Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin

The effect of colchicine, vinblastine, and cytochalasin B has been investigated on the phytohemagglutinin (PHA) induced transformation and DNA synthesis of human lymphocytes. The three drugs produced, at an appropriate concentration, inhibition of DNA synthesis and lymphocyte transformation, if adde...

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Detalles Bibliográficos
Autores principales: Medrano, E., Piras, R., Mordoh, J.
Formato: JOUR
Materias:
colchicine
cytochalasin b
phytohemagglutinin
sepharose
vinblastine
dna synthesis
human
in vitro study
lymphocyte
lymphocyte membrane
lymphocyte transformation
normal human
theoretical study
thymidine h 3
Binding Sites
Cell Division
Cell Survival
Colchicine
Cytochalasin B
DNA
Human
In Vitro
Lectins
Lymphocyte Activation
Lymphocytes
Thymidine
Time Factors
Tritium
Vinblastine
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00144827_v86_n2_p295_Medrano
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The effect of colchicine, vinblastine, and cytochalasin B has been investigated on the phytohemagglutinin (PHA) induced transformation and DNA synthesis of human lymphocytes. The three drugs produced, at an appropriate concentration, inhibition of DNA synthesis and lymphocyte transformation, if added prior to PHA. Inhibitory concentrations of cytochalasin B were no longer effective in preventing DNA synthesis if added 2 h after exposure to PHA; on the other hand, colchicine and vinblastine were effective even if they were added 16 h after PHA. Studies of lymphocyte aggregation to beads of Sepharose with chemically bound PHA suggest that the effects of these drugs do not seem to lie primarily on blocking PHA binding to the lymphocyte membrane, but rather on a subsequent step(s). © 1974.

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