Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin
The effect of colchicine, vinblastine, and cytochalasin B has been investigated on the phytohemagglutinin (PHA) induced transformation and DNA synthesis of human lymphocytes. The three drugs produced, at an appropriate concentration, inhibition of DNA synthesis and lymphocyte transformation, if adde...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00144827_v86_n2_p295_Medrano |
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todo:paper_00144827_v86_n2_p295_Medrano2023-10-03T14:12:41Z Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin Medrano, E. Piras, R. Mordoh, J. colchicine cytochalasin b phytohemagglutinin sepharose vinblastine dna synthesis human in vitro study lymphocyte lymphocyte membrane lymphocyte transformation normal human theoretical study thymidine h 3 Binding Sites Cell Division Cell Survival Colchicine Cytochalasin B DNA Human In Vitro Lectins Lymphocyte Activation Lymphocytes Thymidine Time Factors Tritium Vinblastine The effect of colchicine, vinblastine, and cytochalasin B has been investigated on the phytohemagglutinin (PHA) induced transformation and DNA synthesis of human lymphocytes. The three drugs produced, at an appropriate concentration, inhibition of DNA synthesis and lymphocyte transformation, if added prior to PHA. Inhibitory concentrations of cytochalasin B were no longer effective in preventing DNA synthesis if added 2 h after exposure to PHA; on the other hand, colchicine and vinblastine were effective even if they were added 16 h after PHA. Studies of lymphocyte aggregation to beads of Sepharose with chemically bound PHA suggest that the effects of these drugs do not seem to lie primarily on blocking PHA binding to the lymphocyte membrane, but rather on a subsequent step(s). © 1974. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00144827_v86_n2_p295_Medrano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
colchicine cytochalasin b phytohemagglutinin sepharose vinblastine dna synthesis human in vitro study lymphocyte lymphocyte membrane lymphocyte transformation normal human theoretical study thymidine h 3 Binding Sites Cell Division Cell Survival Colchicine Cytochalasin B DNA Human In Vitro Lectins Lymphocyte Activation Lymphocytes Thymidine Time Factors Tritium Vinblastine |
| spellingShingle |
colchicine cytochalasin b phytohemagglutinin sepharose vinblastine dna synthesis human in vitro study lymphocyte lymphocyte membrane lymphocyte transformation normal human theoretical study thymidine h 3 Binding Sites Cell Division Cell Survival Colchicine Cytochalasin B DNA Human In Vitro Lectins Lymphocyte Activation Lymphocytes Thymidine Time Factors Tritium Vinblastine Medrano, E. Piras, R. Mordoh, J. Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin |
| topic_facet |
colchicine cytochalasin b phytohemagglutinin sepharose vinblastine dna synthesis human in vitro study lymphocyte lymphocyte membrane lymphocyte transformation normal human theoretical study thymidine h 3 Binding Sites Cell Division Cell Survival Colchicine Cytochalasin B DNA Human In Vitro Lectins Lymphocyte Activation Lymphocytes Thymidine Time Factors Tritium Vinblastine |
| description |
The effect of colchicine, vinblastine, and cytochalasin B has been investigated on the phytohemagglutinin (PHA) induced transformation and DNA synthesis of human lymphocytes. The three drugs produced, at an appropriate concentration, inhibition of DNA synthesis and lymphocyte transformation, if added prior to PHA. Inhibitory concentrations of cytochalasin B were no longer effective in preventing DNA synthesis if added 2 h after exposure to PHA; on the other hand, colchicine and vinblastine were effective even if they were added 16 h after PHA. Studies of lymphocyte aggregation to beads of Sepharose with chemically bound PHA suggest that the effects of these drugs do not seem to lie primarily on blocking PHA binding to the lymphocyte membrane, but rather on a subsequent step(s). © 1974. |
| format |
JOUR |
| author |
Medrano, E. Piras, R. Mordoh, J. |
| author_facet |
Medrano, E. Piras, R. Mordoh, J. |
| author_sort |
Medrano, E. |
| title |
Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin |
| title_short |
Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin |
| title_full |
Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin |
| title_fullStr |
Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin |
| title_full_unstemmed |
Effect of colchicine, vinblastine and cytochalasin B on human lymphocyte transformation by phytohemagglutinin |
| title_sort |
effect of colchicine, vinblastine and cytochalasin b on human lymphocyte transformation by phytohemagglutinin |
| url |
http://hdl.handle.net/20.500.12110/paper_00144827_v86_n2_p295_Medrano |
| work_keys_str_mv |
AT medranoe effectofcolchicinevinblastineandcytochalasinbonhumanlymphocytetransformationbyphytohemagglutinin AT pirasr effectofcolchicinevinblastineandcytochalasinbonhumanlymphocytetransformationbyphytohemagglutinin AT mordohj effectofcolchicinevinblastineandcytochalasinbonhumanlymphocytetransformationbyphytohemagglutinin |
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