Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization

Monoclonal antibodies to Neurospora crassa cyclic nucleotide phosphodiesterase (PDE I) were selected by their capacity to inhibit the enzyme activity. The monoclonal immunoglobulin, coupled to Sepharose 4B, was used for the affinity purification of PDE I activity. After SDS-polyacrylamide gel electr...

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Autores principales:	Ulloa, R.M., Rubinstein, C.P., Molina y Vedia, L., Torres, H.N., Téllez-Iñón, M.T.
Formato:	JOUR
Materias:	(Neurospora crassa) Cyclic AMP phosphodiesterase Immunoaffinity chromatography Monoclonal antibody cyclic amp phosphodiesterase monoclonal antibody enzyme purification fungus immunoaffinity chromatography neurospora crassa nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Antibodies, Monoclonal Chromatography, Affinity Immunoglobulins, Heavy-Chain Immunoglobulins, Light-Chain Kinetics Molecular Weight Neurospora Neurospora crassa
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00145793_v241_n1-2_p219_Ulloa
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00145793_v241_n1-2_p219_Ulloa2023-10-03T14:12:58Z Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization Ulloa, R.M. Rubinstein, C.P. Molina y Vedia, L. Torres, H.N. Téllez-Iñón, M.T. (Neurospora crassa) Cyclic AMP phosphodiesterase Immunoaffinity chromatography Monoclonal antibody cyclic amp phosphodiesterase monoclonal antibody enzyme purification fungus immunoaffinity chromatography neurospora crassa nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Antibodies, Monoclonal Chromatography, Affinity Immunoglobulins, Heavy-Chain Immunoglobulins, Light-Chain Kinetics Molecular Weight Neurospora Neurospora crassa Monoclonal antibodies to Neurospora crassa cyclic nucleotide phosphodiesterase (PDE I) were selected by their capacity to inhibit the enzyme activity. The monoclonal immunoglobulin, coupled to Sepharose 4B, was used for the affinity purification of PDE I activity. After SDS-polyacrylamide gel electrophoresis the affinity purified PDE I fractions showed a single polypeptide band of about 41 kDa. This band reacted in Western blots with the above mentioned monoclonal immunoglobulin. © 1988. Fil:Rubinstein, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Molina y Vedia, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v241_n1-2_p219_Ulloa
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	(Neurospora crassa) Cyclic AMP phosphodiesterase Immunoaffinity chromatography Monoclonal antibody cyclic amp phosphodiesterase monoclonal antibody enzyme purification fungus immunoaffinity chromatography neurospora crassa nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Antibodies, Monoclonal Chromatography, Affinity Immunoglobulins, Heavy-Chain Immunoglobulins, Light-Chain Kinetics Molecular Weight Neurospora Neurospora crassa
spellingShingle	(Neurospora crassa) Cyclic AMP phosphodiesterase Immunoaffinity chromatography Monoclonal antibody cyclic amp phosphodiesterase monoclonal antibody enzyme purification fungus immunoaffinity chromatography neurospora crassa nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Antibodies, Monoclonal Chromatography, Affinity Immunoglobulins, Heavy-Chain Immunoglobulins, Light-Chain Kinetics Molecular Weight Neurospora Neurospora crassa Ulloa, R.M. Rubinstein, C.P. Molina y Vedia, L. Torres, H.N. Téllez-Iñón, M.T. Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization
topic_facet	(Neurospora crassa) Cyclic AMP phosphodiesterase Immunoaffinity chromatography Monoclonal antibody cyclic amp phosphodiesterase monoclonal antibody enzyme purification fungus immunoaffinity chromatography neurospora crassa nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Antibodies, Monoclonal Chromatography, Affinity Immunoglobulins, Heavy-Chain Immunoglobulins, Light-Chain Kinetics Molecular Weight Neurospora Neurospora crassa
description	Monoclonal antibodies to Neurospora crassa cyclic nucleotide phosphodiesterase (PDE I) were selected by their capacity to inhibit the enzyme activity. The monoclonal immunoglobulin, coupled to Sepharose 4B, was used for the affinity purification of PDE I activity. After SDS-polyacrylamide gel electrophoresis the affinity purified PDE I fractions showed a single polypeptide band of about 41 kDa. This band reacted in Western blots with the above mentioned monoclonal immunoglobulin. © 1988.
format	JOUR
author	Ulloa, R.M. Rubinstein, C.P. Molina y Vedia, L. Torres, H.N. Téllez-Iñón, M.T.
author_facet	Ulloa, R.M. Rubinstein, C.P. Molina y Vedia, L. Torres, H.N. Téllez-Iñón, M.T.
author_sort	Ulloa, R.M.
title	Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization
title_short	Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization
title_full	Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization
title_fullStr	Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization
title_full_unstemmed	Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization
title_sort	cyclic nucleotide phosphodiesterase activity in neurospora crassa purification by immunoaffinity chromatography and characterization
url	http://hdl.handle.net/20.500.12110/paper_00145793_v241_n1-2_p219_Ulloa
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Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization

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