α‐Glucan synthesis on a protein primer: A reconstituted system for the formation of protein‐bound α‐glucan
Reconstitution experiments with the DEAE‐cellulose‐treated enzymes, engaged in a two‐step mechanism of synthesis of α‐glucan bound to protein, are performed. Urea/sodium dodecyl sulfate/polyacrylamide gel electrophoretic analysis of the radioactive products synthesized by the reconstituted system sh...
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todo:paper_00142956_v162_n3_p609_MORENO2023-10-03T14:11:50Z α‐Glucan synthesis on a protein primer: A reconstituted system for the formation of protein‐bound α‐glucan MORENO, S. CARDINI, C.E. TANDECARZ, J.S. 1,4 glucan 1,4-glucan beta amylase glucan phosphorylase starch synthase vegetable protein article biosynthesis catalysis fluorography metabolism polyacrylamide gel electrophoresis potato protein binding beta-Amylase Catalysis Electrophoresis, Polyacrylamide Gel Glucans Phosphorylases Photofluorography Plant Proteins Potatoes Protein Binding Starch Synthase Support, Non-U.S. Gov't Reconstitution experiments with the DEAE‐cellulose‐treated enzymes, engaged in a two‐step mechanism of synthesis of α‐glucan bound to protein, are performed. Urea/sodium dodecyl sulfate/polyacrylamide gel electrophoretic analysis of the radioactive products synthesized by the reconstituted system shows highly glucosylated, labeled bands, whose apparent molecular masses change with the acrylamide concentration in the gels. The long carbohydrate chains synthesized during the second step arise from the sequential addition of glucosyl moieties to the glucoprotein formed during the first step. A deglucosylation experiment confirms that the product of the reconstituted system originates from the 38‐kDa glucosylated component of the reaction 1 product by the addition of β‐amylase‐sensitive glucosyl moieties. Our data suggest that specific phosphorylases and starch synthetases are found in potato tuber, which are capable of utilizing reaction 1 product as primer for the synthesis of protein‐bound glucan. Copyright © 1987, Wiley Blackwell. All rights reserved Fil:MORENO, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:TANDECARZ, J.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142956_v162_n3_p609_MORENO |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
1,4 glucan 1,4-glucan beta amylase glucan phosphorylase starch synthase vegetable protein article biosynthesis catalysis fluorography metabolism polyacrylamide gel electrophoresis potato protein binding beta-Amylase Catalysis Electrophoresis, Polyacrylamide Gel Glucans Phosphorylases Photofluorography Plant Proteins Potatoes Protein Binding Starch Synthase Support, Non-U.S. Gov't |
| spellingShingle |
1,4 glucan 1,4-glucan beta amylase glucan phosphorylase starch synthase vegetable protein article biosynthesis catalysis fluorography metabolism polyacrylamide gel electrophoresis potato protein binding beta-Amylase Catalysis Electrophoresis, Polyacrylamide Gel Glucans Phosphorylases Photofluorography Plant Proteins Potatoes Protein Binding Starch Synthase Support, Non-U.S. Gov't MORENO, S. CARDINI, C.E. TANDECARZ, J.S. α‐Glucan synthesis on a protein primer: A reconstituted system for the formation of protein‐bound α‐glucan |
| topic_facet |
1,4 glucan 1,4-glucan beta amylase glucan phosphorylase starch synthase vegetable protein article biosynthesis catalysis fluorography metabolism polyacrylamide gel electrophoresis potato protein binding beta-Amylase Catalysis Electrophoresis, Polyacrylamide Gel Glucans Phosphorylases Photofluorography Plant Proteins Potatoes Protein Binding Starch Synthase Support, Non-U.S. Gov't |
| description |
Reconstitution experiments with the DEAE‐cellulose‐treated enzymes, engaged in a two‐step mechanism of synthesis of α‐glucan bound to protein, are performed. Urea/sodium dodecyl sulfate/polyacrylamide gel electrophoretic analysis of the radioactive products synthesized by the reconstituted system shows highly glucosylated, labeled bands, whose apparent molecular masses change with the acrylamide concentration in the gels. The long carbohydrate chains synthesized during the second step arise from the sequential addition of glucosyl moieties to the glucoprotein formed during the first step. A deglucosylation experiment confirms that the product of the reconstituted system originates from the 38‐kDa glucosylated component of the reaction 1 product by the addition of β‐amylase‐sensitive glucosyl moieties. Our data suggest that specific phosphorylases and starch synthetases are found in potato tuber, which are capable of utilizing reaction 1 product as primer for the synthesis of protein‐bound glucan. Copyright © 1987, Wiley Blackwell. All rights reserved |
| format |
JOUR |
| author |
MORENO, S. CARDINI, C.E. TANDECARZ, J.S. |
| author_facet |
MORENO, S. CARDINI, C.E. TANDECARZ, J.S. |
| author_sort |
MORENO, S. |
| title |
α‐Glucan synthesis on a protein primer: A reconstituted system for the formation of protein‐bound α‐glucan |
| title_short |
α‐Glucan synthesis on a protein primer: A reconstituted system for the formation of protein‐bound α‐glucan |
| title_full |
α‐Glucan synthesis on a protein primer: A reconstituted system for the formation of protein‐bound α‐glucan |
| title_fullStr |
α‐Glucan synthesis on a protein primer: A reconstituted system for the formation of protein‐bound α‐glucan |
| title_full_unstemmed |
α‐Glucan synthesis on a protein primer: A reconstituted system for the formation of protein‐bound α‐glucan |
| title_sort |
α‐glucan synthesis on a protein primer: a reconstituted system for the formation of protein‐bound α‐glucan |
| url |
http://hdl.handle.net/20.500.12110/paper_00142956_v162_n3_p609_MORENO |
| work_keys_str_mv |
AT morenos aglucansynthesisonaproteinprimerareconstitutedsystemfortheformationofproteinboundaglucan AT cardinice aglucansynthesisonaproteinprimerareconstitutedsystemfortheformationofproteinboundaglucan AT tandecarzjs aglucansynthesisonaproteinprimerareconstitutedsystemfortheformationofproteinboundaglucan |
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1782025817656655872 |