Increased secretory activity and estradiol receptor expression are among other relevant aspects of MCF-7 human breast tumor cell growth which are expressed only in the absence of serum

We compared the morphology, clonogenic ability, Percoll gradient distribution, estrogen receptor proteins, and interactions with mesenchymal cells in MCF-7 breast tumor cells grown in medium containing fetal calf serum and insulin (FCS-I) or in a defined medium with insulin (ID) as the only growth f...

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Autores principales:	Medrano, E.E., Resnicoff, M., Cafferata, E.G.A., Larcher, F., Podhajcer, O., Bover, L., Molinari, B.
Formato:	JOUR
Materias:	Mink cell focus-forming virus estradiol receptor article breast tumor cell culture cell growth gene expression human human cell priority journal protein secretion tumor cell Animal Breast Neoplasms Cell Transformation, Neoplastic Culture Media Embryo Epithelium Female Fibroblasts Human Immunohistochemistry Insulin Methionine Microscopy, Electron Proteins Rats Receptors, Estradiol Serum Albumin, Bovine Sulfur Radioisotopes Support, Non-U.S. Gov't Tumor Cells, Cultured
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00144827_v188_n1_p2_Medrano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00144827_v188_n1_p2_Medrano2023-10-03T14:12:37Z Increased secretory activity and estradiol receptor expression are among other relevant aspects of MCF-7 human breast tumor cell growth which are expressed only in the absence of serum Medrano, E.E. Resnicoff, M. Cafferata, E.G.A. Larcher, F. Podhajcer, O. Bover, L. Molinari, B. Mink cell focus-forming virus Mink cell focus-forming virus estradiol receptor article breast tumor cell culture cell growth gene expression human human cell priority journal protein secretion tumor cell Animal Breast Neoplasms Cell Transformation, Neoplastic Culture Media Embryo Epithelium Female Fibroblasts Human Immunohistochemistry Insulin Methionine Microscopy, Electron Proteins Rats Receptors, Estradiol Serum Albumin, Bovine Sulfur Radioisotopes Support, Non-U.S. Gov't Tumor Cells, Cultured We compared the morphology, clonogenic ability, Percoll gradient distribution, estrogen receptor proteins, and interactions with mesenchymal cells in MCF-7 breast tumor cells grown in medium containing fetal calf serum and insulin (FCS-I) or in a defined medium with insulin (ID) as the only growth factor. In the absence of serum and at densities below 5000-8000 cells/cm2, MCF-7 cells required epidermal growth factor, insulin, and thrombin. When cells reached a density of 23,000-26,000 cells/cm2, only insulin was necessary for optimal growth. In ID medium cells showed an enlarged Golgi apparatus and marked plasma membrane modifications, suggesting increased secretory activity. Moreover there was an increase in the release of protein products to the culture medium and a time-dependent ability of these cells to form macrocolonies in soft agar. On the contrary, cells in FCS-I showed no Golgi complex and few plasma membrane modifications. In both culture media tight junctions, desmosomes, and tonofilaments were present. We investigated the effect of conditioned media from MCF-7 cells growing in FCS-I or ID on the growth of primary rat vaginal fibroblasts. The growth of these mesenchymal cells was stimulated by FCS-I medium and inhibited by ID medium. By contrast, the embryonic fibroblast (preadipocyte) line CHEF/18 was also stimulated by FCS-I for the first 48 h, but thereafter ceased growth and acquired lipid droplets and a differentiated morphology. With ID medium, CHEF/18 cells were only partially inhibited with no changes in morphology. The Percoll gradient profiles of ID cells showed the same six fractions of increasing density as recently described. However, there was a progressive increase in subpopulations with higher growth rates and a decrease in the relative amount of the most differentiated cells. A unique feature of the growth analysis of MCF-7 cells in the absence of serum is the increased expression of the estradiol receptor gene. These studies show that the growth and differentiated properties of tumor cells can depend upon the cellular environment and offer a model system in which to further study this modulation. © 1990. Fil:Medrano, E.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Resnicoff, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Podhajcer, O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bover, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Molinari, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00144827_v188_n1_p2_Medrano
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Mink cell focus-forming virus Mink cell focus-forming virus estradiol receptor article breast tumor cell culture cell growth gene expression human human cell priority journal protein secretion tumor cell Animal Breast Neoplasms Cell Transformation, Neoplastic Culture Media Embryo Epithelium Female Fibroblasts Human Immunohistochemistry Insulin Methionine Microscopy, Electron Proteins Rats Receptors, Estradiol Serum Albumin, Bovine Sulfur Radioisotopes Support, Non-U.S. Gov't Tumor Cells, Cultured
spellingShingle	Mink cell focus-forming virus Mink cell focus-forming virus estradiol receptor article breast tumor cell culture cell growth gene expression human human cell priority journal protein secretion tumor cell Animal Breast Neoplasms Cell Transformation, Neoplastic Culture Media Embryo Epithelium Female Fibroblasts Human Immunohistochemistry Insulin Methionine Microscopy, Electron Proteins Rats Receptors, Estradiol Serum Albumin, Bovine Sulfur Radioisotopes Support, Non-U.S. Gov't Tumor Cells, Cultured Medrano, E.E. Resnicoff, M. Cafferata, E.G.A. Larcher, F. Podhajcer, O. Bover, L. Molinari, B. Increased secretory activity and estradiol receptor expression are among other relevant aspects of MCF-7 human breast tumor cell growth which are expressed only in the absence of serum
topic_facet	Mink cell focus-forming virus Mink cell focus-forming virus estradiol receptor article breast tumor cell culture cell growth gene expression human human cell priority journal protein secretion tumor cell Animal Breast Neoplasms Cell Transformation, Neoplastic Culture Media Embryo Epithelium Female Fibroblasts Human Immunohistochemistry Insulin Methionine Microscopy, Electron Proteins Rats Receptors, Estradiol Serum Albumin, Bovine Sulfur Radioisotopes Support, Non-U.S. Gov't Tumor Cells, Cultured
description	We compared the morphology, clonogenic ability, Percoll gradient distribution, estrogen receptor proteins, and interactions with mesenchymal cells in MCF-7 breast tumor cells grown in medium containing fetal calf serum and insulin (FCS-I) or in a defined medium with insulin (ID) as the only growth factor. In the absence of serum and at densities below 5000-8000 cells/cm2, MCF-7 cells required epidermal growth factor, insulin, and thrombin. When cells reached a density of 23,000-26,000 cells/cm2, only insulin was necessary for optimal growth. In ID medium cells showed an enlarged Golgi apparatus and marked plasma membrane modifications, suggesting increased secretory activity. Moreover there was an increase in the release of protein products to the culture medium and a time-dependent ability of these cells to form macrocolonies in soft agar. On the contrary, cells in FCS-I showed no Golgi complex and few plasma membrane modifications. In both culture media tight junctions, desmosomes, and tonofilaments were present. We investigated the effect of conditioned media from MCF-7 cells growing in FCS-I or ID on the growth of primary rat vaginal fibroblasts. The growth of these mesenchymal cells was stimulated by FCS-I medium and inhibited by ID medium. By contrast, the embryonic fibroblast (preadipocyte) line CHEF/18 was also stimulated by FCS-I for the first 48 h, but thereafter ceased growth and acquired lipid droplets and a differentiated morphology. With ID medium, CHEF/18 cells were only partially inhibited with no changes in morphology. The Percoll gradient profiles of ID cells showed the same six fractions of increasing density as recently described. However, there was a progressive increase in subpopulations with higher growth rates and a decrease in the relative amount of the most differentiated cells. A unique feature of the growth analysis of MCF-7 cells in the absence of serum is the increased expression of the estradiol receptor gene. These studies show that the growth and differentiated properties of tumor cells can depend upon the cellular environment and offer a model system in which to further study this modulation. © 1990.
format	JOUR
author	Medrano, E.E. Resnicoff, M. Cafferata, E.G.A. Larcher, F. Podhajcer, O. Bover, L. Molinari, B.
author_facet	Medrano, E.E. Resnicoff, M. Cafferata, E.G.A. Larcher, F. Podhajcer, O. Bover, L. Molinari, B.
author_sort	Medrano, E.E.
title	Increased secretory activity and estradiol receptor expression are among other relevant aspects of MCF-7 human breast tumor cell growth which are expressed only in the absence of serum
title_short	Increased secretory activity and estradiol receptor expression are among other relevant aspects of MCF-7 human breast tumor cell growth which are expressed only in the absence of serum
title_full	Increased secretory activity and estradiol receptor expression are among other relevant aspects of MCF-7 human breast tumor cell growth which are expressed only in the absence of serum
title_fullStr	Increased secretory activity and estradiol receptor expression are among other relevant aspects of MCF-7 human breast tumor cell growth which are expressed only in the absence of serum
title_full_unstemmed	Increased secretory activity and estradiol receptor expression are among other relevant aspects of MCF-7 human breast tumor cell growth which are expressed only in the absence of serum
title_sort	increased secretory activity and estradiol receptor expression are among other relevant aspects of mcf-7 human breast tumor cell growth which are expressed only in the absence of serum
url	http://hdl.handle.net/20.500.12110/paper_00144827_v188_n1_p2_Medrano
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Increased secretory activity and estradiol receptor expression are among other relevant aspects of MCF-7 human breast tumor cell growth which are expressed only in the absence of serum

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