α‐Glucan synthesis on a protein primer: A reconstituted system for the formation of protein‐bound α‐glucan
Reconstitution experiments with the DEAE‐cellulose‐treated enzymes, engaged in a two‐step mechanism of synthesis of α‐glucan bound to protein, are performed. Urea/sodium dodecyl sulfate/polyacrylamide gel electrophoretic analysis of the radioactive products synthesized by the reconstituted system sh...
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| Autores principales: | , , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00142956_v162_n3_p609_MORENO |
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| Sumario: | Reconstitution experiments with the DEAE‐cellulose‐treated enzymes, engaged in a two‐step mechanism of synthesis of α‐glucan bound to protein, are performed. Urea/sodium dodecyl sulfate/polyacrylamide gel electrophoretic analysis of the radioactive products synthesized by the reconstituted system shows highly glucosylated, labeled bands, whose apparent molecular masses change with the acrylamide concentration in the gels. The long carbohydrate chains synthesized during the second step arise from the sequential addition of glucosyl moieties to the glucoprotein formed during the first step. A deglucosylation experiment confirms that the product of the reconstituted system originates from the 38‐kDa glucosylated component of the reaction 1 product by the addition of β‐amylase‐sensitive glucosyl moieties. Our data suggest that specific phosphorylases and starch synthetases are found in potato tuber, which are capable of utilizing reaction 1 product as primer for the synthesis of protein‐bound glucan. Copyright © 1987, Wiley Blackwell. All rights reserved |
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