Inhibitory effects of GABA, L-glutamic acid and nicotine on the potassium-evoked release of substance P in substantia nigra slices of the rat

Rat substantia nigra slices were superfused with a physiological medium containing a diluted substance P (SP) antiserum, bacitracin and serum albumin to measure SP released in superfusate. As shown by measuring the degradation of a SP-labelled derivative incubated with cerebellar slices, the medium...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Borda, E., Del Carmen Agostini, M., Sterin-Borda, L., Gimeno, M.F., Gimeno, A.L.
Formato: JOUR
Materias:
4 aminobutyric acid
glutamic acid
glutamic acid diethyl ester
glycine
nicotine
oxotremorine
pempidine
picrotoxin
potassium
serotonin
substance P
tetrodotoxin
veratridine
animal experiment
brain slice
central nervous system
controlled study
drug comparison
in vitro study
rat
substantia nigra
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00142999_v71_n4_p383_Borda
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00142999_v71_n4_p383_Borda
record_format dspace
spelling todo:paper_00142999_v71_n4_p383_Borda2023-10-03T14:12:11Z Inhibitory effects of GABA, L-glutamic acid and nicotine on the potassium-evoked release of substance P in substantia nigra slices of the rat Borda, E. Del Carmen Agostini, M. Sterin-Borda, L. Gimeno, M.F. Gimeno, A.L. 4 aminobutyric acid glutamic acid glutamic acid diethyl ester glycine nicotine oxotremorine pempidine picrotoxin potassium serotonin substance P tetrodotoxin veratridine animal experiment brain slice central nervous system controlled study drug comparison in vitro study rat substantia nigra Rat substantia nigra slices were superfused with a physiological medium containing a diluted substance P (SP) antiserum, bacitracin and serum albumin to measure SP released in superfusate. As shown by measuring the degradation of a SP-labelled derivative incubated with cerebellar slices, the medium prevented the enzymatic inactivation of SP. Potassium (K+, 50 mM) and veratridine (5 x 10-5 M) stimulated SP release and these effects were respectively prevented in absence of calcium and in presence of tetrodotoxin ( 5 x 10-7 M). GABA (5 x 10-5 M), nicotine (5 x 10-6 M) and L-glutamic acid (5 x 10-5 M) reduced the K+ (50 mM)-evoked release of SP. In contrast, glycine (5 x 10-5 M), oxotremorine (5 x 10-5 M), D-glutamic acid (5 x 10-5 M) and serotonin (5 x 10-5 M) were without effect. Pempidine (5 x 10-5 M) prevented the inhibitory effect of nicotine (5 x 10-6 M) on the K+-evoked release of SP. Glutamic acid diethyl ester (10-4 M) completely abolished the L-glutamic acid-induced inhibition of the K+-evoked release of SP. Picrotoxin (5 x 10-5 M) did not influence the L-glutamic acid inhibitory effect excluding the intervention of GABAergic mechanisms. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142999_v71_n4_p383_Borda
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 4 aminobutyric acid
glutamic acid
glutamic acid diethyl ester
glycine
nicotine
oxotremorine
pempidine
picrotoxin
potassium
serotonin
substance P
tetrodotoxin
veratridine
animal experiment
brain slice
central nervous system
controlled study
drug comparison
in vitro study
rat
substantia nigra
spellingShingle 4 aminobutyric acid
glutamic acid
glutamic acid diethyl ester
glycine
nicotine
oxotremorine
pempidine
picrotoxin
potassium
serotonin
substance P
tetrodotoxin
veratridine
animal experiment
brain slice
central nervous system
controlled study
drug comparison
in vitro study
rat
substantia nigra
Borda, E.
Del Carmen Agostini, M.
Sterin-Borda, L.
Gimeno, M.F.
Gimeno, A.L.
Inhibitory effects of GABA, L-glutamic acid and nicotine on the potassium-evoked release of substance P in substantia nigra slices of the rat
topic_facet 4 aminobutyric acid
glutamic acid
glutamic acid diethyl ester
glycine
nicotine
oxotremorine
pempidine
picrotoxin
potassium
serotonin
substance P
tetrodotoxin
veratridine
animal experiment
brain slice
central nervous system
controlled study
drug comparison
in vitro study
rat
substantia nigra
description Rat substantia nigra slices were superfused with a physiological medium containing a diluted substance P (SP) antiserum, bacitracin and serum albumin to measure SP released in superfusate. As shown by measuring the degradation of a SP-labelled derivative incubated with cerebellar slices, the medium prevented the enzymatic inactivation of SP. Potassium (K+, 50 mM) and veratridine (5 x 10-5 M) stimulated SP release and these effects were respectively prevented in absence of calcium and in presence of tetrodotoxin ( 5 x 10-7 M). GABA (5 x 10-5 M), nicotine (5 x 10-6 M) and L-glutamic acid (5 x 10-5 M) reduced the K+ (50 mM)-evoked release of SP. In contrast, glycine (5 x 10-5 M), oxotremorine (5 x 10-5 M), D-glutamic acid (5 x 10-5 M) and serotonin (5 x 10-5 M) were without effect. Pempidine (5 x 10-5 M) prevented the inhibitory effect of nicotine (5 x 10-6 M) on the K+-evoked release of SP. Glutamic acid diethyl ester (10-4 M) completely abolished the L-glutamic acid-induced inhibition of the K+-evoked release of SP. Picrotoxin (5 x 10-5 M) did not influence the L-glutamic acid inhibitory effect excluding the intervention of GABAergic mechanisms.
format JOUR
author Borda, E.
Del Carmen Agostini, M.
Sterin-Borda, L.
Gimeno, M.F.
Gimeno, A.L.
author_facet Borda, E.
Del Carmen Agostini, M.
Sterin-Borda, L.
Gimeno, M.F.
Gimeno, A.L.
author_sort Borda, E.
title Inhibitory effects of GABA, L-glutamic acid and nicotine on the potassium-evoked release of substance P in substantia nigra slices of the rat
title_short Inhibitory effects of GABA, L-glutamic acid and nicotine on the potassium-evoked release of substance P in substantia nigra slices of the rat
title_full Inhibitory effects of GABA, L-glutamic acid and nicotine on the potassium-evoked release of substance P in substantia nigra slices of the rat
title_fullStr Inhibitory effects of GABA, L-glutamic acid and nicotine on the potassium-evoked release of substance P in substantia nigra slices of the rat
title_full_unstemmed Inhibitory effects of GABA, L-glutamic acid and nicotine on the potassium-evoked release of substance P in substantia nigra slices of the rat
title_sort inhibitory effects of gaba, l-glutamic acid and nicotine on the potassium-evoked release of substance p in substantia nigra slices of the rat
url http://hdl.handle.net/20.500.12110/paper_00142999_v71_n4_p383_Borda
work_keys_str_mv AT bordae inhibitoryeffectsofgabalglutamicacidandnicotineonthepotassiumevokedreleaseofsubstancepinsubstantianigraslicesoftherat
AT delcarmenagostinim inhibitoryeffectsofgabalglutamicacidandnicotineonthepotassiumevokedreleaseofsubstancepinsubstantianigraslicesoftherat
AT sterinbordal inhibitoryeffectsofgabalglutamicacidandnicotineonthepotassiumevokedreleaseofsubstancepinsubstantianigraslicesoftherat
AT gimenomf inhibitoryeffectsofgabalglutamicacidandnicotineonthepotassiumevokedreleaseofsubstancepinsubstantianigraslicesoftherat
AT gimenoal inhibitoryeffectsofgabalglutamicacidandnicotineonthepotassiumevokedreleaseofsubstancepinsubstantianigraslicesoftherat
_version_ 1782024433882365952

Ejemplares similares