Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP
The synthesis of phosphatidyl‐dCMP in mouse thymocytes is inhibited by the antineoplastic agent 1‐β‐d‐arabinofuranosyl cytosine (AraCyt)50% inhibition (ID50) being reached at an AraCyt concentration of 0.18 mM. IN the same cells, ID50 for DNA synthesis is 0.03 mM. This inhibition is probably mediate...
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todo:paper_00142956_v130_n2_p293_IUJVIDIN2023-10-03T14:11:47Z Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP IUJVIDIN, S. FELEDI, C. MEDRANO, E.E. MORDOH, J. cytarabine deoxyribonuclease radioisotope cancer chemotherapy drug inhibition in vitro study mouse nonhuman phosphatidyldeoxycytidine phosphate therapy thymocyte thymus Animal Cytarabine Deoxycytidine Monophosphate Deoxycytosine Nucleotides In Vitro Mice Mice, Inbred BALB C Support, Non-U.S. Gov't Thymus Gland The synthesis of phosphatidyl‐dCMP in mouse thymocytes is inhibited by the antineoplastic agent 1‐β‐d‐arabinofuranosyl cytosine (AraCyt)50% inhibition (ID50) being reached at an AraCyt concentration of 0.18 mM. IN the same cells, ID50 for DNA synthesis is 0.03 mM. This inhibition is probably mediated by the phosphorylated derivative of AraCyt (aCTP) since the synthesis of phosphatidyl‐dCMP from dCTP using permeabilized thymocytes is inhibited by a aCTP (ID50= 0.11 mM). The incorporatioin of [3H]AraCyt inbto the organic phase could also be detected, suggesting that this drug may act as a substrate for the enzyme that catalyzes the transfer of dCTP into phosphatidic acid. Copyright © 1983, Wiley Blackwell. All rights reserved Fil:IUJVIDIN, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142956_v130_n2_p293_IUJVIDIN |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cytarabine deoxyribonuclease radioisotope cancer chemotherapy drug inhibition in vitro study mouse nonhuman phosphatidyldeoxycytidine phosphate therapy thymocyte thymus Animal Cytarabine Deoxycytidine Monophosphate Deoxycytosine Nucleotides In Vitro Mice Mice, Inbred BALB C Support, Non-U.S. Gov't Thymus Gland |
| spellingShingle |
cytarabine deoxyribonuclease radioisotope cancer chemotherapy drug inhibition in vitro study mouse nonhuman phosphatidyldeoxycytidine phosphate therapy thymocyte thymus Animal Cytarabine Deoxycytidine Monophosphate Deoxycytosine Nucleotides In Vitro Mice Mice, Inbred BALB C Support, Non-U.S. Gov't Thymus Gland IUJVIDIN, S. FELEDI, C. MEDRANO, E.E. MORDOH, J. Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP |
| topic_facet |
cytarabine deoxyribonuclease radioisotope cancer chemotherapy drug inhibition in vitro study mouse nonhuman phosphatidyldeoxycytidine phosphate therapy thymocyte thymus Animal Cytarabine Deoxycytidine Monophosphate Deoxycytosine Nucleotides In Vitro Mice Mice, Inbred BALB C Support, Non-U.S. Gov't Thymus Gland |
| description |
The synthesis of phosphatidyl‐dCMP in mouse thymocytes is inhibited by the antineoplastic agent 1‐β‐d‐arabinofuranosyl cytosine (AraCyt)50% inhibition (ID50) being reached at an AraCyt concentration of 0.18 mM. IN the same cells, ID50 for DNA synthesis is 0.03 mM. This inhibition is probably mediated by the phosphorylated derivative of AraCyt (aCTP) since the synthesis of phosphatidyl‐dCMP from dCTP using permeabilized thymocytes is inhibited by a aCTP (ID50= 0.11 mM). The incorporatioin of [3H]AraCyt inbto the organic phase could also be detected, suggesting that this drug may act as a substrate for the enzyme that catalyzes the transfer of dCTP into phosphatidic acid. Copyright © 1983, Wiley Blackwell. All rights reserved |
| format |
JOUR |
| author |
IUJVIDIN, S. FELEDI, C. MEDRANO, E.E. MORDOH, J. |
| author_facet |
IUJVIDIN, S. FELEDI, C. MEDRANO, E.E. MORDOH, J. |
| author_sort |
IUJVIDIN, S. |
| title |
Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP |
| title_short |
Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP |
| title_full |
Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP |
| title_fullStr |
Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP |
| title_full_unstemmed |
Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP |
| title_sort |
inhibitory effect of 1‐β‐d‐arabinofuranosylcytosine on the synthesis of phosphatidyl‐dcmp |
| url |
http://hdl.handle.net/20.500.12110/paper_00142956_v130_n2_p293_IUJVIDIN |
| work_keys_str_mv |
AT iujvidins inhibitoryeffectof1bdarabinofuranosylcytosineonthesynthesisofphosphatidyldcmp AT feledic inhibitoryeffectof1bdarabinofuranosylcytosineonthesynthesisofphosphatidyldcmp AT medranoee inhibitoryeffectof1bdarabinofuranosylcytosineonthesynthesisofphosphatidyldcmp AT mordohj inhibitoryeffectof1bdarabinofuranosylcytosineonthesynthesisofphosphatidyldcmp |
| _version_ |
1807320112260186112 |