for resting Swiss 3T3 cells, rapidly stimulates phosphorylation of an 80 kDa protein (80 K). 1-Oleoyl-2-acetylglycerol (OAG) and 12-O-tetradecanoyl phorbol-13-acetate (TPA) both protein kinase C (PKC) activators, also elicit 80 K phosphorylation. In contrast PGE1, PGE2 or PGF2β, which are non-mitogenic in these cells, had tittle or no action on this event. However PGE1 and PGE2 potentiate the PGF2α proliferative effect but do not enhance its action on 80 K phosphorylation. These results suggest that PGF2α mitogenic induction involves PKC signalling pathway activation while its enhancement by PGE1 or PGE2 occurs through a different mechanism(s). © 1992. |
| format |
JOUR |
| author |
Goin, M. de Asua, L.J. |
| author_facet |
Goin, M. de Asua, L.J. |
| author_sort |
Goin, M. |
| title |
Stimulation of protein kinase C (PKC) activity in resting Swiss 3T3 cells by prostaglandin F2α |
| title_short |
Stimulation of protein kinase C (PKC) activity in resting Swiss 3T3 cells by prostaglandin F2α |
| title_full |
Stimulation of protein kinase C (PKC) activity in resting Swiss 3T3 cells by prostaglandin F2α |
| title_fullStr |
Stimulation of protein kinase C (PKC) activity in resting Swiss 3T3 cells by prostaglandin F2α |
| title_full_unstemmed |
Stimulation of protein kinase C (PKC) activity in resting Swiss 3T3 cells by prostaglandin F2α |
| title_sort |
stimulation of protein kinase c (pkc) activity in resting swiss 3t3 cells by prostaglandin f2α |
| url |
http://hdl.handle.net/20.500.12110/paper_00145793_v297_n1-2_p175_Goin |
| work_keys_str_mv |
AT goinm stimulationofproteinkinasecpkcactivityinrestingswiss3t3cellsbyprostaglandinf2a AT deasualj stimulationofproteinkinasecpkcactivityinrestingswiss3t3cellsbyprostaglandinf2a |
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