Lipid‐Bound Oligosaccharides in Insects

Membrane preparations from immature stages of the fruit fly Ceratitis capitata catalyze the transfer of mannose from GDP‐[14C]mannose into lipid‐linked oligosaccharides. These compounds behave as polyprenyl derivatives and their formation is stimulated by the addition of an acidic glyco‐lipid fracti...

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Autores principales:	QUESADA ALLUE, L.A., BELOCOPITOW, E.
Formato:	JOUR
Materias:	glycolipid guanosine diphosphate mannose mannose animal experiment arthropod in vitro study Animal Diptera Guanosine Diphosphate Mannose Kinetics Oligosaccharides Polyethylene Glycols Polyisoprenyl Phosphate Oligosaccharides Polyisoprenyl Phosphate Sugars
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00142956_v88_n2_p529_QUESADAALLUE
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00142956_v88_n2_p529_QUESADAALLUE
record_format	dspace
spelling	todo:paper_00142956_v88_n2_p529_QUESADAALLUE2023-10-03T14:11:59Z Lipid‐Bound Oligosaccharides in Insects QUESADA ALLUE, L.A. BELOCOPITOW, E. glycolipid guanosine diphosphate mannose mannose animal experiment arthropod in vitro study Animal Diptera Guanosine Diphosphate Mannose Kinetics Oligosaccharides Polyethylene Glycols Polyisoprenyl Phosphate Oligosaccharides Polyisoprenyl Phosphate Sugars Membrane preparations from immature stages of the fruit fly Ceratitis capitata catalyze the transfer of mannose from GDP‐[14C]mannose into lipid‐linked oligosaccharides. These compounds behave as polyprenyl derivatives and their formation is stimulated by the addition of an acidic glyco‐lipid fraction isolated from insects. The mannose‐labeled oligosaccharides are attached to the poly‐isoprenol by a pyrophosphoryl linkage and can be released by mild acid hydrolysis. The trisaccharide lipid has been partially characterized. The results indicate that the compound is polyprenyl‐pyrophosphate‐N,N′‐diacetylchitobiose‐mannose. Incubation of dolichyl phosphate [14C]mannose or lower 14C‐labeled oligosaccharide lipids with unlabeled GDP‐mannose and the insect enzyme leads to the labeling of a higher lipid‐bound oligosaccharide. When UDP‐N‐acetyl[14C]glucosamine was incubated with insect membranes a 14C‐labeled chitobiosyl lipid was synthesized. If unlabeled GDP‐mannose was also present, the 14C label appeared in the trisaccharide and higher oligosaccharide lipids. Preliminary evidence indicates that the insect polyprenyl oligosaccharides described here might participate in glycoprotein biosynthesis. Copyright © 1978, Wiley Blackwell. All rights reserved JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142956_v88_n2_p529_QUESADAALLUE
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	glycolipid guanosine diphosphate mannose mannose animal experiment arthropod in vitro study Animal Diptera Guanosine Diphosphate Mannose Kinetics Oligosaccharides Polyethylene Glycols Polyisoprenyl Phosphate Oligosaccharides Polyisoprenyl Phosphate Sugars
spellingShingle	glycolipid guanosine diphosphate mannose mannose animal experiment arthropod in vitro study Animal Diptera Guanosine Diphosphate Mannose Kinetics Oligosaccharides Polyethylene Glycols Polyisoprenyl Phosphate Oligosaccharides Polyisoprenyl Phosphate Sugars QUESADA ALLUE, L.A. BELOCOPITOW, E. Lipid‐Bound Oligosaccharides in Insects
topic_facet	glycolipid guanosine diphosphate mannose mannose animal experiment arthropod in vitro study Animal Diptera Guanosine Diphosphate Mannose Kinetics Oligosaccharides Polyethylene Glycols Polyisoprenyl Phosphate Oligosaccharides Polyisoprenyl Phosphate Sugars
description	Membrane preparations from immature stages of the fruit fly Ceratitis capitata catalyze the transfer of mannose from GDP‐[14C]mannose into lipid‐linked oligosaccharides. These compounds behave as polyprenyl derivatives and their formation is stimulated by the addition of an acidic glyco‐lipid fraction isolated from insects. The mannose‐labeled oligosaccharides are attached to the poly‐isoprenol by a pyrophosphoryl linkage and can be released by mild acid hydrolysis. The trisaccharide lipid has been partially characterized. The results indicate that the compound is polyprenyl‐pyrophosphate‐N,N′‐diacetylchitobiose‐mannose. Incubation of dolichyl phosphate [14C]mannose or lower 14C‐labeled oligosaccharide lipids with unlabeled GDP‐mannose and the insect enzyme leads to the labeling of a higher lipid‐bound oligosaccharide. When UDP‐N‐acetyl[14C]glucosamine was incubated with insect membranes a 14C‐labeled chitobiosyl lipid was synthesized. If unlabeled GDP‐mannose was also present, the 14C label appeared in the trisaccharide and higher oligosaccharide lipids. Preliminary evidence indicates that the insect polyprenyl oligosaccharides described here might participate in glycoprotein biosynthesis. Copyright © 1978, Wiley Blackwell. All rights reserved
format	JOUR
author	QUESADA ALLUE, L.A. BELOCOPITOW, E.
author_facet	QUESADA ALLUE, L.A. BELOCOPITOW, E.
author_sort	QUESADA ALLUE, L.A.
title	Lipid‐Bound Oligosaccharides in Insects
title_short	Lipid‐Bound Oligosaccharides in Insects
title_full	Lipid‐Bound Oligosaccharides in Insects
title_fullStr	Lipid‐Bound Oligosaccharides in Insects
title_full_unstemmed	Lipid‐Bound Oligosaccharides in Insects
title_sort	lipid‐bound oligosaccharides in insects
url	http://hdl.handle.net/20.500.12110/paper_00142956_v88_n2_p529_QUESADAALLUE
work_keys_str_mv	AT quesadaalluela lipidboundoligosaccharidesininsects AT belocopitowe lipidboundoligosaccharidesininsects
_version_	1782028177120428032

Lipid‐Bound Oligosaccharides in Insects

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