Transfer of oligosaccharide from oligosaccharide pyrophosphoryl dolichol to endogenous acceptor proteins in human breast malignant and normal tissues

We have prepared dolichylpyrophosphoryl-[14C]-oligosaccharide (Dol-PP-oligosaccharide) from calf thyroid. Microsomal fractions from human breast tissues catalyzed the transfer of labeled oligosaccharide to endogenous acceptor proteins. Malignant tumors showed higher activity of the oligosaccharide t...

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Detalles Bibliográficos
Autores principales: Santa Cruz, A.R., Baldi, A.
Formato: JOUR
Materias:
human breast cancer glycoproteins
kojibiose
Oligosaccharide transfer
glycoprotein
radioisotope
animal cell
breast
breast cancer
cattle
cow
diagnosis
endocrine system
human
human cell
nonhuman
oligosaccharide transfer
priority journal
thyroid gland
Animal
Breast
Breast Neoplasms
Cattle
Disaccharides
Female
Glycoproteins
Human
Microsomes
Oligosaccharides
Polyisoprenyl Phosphate Sugars
Protein Processing, Post-Translational
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00144754_v41_n7_p928_SantaCruz
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:We have prepared dolichylpyrophosphoryl-[14C]-oligosaccharide (Dol-PP-oligosaccharide) from calf thyroid. Microsomal fractions from human breast tissues catalyzed the transfer of labeled oligosaccharide to endogenous acceptor proteins. Malignant tumors showed higher activity of the oligosaccharide transferring enzyme than normal tissue. With kojibiose (Kj), and inhibitor of (Glc3)-glucosidase, an increase in the radioactivity associated with glycoprotein was observed. © 1985 Birkhäuser Verlag.

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