Tunicamycin inhibits the initiation of DNA synthesis stimulated by prostaglandin F2α in Swiss mouse 3T3 cells
Tunicamycin, an inhibitor of the asparagine-linked protein N-glycosylation, blocks the initiation of DNA synthesis in Swiss 3T3 cells stimulated by prostaglandin F2α alone or with insulin. This effect is exerted only when tunicamycin is added from 0 to 8 h after stimulation and it decreases the rate...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00145793_v290_n1-2_p239_Estevez |
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todo:paper_00145793_v290_n1-2_p239_Estevez2023-10-03T14:13:00Z Tunicamycin inhibits the initiation of DNA synthesis stimulated by prostaglandin F2α in Swiss mouse 3T3 cells Estevez, A.G. Calamante, G. Goin, M. de Asua, L.J. DNA synthesis Lag phase Prostaglandin F2α Swiss 3T3 cell Tunicamycin insulin prostaglandin f2 alpha tunicamycin animal cell article cell growth dna synthesis mouse nonhuman priority journal 3T3 Cells Animal Cell Division Dinoprost DNA Glucosamine Glycoproteins In Vitro Insulin Mannose Mice Support, Non-U.S. Gov't Time Factors Tunicamycin Animalia Tunicamycin, an inhibitor of the asparagine-linked protein N-glycosylation, blocks the initiation of DNA synthesis in Swiss 3T3 cells stimulated by prostaglandin F2α alone or with insulin. This effect is exerted only when tunicamycin is added from 0 to 8 h after stimulation and it decreases the rate of entry into S phase. Blocking of labeled sugar incorporation to proteins occurs regardless of the time of PGF2α stimulation. In contrast tunicamicin does not inhibit protein synthesis. These results suggest that N-glycoprotein synthesis early during the prereplicative phase is an important event controlling the mitogenic action of PGF2α. © 1991. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v290_n1-2_p239_Estevez |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
DNA synthesis Lag phase Prostaglandin F2α Swiss 3T3 cell Tunicamycin insulin prostaglandin f2 alpha tunicamycin animal cell article cell growth dna synthesis mouse nonhuman priority journal 3T3 Cells Animal Cell Division Dinoprost DNA Glucosamine Glycoproteins In Vitro Insulin Mannose Mice Support, Non-U.S. Gov't Time Factors Tunicamycin Animalia |
| spellingShingle |
DNA synthesis Lag phase Prostaglandin F2α Swiss 3T3 cell Tunicamycin insulin prostaglandin f2 alpha tunicamycin animal cell article cell growth dna synthesis mouse nonhuman priority journal 3T3 Cells Animal Cell Division Dinoprost DNA Glucosamine Glycoproteins In Vitro Insulin Mannose Mice Support, Non-U.S. Gov't Time Factors Tunicamycin Animalia Estevez, A.G. Calamante, G. Goin, M. de Asua, L.J. Tunicamycin inhibits the initiation of DNA synthesis stimulated by prostaglandin F2α in Swiss mouse 3T3 cells |
| topic_facet |
DNA synthesis Lag phase Prostaglandin F2α Swiss 3T3 cell Tunicamycin insulin prostaglandin f2 alpha tunicamycin animal cell article cell growth dna synthesis mouse nonhuman priority journal 3T3 Cells Animal Cell Division Dinoprost DNA Glucosamine Glycoproteins In Vitro Insulin Mannose Mice Support, Non-U.S. Gov't Time Factors Tunicamycin Animalia |
| description |
Tunicamycin, an inhibitor of the asparagine-linked protein N-glycosylation, blocks the initiation of DNA synthesis in Swiss 3T3 cells stimulated by prostaglandin F2α alone or with insulin. This effect is exerted only when tunicamycin is added from 0 to 8 h after stimulation and it decreases the rate of entry into S phase. Blocking of labeled sugar incorporation to proteins occurs regardless of the time of PGF2α stimulation. In contrast tunicamicin does not inhibit protein synthesis. These results suggest that N-glycoprotein synthesis early during the prereplicative phase is an important event controlling the mitogenic action of PGF2α. © 1991. |
| format |
JOUR |
| author |
Estevez, A.G. Calamante, G. Goin, M. de Asua, L.J. |
| author_facet |
Estevez, A.G. Calamante, G. Goin, M. de Asua, L.J. |
| author_sort |
Estevez, A.G. |
| title |
Tunicamycin inhibits the initiation of DNA synthesis stimulated by prostaglandin F2α in Swiss mouse 3T3 cells |
| title_short |
Tunicamycin inhibits the initiation of DNA synthesis stimulated by prostaglandin F2α in Swiss mouse 3T3 cells |
| title_full |
Tunicamycin inhibits the initiation of DNA synthesis stimulated by prostaglandin F2α in Swiss mouse 3T3 cells |
| title_fullStr |
Tunicamycin inhibits the initiation of DNA synthesis stimulated by prostaglandin F2α in Swiss mouse 3T3 cells |
| title_full_unstemmed |
Tunicamycin inhibits the initiation of DNA synthesis stimulated by prostaglandin F2α in Swiss mouse 3T3 cells |
| title_sort |
tunicamycin inhibits the initiation of dna synthesis stimulated by prostaglandin f2α in swiss mouse 3t3 cells |
| url |
http://hdl.handle.net/20.500.12110/paper_00145793_v290_n1-2_p239_Estevez |
| work_keys_str_mv |
AT estevezag tunicamycininhibitstheinitiationofdnasynthesisstimulatedbyprostaglandinf2ainswissmouse3t3cells AT calamanteg tunicamycininhibitstheinitiationofdnasynthesisstimulatedbyprostaglandinf2ainswissmouse3t3cells AT goinm tunicamycininhibitstheinitiationofdnasynthesisstimulatedbyprostaglandinf2ainswissmouse3t3cells AT deasualj tunicamycininhibitstheinitiationofdnasynthesisstimulatedbyprostaglandinf2ainswissmouse3t3cells |
| _version_ |
1807320771572269056 |