Biosynthesis of Polysaccharides in Acetobacter xylinum Sequential Synthesis of a Heptasaccharide Diphosphate Prenol

The sequential synthesis in vitro of a heptasaccharide diphosphate prenol, containing glucose, mannose, glucuronic acid and rhamnose in the ratio 4:1:1:1 is described. The enzyme preparation consisted of EDTA‐treated Acetobacter xylinum cells and UDP‐glucose, GDO‐mannose, UDP‐glucuronic acid and TDP...

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Autores principales: COUSO, R.O., IELPI, L., GARCIA, R.C., DANKERT, M.A.
Formato: JOUR
Materias:
polysaccharide
acetobacter xylinum
animal experiment
in vitro study
Acetobacter
Chemistry
Hydrolysis
Polyisoprenyl Phosphate Oligosaccharides
Polyisoprenyl Phosphate Sugars
Polysaccharides, Bacterial
Rhamnose
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00142956_v123_n3_p617_COUSO
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00142956_v123_n3_p617_COUSO
record_format dspace
spelling todo:paper_00142956_v123_n3_p617_COUSO2023-10-03T14:11:47Z Biosynthesis of Polysaccharides in Acetobacter xylinum Sequential Synthesis of a Heptasaccharide Diphosphate Prenol COUSO, R.O. IELPI, L. GARCIA, R.C. DANKERT, M.A. polysaccharide acetobacter xylinum animal experiment in vitro study Acetobacter Chemistry Hydrolysis Polyisoprenyl Phosphate Oligosaccharides Polyisoprenyl Phosphate Sugars Polysaccharides, Bacterial Rhamnose The sequential synthesis in vitro of a heptasaccharide diphosphate prenol, containing glucose, mannose, glucuronic acid and rhamnose in the ratio 4:1:1:1 is described. The enzyme preparation consisted of EDTA‐treated Acetobacter xylinum cells and UDP‐glucose, GDO‐mannose, UDP‐glucuronic acid and TDP‐rhamnose were employed as sugar donors. The compounds soluble in chloroform/methanol/water (1:2:0.3) formed from incubations carried out under different conditions in the presence of a variety of combinations of the donors labeled with 14C, 3H or 32P were analysed by DEAE‐cellulose column chromatography, gel filtraton, partial acid hydrolysis, acetolysis, periodate oxidation, etc. The following structure is proposed for the most complex compound characterized: rhamnosyL(1 → 6)‐β‐glucosyl‐(1 → 6)‐α‐glucosyl‐(1 → 4)‐β‐glucuronyl‐(1 → 6)‐β‐mannosyl‐(1 → 3)‐β‐glucosyl‐(1 → 4)‐α‐glucosyl diphosphate prenol. The smaller oligosaccharide diphosphate prenols formed as intermediate steps are also characterized in this or in previous work [Garcia, R. C., Recondo, E. and Dankert, M. A. (1974) Eur. J. Biochem. 43, 93–105; Couso, R. O., Ielpi, L., and Damkert, M. A. (1980) Arch. Biochem. Biophys. 204, 434–443]. The role of these compounds in the biosynthesis of a complex exopolysaccharide that this microorganism forms in addition to cellulose is discussed. Copyright © 1982, Wiley Blackwell. All rights reserved JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142956_v123_n3_p617_COUSO
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic polysaccharide
acetobacter xylinum
animal experiment
in vitro study
Acetobacter
Chemistry
Hydrolysis
Polyisoprenyl Phosphate Oligosaccharides
Polyisoprenyl Phosphate Sugars
Polysaccharides, Bacterial
Rhamnose
spellingShingle polysaccharide
acetobacter xylinum
animal experiment
in vitro study
Acetobacter
Chemistry
Hydrolysis
Polyisoprenyl Phosphate Oligosaccharides
Polyisoprenyl Phosphate Sugars
Polysaccharides, Bacterial
Rhamnose
COUSO, R.O.
IELPI, L.
GARCIA, R.C.
DANKERT, M.A.
Biosynthesis of Polysaccharides in Acetobacter xylinum Sequential Synthesis of a Heptasaccharide Diphosphate Prenol
topic_facet polysaccharide
acetobacter xylinum
animal experiment
in vitro study
Acetobacter
Chemistry
Hydrolysis
Polyisoprenyl Phosphate Oligosaccharides
Polyisoprenyl Phosphate Sugars
Polysaccharides, Bacterial
Rhamnose
description The sequential synthesis in vitro of a heptasaccharide diphosphate prenol, containing glucose, mannose, glucuronic acid and rhamnose in the ratio 4:1:1:1 is described. The enzyme preparation consisted of EDTA‐treated Acetobacter xylinum cells and UDP‐glucose, GDO‐mannose, UDP‐glucuronic acid and TDP‐rhamnose were employed as sugar donors. The compounds soluble in chloroform/methanol/water (1:2:0.3) formed from incubations carried out under different conditions in the presence of a variety of combinations of the donors labeled with 14C, 3H or 32P were analysed by DEAE‐cellulose column chromatography, gel filtraton, partial acid hydrolysis, acetolysis, periodate oxidation, etc. The following structure is proposed for the most complex compound characterized: rhamnosyL(1 → 6)‐β‐glucosyl‐(1 → 6)‐α‐glucosyl‐(1 → 4)‐β‐glucuronyl‐(1 → 6)‐β‐mannosyl‐(1 → 3)‐β‐glucosyl‐(1 → 4)‐α‐glucosyl diphosphate prenol. The smaller oligosaccharide diphosphate prenols formed as intermediate steps are also characterized in this or in previous work [Garcia, R. C., Recondo, E. and Dankert, M. A. (1974) Eur. J. Biochem. 43, 93–105; Couso, R. O., Ielpi, L., and Damkert, M. A. (1980) Arch. Biochem. Biophys. 204, 434–443]. The role of these compounds in the biosynthesis of a complex exopolysaccharide that this microorganism forms in addition to cellulose is discussed. Copyright © 1982, Wiley Blackwell. All rights reserved
format JOUR
author COUSO, R.O.
IELPI, L.
GARCIA, R.C.
DANKERT, M.A.
author_facet COUSO, R.O.
IELPI, L.
GARCIA, R.C.
DANKERT, M.A.
author_sort COUSO, R.O.
title Biosynthesis of Polysaccharides in Acetobacter xylinum Sequential Synthesis of a Heptasaccharide Diphosphate Prenol
title_short Biosynthesis of Polysaccharides in Acetobacter xylinum Sequential Synthesis of a Heptasaccharide Diphosphate Prenol
title_full Biosynthesis of Polysaccharides in Acetobacter xylinum Sequential Synthesis of a Heptasaccharide Diphosphate Prenol
title_fullStr Biosynthesis of Polysaccharides in Acetobacter xylinum Sequential Synthesis of a Heptasaccharide Diphosphate Prenol
title_full_unstemmed Biosynthesis of Polysaccharides in Acetobacter xylinum Sequential Synthesis of a Heptasaccharide Diphosphate Prenol
title_sort biosynthesis of polysaccharides in acetobacter xylinum sequential synthesis of a heptasaccharide diphosphate prenol
url http://hdl.handle.net/20.500.12110/paper_00142956_v123_n3_p617_COUSO
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AT garciarc biosynthesisofpolysaccharidesinacetobacterxylinumsequentialsynthesisofaheptasaccharidediphosphateprenol
AT dankertma biosynthesisofpolysaccharidesinacetobacterxylinumsequentialsynthesisofaheptasaccharidediphosphateprenol
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