Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars
Particulate preparations from the Chlorophyta Prototheca zopfii catalyze the incorporation of [14C]glucose from UDP‐[14C]glucose into lipids. These lipids have been characterized as lipid‐P‐glucose, lipid‐PP‐glucose, and lipid‐PP‐oligosaccharides. The lipid‐linked oligosaccharides were a mixture ran...
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todo:paper_00142956_v84_n2_p561_HOPP2023-10-03T14:11:58Z Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars HOPP, H.E. ROMERO, P.A. DALEO, G.R. PONT LEZICA, R. cellulose radioisotope animal experiment cell wall in vitro study Algae, Green Cellulose Dolichol Glycolipids Guanosine Diphosphate Sugars Oligosaccharides Polyisoprenyl Phosphates Temperature Uridine Diphosphate Uridine Diphosphate Glucose Uridine Monophosphate Particulate preparations from the Chlorophyta Prototheca zopfii catalyze the incorporation of [14C]glucose from UDP‐[14C]glucose into lipids. These lipids have been characterized as lipid‐P‐glucose, lipid‐PP‐glucose, and lipid‐PP‐oligosaccharides. The lipid‐linked oligosaccharides were a mixture ranging from a disaccharide to approximately a decasaccharide. Cellulase digestion and periodate oxidation showed that the oligosaccharides seem to be built of β‐1,4‐linked glucoses. The lipid moiety had the properties of dolichol. The glucolipids described appeared as precursors of a water‐soluble polymer. Treatments of this polymer with hydrolytic enzymes and periodate oxidation indicate that it could be a glycoprotein containing β‐1,4‐linked glucoses. When GDP‐glucose was added to the incubation mixture, the 14C‐labelled soluble polymer became insoluble in hot alkali. This insoluble polymer had the properties expected for cellulose. A scheme is proposed with the reactions involved in the initiation of cellulose biosynthesis. Copyright © 1978, Wiley Blackwell. All rights reserved JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142956_v84_n2_p561_HOPP |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cellulose radioisotope animal experiment cell wall in vitro study Algae, Green Cellulose Dolichol Glycolipids Guanosine Diphosphate Sugars Oligosaccharides Polyisoprenyl Phosphates Temperature Uridine Diphosphate Uridine Diphosphate Glucose Uridine Monophosphate |
| spellingShingle |
cellulose radioisotope animal experiment cell wall in vitro study Algae, Green Cellulose Dolichol Glycolipids Guanosine Diphosphate Sugars Oligosaccharides Polyisoprenyl Phosphates Temperature Uridine Diphosphate Uridine Diphosphate Glucose Uridine Monophosphate HOPP, H.E. ROMERO, P.A. DALEO, G.R. PONT LEZICA, R. Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars |
| topic_facet |
cellulose radioisotope animal experiment cell wall in vitro study Algae, Green Cellulose Dolichol Glycolipids Guanosine Diphosphate Sugars Oligosaccharides Polyisoprenyl Phosphates Temperature Uridine Diphosphate Uridine Diphosphate Glucose Uridine Monophosphate |
| description |
Particulate preparations from the Chlorophyta Prototheca zopfii catalyze the incorporation of [14C]glucose from UDP‐[14C]glucose into lipids. These lipids have been characterized as lipid‐P‐glucose, lipid‐PP‐glucose, and lipid‐PP‐oligosaccharides. The lipid‐linked oligosaccharides were a mixture ranging from a disaccharide to approximately a decasaccharide. Cellulase digestion and periodate oxidation showed that the oligosaccharides seem to be built of β‐1,4‐linked glucoses. The lipid moiety had the properties of dolichol. The glucolipids described appeared as precursors of a water‐soluble polymer. Treatments of this polymer with hydrolytic enzymes and periodate oxidation indicate that it could be a glycoprotein containing β‐1,4‐linked glucoses. When GDP‐glucose was added to the incubation mixture, the 14C‐labelled soluble polymer became insoluble in hot alkali. This insoluble polymer had the properties expected for cellulose. A scheme is proposed with the reactions involved in the initiation of cellulose biosynthesis. Copyright © 1978, Wiley Blackwell. All rights reserved |
| format |
JOUR |
| author |
HOPP, H.E. ROMERO, P.A. DALEO, G.R. PONT LEZICA, R. |
| author_facet |
HOPP, H.E. ROMERO, P.A. DALEO, G.R. PONT LEZICA, R. |
| author_sort |
HOPP, H.E. |
| title |
Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars |
| title_short |
Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars |
| title_full |
Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars |
| title_fullStr |
Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars |
| title_full_unstemmed |
Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars |
| title_sort |
synthesis of cellulose precursors: the involvement of lipid‐linked sugars |
| url |
http://hdl.handle.net/20.500.12110/paper_00142956_v84_n2_p561_HOPP |
| work_keys_str_mv |
AT hopphe synthesisofcelluloseprecursorstheinvolvementoflipidlinkedsugars AT romeropa synthesisofcelluloseprecursorstheinvolvementoflipidlinkedsugars AT daleogr synthesisofcelluloseprecursorstheinvolvementoflipidlinkedsugars AT pontlezicar synthesisofcelluloseprecursorstheinvolvementoflipidlinkedsugars |
| _version_ |
1782027706630668288 |