The positive inotropic effect of sodium arachidonate on auricles from diabetic rats
Contractile responses to exogenous sodium arachidonate (NaA) were studied in auricles from normal and acutely diabetic (streptozotocin-treated) rats. NaA induced a concentration-dependent positive inotropic effect in atria from normal and diabetic rats but the magnitude of the contractile influence...
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todo:paper_00142999_v110_n1_p47_Canga2023-10-03T14:12:03Z The positive inotropic effect of sodium arachidonate on auricles from diabetic rats Canga, L. Sterin-Borda, L. Borda, E.S. Peredo, H. Gimeno, A.L. Arachidonic acid Atria Cyclo-oxygenase Diabetes Lipoxygenase(s) Prostacyclin Streptozotocin Thromboxane acetylsalicylic acid arachidonic acid arachidonic acid c 14 dithizone imidazole indometacin lipoxygenase nictindole nordihydroguaiaretic acid prostaglandin synthase prostaglandin synthase inhibitor radioisotope streptozocin tranylcypromine unclassified drug animal cell animal experiment animal model article diabetes mellitus drug efficacy drug interaction endocrine system heart heart atrium inotropism nonhuman priority journal rat Animals Arachidonic Acid Arachidonic Acids Carbon Radioisotopes Cyclooxygenase Inhibitors Diabetes Mellitus, Experimental Epoprostenol Heart Atria Lipoxygenase Inhibitors Male Myocardial Contraction Rats Rats, Inbred Strains Stimulation, Chemical Thromboxane-A Synthase Contractile responses to exogenous sodium arachidonate (NaA) were studied in auricles from normal and acutely diabetic (streptozotocin-treated) rats. NaA induced a concentration-dependent positive inotropic effect in atria from normal and diabetic rats but the magnitude of the contractile influence of the fatty acid was different in both types of auricles, i.e. diabetic atria had a greater resonsiveness to NaA than normal preparations. Blockers of arachidonic acid (AA) metabolism via cyclo-oxygenase (indomethacin and acetylsalicylic acid), abolished the stimulatory influence of NaA in normal and in diabetic auricles. Moreover, incubation with tranylcipromine, a prostacyclin synthetase inhibitor, reduced the inotropic effect of NaA in normal atria, but failed to modify it in the diabetic atria. On the other hand, inhibitors of thromboxane (TX) synthetase (imidazole and L-8027) diminished the effect of NaA in diabetic preparations without altering the contractile responses in the controls. In the presence of lipoxygenase blocking agents (nordihydroguaiaretic acid and dithizone), the influence of NaA was reduced at normal values only in the atria from diabetic rats. Both atrial preparations (normal and diabetic) converted ([1-14C]arachidonic acid (AA) into PGI2 (assessed as 6-oxo-PGF1α) and TXB2. Moreover, normal atria generated more PGI2 than TXB2 whereas TXB2 production was greater than that of PGI2 in diabetic atria. The foregoing results suggest that in non-diabetic atria the effect of exogenous NaA may be attributed to metabolites generated by the sequential action of cyclo-oxygenase and prostacyclin synthetase. On the other hand, the contractile action of exogenous NaA in diabetic atria may be ascribed to thromboxanes and lipoxygenase(s) metabolites. A possible modulatory influence of TX on the action of lipoxygenase products is postulated. © 1985. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142999_v110_n1_p47_Canga |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arachidonic acid Atria Cyclo-oxygenase Diabetes Lipoxygenase(s) Prostacyclin Streptozotocin Thromboxane acetylsalicylic acid arachidonic acid arachidonic acid c 14 dithizone imidazole indometacin lipoxygenase nictindole nordihydroguaiaretic acid prostaglandin synthase prostaglandin synthase inhibitor radioisotope streptozocin tranylcypromine unclassified drug animal cell animal experiment animal model article diabetes mellitus drug efficacy drug interaction endocrine system heart heart atrium inotropism nonhuman priority journal rat Animals Arachidonic Acid Arachidonic Acids Carbon Radioisotopes Cyclooxygenase Inhibitors Diabetes Mellitus, Experimental Epoprostenol Heart Atria Lipoxygenase Inhibitors Male Myocardial Contraction Rats Rats, Inbred Strains Stimulation, Chemical Thromboxane-A Synthase |
| spellingShingle |
Arachidonic acid Atria Cyclo-oxygenase Diabetes Lipoxygenase(s) Prostacyclin Streptozotocin Thromboxane acetylsalicylic acid arachidonic acid arachidonic acid c 14 dithizone imidazole indometacin lipoxygenase nictindole nordihydroguaiaretic acid prostaglandin synthase prostaglandin synthase inhibitor radioisotope streptozocin tranylcypromine unclassified drug animal cell animal experiment animal model article diabetes mellitus drug efficacy drug interaction endocrine system heart heart atrium inotropism nonhuman priority journal rat Animals Arachidonic Acid Arachidonic Acids Carbon Radioisotopes Cyclooxygenase Inhibitors Diabetes Mellitus, Experimental Epoprostenol Heart Atria Lipoxygenase Inhibitors Male Myocardial Contraction Rats Rats, Inbred Strains Stimulation, Chemical Thromboxane-A Synthase Canga, L. Sterin-Borda, L. Borda, E.S. Peredo, H. Gimeno, A.L. The positive inotropic effect of sodium arachidonate on auricles from diabetic rats |
| topic_facet |
Arachidonic acid Atria Cyclo-oxygenase Diabetes Lipoxygenase(s) Prostacyclin Streptozotocin Thromboxane acetylsalicylic acid arachidonic acid arachidonic acid c 14 dithizone imidazole indometacin lipoxygenase nictindole nordihydroguaiaretic acid prostaglandin synthase prostaglandin synthase inhibitor radioisotope streptozocin tranylcypromine unclassified drug animal cell animal experiment animal model article diabetes mellitus drug efficacy drug interaction endocrine system heart heart atrium inotropism nonhuman priority journal rat Animals Arachidonic Acid Arachidonic Acids Carbon Radioisotopes Cyclooxygenase Inhibitors Diabetes Mellitus, Experimental Epoprostenol Heart Atria Lipoxygenase Inhibitors Male Myocardial Contraction Rats Rats, Inbred Strains Stimulation, Chemical Thromboxane-A Synthase |
| description |
Contractile responses to exogenous sodium arachidonate (NaA) were studied in auricles from normal and acutely diabetic (streptozotocin-treated) rats. NaA induced a concentration-dependent positive inotropic effect in atria from normal and diabetic rats but the magnitude of the contractile influence of the fatty acid was different in both types of auricles, i.e. diabetic atria had a greater resonsiveness to NaA than normal preparations. Blockers of arachidonic acid (AA) metabolism via cyclo-oxygenase (indomethacin and acetylsalicylic acid), abolished the stimulatory influence of NaA in normal and in diabetic auricles. Moreover, incubation with tranylcipromine, a prostacyclin synthetase inhibitor, reduced the inotropic effect of NaA in normal atria, but failed to modify it in the diabetic atria. On the other hand, inhibitors of thromboxane (TX) synthetase (imidazole and L-8027) diminished the effect of NaA in diabetic preparations without altering the contractile responses in the controls. In the presence of lipoxygenase blocking agents (nordihydroguaiaretic acid and dithizone), the influence of NaA was reduced at normal values only in the atria from diabetic rats. Both atrial preparations (normal and diabetic) converted ([1-14C]arachidonic acid (AA) into PGI2 (assessed as 6-oxo-PGF1α) and TXB2. Moreover, normal atria generated more PGI2 than TXB2 whereas TXB2 production was greater than that of PGI2 in diabetic atria. The foregoing results suggest that in non-diabetic atria the effect of exogenous NaA may be attributed to metabolites generated by the sequential action of cyclo-oxygenase and prostacyclin synthetase. On the other hand, the contractile action of exogenous NaA in diabetic atria may be ascribed to thromboxanes and lipoxygenase(s) metabolites. A possible modulatory influence of TX on the action of lipoxygenase products is postulated. © 1985. |
| format |
JOUR |
| author |
Canga, L. Sterin-Borda, L. Borda, E.S. Peredo, H. Gimeno, A.L. |
| author_facet |
Canga, L. Sterin-Borda, L. Borda, E.S. Peredo, H. Gimeno, A.L. |
| author_sort |
Canga, L. |
| title |
The positive inotropic effect of sodium arachidonate on auricles from diabetic rats |
| title_short |
The positive inotropic effect of sodium arachidonate on auricles from diabetic rats |
| title_full |
The positive inotropic effect of sodium arachidonate on auricles from diabetic rats |
| title_fullStr |
The positive inotropic effect of sodium arachidonate on auricles from diabetic rats |
| title_full_unstemmed |
The positive inotropic effect of sodium arachidonate on auricles from diabetic rats |
| title_sort |
positive inotropic effect of sodium arachidonate on auricles from diabetic rats |
| url |
http://hdl.handle.net/20.500.12110/paper_00142999_v110_n1_p47_Canga |
| work_keys_str_mv |
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| _version_ |
1782024870175965184 |