Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization

Monoclonal antibodies to Neurospora crassa cyclic nucleotide phosphodiesterase (PDE I) were selected by their capacity to inhibit the enzyme activity. The monoclonal immunoglobulin, coupled to Sepharose 4B, was used for the affinity purification of PDE I activity. After SDS-polyacrylamide gel electr...

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Detalles Bibliográficos
Autores principales: Ulloa, R.M., Rubinstein, C.P., Molina y Vedia, L., Torres, H.N., Téllez-Iñón, M.T.
Formato: JOUR
Materias:
(Neurospora crassa)
Cyclic AMP phosphodiesterase
Immunoaffinity chromatography
Monoclonal antibody
cyclic amp phosphodiesterase
monoclonal antibody
enzyme purification
fungus
immunoaffinity chromatography
neurospora crassa
nonhuman
priority journal
3',5'-Cyclic-Nucleotide Phosphodiesterase
Antibodies, Monoclonal
Chromatography, Affinity
Immunoglobulins, Heavy-Chain
Immunoglobulins, Light-Chain
Kinetics
Molecular Weight
Neurospora
Neurospora crassa
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00145793_v241_n1-2_p219_Ulloa
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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