Polypeptide Synthesis in Toluene‐Treated Lymphocytes
Normal human lymphocytes stimulated by phytohemagglutinin P, and other mammalian cells were rendered permeable to macromolecules such as poly(U) and proteins, by treatment with a low concentration of toluene. Under this condition, poly(U) translation was more efficient in the permeabilized cells tha...
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todo:paper_00142956_v86_n2_p439_BURRONE2023-10-03T14:11:59Z Polypeptide Synthesis in Toluene‐Treated Lymphocytes BURRONE, O.R. adenosine diphosphate adenosine triphosphate creatine kinase creatine phosphate guanosine triphosphate nuclease phenylalanine phytohemagglutinin p polypeptide polyuridylic acid radioisotope toluene in vitro study lymphocyte membrane permeability pharmacokinetics phenylalanine c 14 protein synthesis staphylococcus aureus uridine c 14 Animal Carcinoma, Ehrlich Tumor Comparative Study Human Lymphocytes Magnesium Peptide Synthesis Phenylalanine Poly U Ribonucleases Ribosomes Staphylococcus aureus Toluene Uridine Normal human lymphocytes stimulated by phytohemagglutinin P, and other mammalian cells were rendered permeable to macromolecules such as poly(U) and proteins, by treatment with a low concentration of toluene. Under this condition, poly(U) translation was more efficient in the permeabilized cells than in 10000 ×g extracts. Such a process occurs inside the treated cells as demonstrated by the fact that [14C]uridine‐labelled ribosomes remain associated with the toluene‐treated lymphocytes even after incubation at 37 °C. A nuclease from Staphylococcus aureus was able to penetrate the permeabilized cells and to break the polysome‐bound endogenous messenger RNA. However, the protein‐synthesizing machinery inside the toluene‐treated lymphocytes was unaffected by the nuclease, as demonstrated by the unimpairement of polyphenylalanine synthesis when poly(U) was added after the preincubation with the enzyme. These results suggest that the toluene treatment can be considered as an important tool for the study of the synthesis of macromolecules and its regulation in eukaryotic cells. Copyright © 1978, Wiley Blackwell. All rights reserved JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142956_v86_n2_p439_BURRONE |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adenosine diphosphate adenosine triphosphate creatine kinase creatine phosphate guanosine triphosphate nuclease phenylalanine phytohemagglutinin p polypeptide polyuridylic acid radioisotope toluene in vitro study lymphocyte membrane permeability pharmacokinetics phenylalanine c 14 protein synthesis staphylococcus aureus uridine c 14 Animal Carcinoma, Ehrlich Tumor Comparative Study Human Lymphocytes Magnesium Peptide Synthesis Phenylalanine Poly U Ribonucleases Ribosomes Staphylococcus aureus Toluene Uridine |
| spellingShingle |
adenosine diphosphate adenosine triphosphate creatine kinase creatine phosphate guanosine triphosphate nuclease phenylalanine phytohemagglutinin p polypeptide polyuridylic acid radioisotope toluene in vitro study lymphocyte membrane permeability pharmacokinetics phenylalanine c 14 protein synthesis staphylococcus aureus uridine c 14 Animal Carcinoma, Ehrlich Tumor Comparative Study Human Lymphocytes Magnesium Peptide Synthesis Phenylalanine Poly U Ribonucleases Ribosomes Staphylococcus aureus Toluene Uridine BURRONE, O.R. Polypeptide Synthesis in Toluene‐Treated Lymphocytes |
| topic_facet |
adenosine diphosphate adenosine triphosphate creatine kinase creatine phosphate guanosine triphosphate nuclease phenylalanine phytohemagglutinin p polypeptide polyuridylic acid radioisotope toluene in vitro study lymphocyte membrane permeability pharmacokinetics phenylalanine c 14 protein synthesis staphylococcus aureus uridine c 14 Animal Carcinoma, Ehrlich Tumor Comparative Study Human Lymphocytes Magnesium Peptide Synthesis Phenylalanine Poly U Ribonucleases Ribosomes Staphylococcus aureus Toluene Uridine |
| description |
Normal human lymphocytes stimulated by phytohemagglutinin P, and other mammalian cells were rendered permeable to macromolecules such as poly(U) and proteins, by treatment with a low concentration of toluene. Under this condition, poly(U) translation was more efficient in the permeabilized cells than in 10000 ×g extracts. Such a process occurs inside the treated cells as demonstrated by the fact that [14C]uridine‐labelled ribosomes remain associated with the toluene‐treated lymphocytes even after incubation at 37 °C. A nuclease from Staphylococcus aureus was able to penetrate the permeabilized cells and to break the polysome‐bound endogenous messenger RNA. However, the protein‐synthesizing machinery inside the toluene‐treated lymphocytes was unaffected by the nuclease, as demonstrated by the unimpairement of polyphenylalanine synthesis when poly(U) was added after the preincubation with the enzyme. These results suggest that the toluene treatment can be considered as an important tool for the study of the synthesis of macromolecules and its regulation in eukaryotic cells. Copyright © 1978, Wiley Blackwell. All rights reserved |
| format |
JOUR |
| author |
BURRONE, O.R. |
| author_facet |
BURRONE, O.R. |
| author_sort |
BURRONE, O.R. |
| title |
Polypeptide Synthesis in Toluene‐Treated Lymphocytes |
| title_short |
Polypeptide Synthesis in Toluene‐Treated Lymphocytes |
| title_full |
Polypeptide Synthesis in Toluene‐Treated Lymphocytes |
| title_fullStr |
Polypeptide Synthesis in Toluene‐Treated Lymphocytes |
| title_full_unstemmed |
Polypeptide Synthesis in Toluene‐Treated Lymphocytes |
| title_sort |
polypeptide synthesis in toluene‐treated lymphocytes |
| url |
http://hdl.handle.net/20.500.12110/paper_00142956_v86_n2_p439_BURRONE |
| work_keys_str_mv |
AT burroneor polypeptidesynthesisintoluenetreatedlymphocytes |
| _version_ |
1782029007139635200 |