Synthesis of Phosphatidyl‐dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine
When phytohemagglutinin‐stimulated lymphocytes are labelled with [3H]deoxycytidine (dCyd), a compound soluble in organic solvents is formed. Several analytical procedures such as thin‐layer chromatography and alkaline hydrolysis of the product obtained after labelling of stimulated lymphocytes with...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00142956_v116_n2_p249_MEDRANO |
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todo:paper_00142956_v116_n2_p249_MEDRANO2023-10-03T14:11:46Z Synthesis of Phosphatidyl‐dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine MEDRANO, E. IUJVIDIN, S. MORDOH, J. chlorpromazine phosphatidylinositol radioisotope biosynthesis blood and hemopoietic system deoxycytidine h 3 human cell in vitro study lymphatic system lymphocyte normal human phosphatidyldeoxycytidine phosphate Chlorpromazine Deoxycytidine Deoxycytidine Monophosphate Deoxycytosine Nucleotides Dose-Response Relationship, Drug Human In Vitro Kinetics Lymphocyte Activation Lymphocytes Phosphatidic Acids Phytohemagglutinins Support, Non-U.S. Gov't When phytohemagglutinin‐stimulated lymphocytes are labelled with [3H]deoxycytidine (dCyd), a compound soluble in organic solvents is formed. Several analytical procedures such as thin‐layer chromatography and alkaline hydrolysis of the product obtained after labelling of stimulated lymphocytes with [14C]glycerol and [3H]dCyd, suggest that the product synthesized is phosphatidyl‐dCMP. This compound is synthesized in much smaller amounts than when permeabilized lymphocytes and [3H]dCTP as a precursor are used. The synthesis of phosphatidyl‐dCMP is highly enhanced by chlorpromazine, phosphatidic acid and arachidonic acid, whereas addition of inositol diminishes the amount of detected phosphatidyl‐dCMP. It is suggested that this compound would be a precursor of phosphatidylinositol. Copyright © 1981, Wiley Blackwell. All rights reserved JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142956_v116_n2_p249_MEDRANO |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
chlorpromazine phosphatidylinositol radioisotope biosynthesis blood and hemopoietic system deoxycytidine h 3 human cell in vitro study lymphatic system lymphocyte normal human phosphatidyldeoxycytidine phosphate Chlorpromazine Deoxycytidine Deoxycytidine Monophosphate Deoxycytosine Nucleotides Dose-Response Relationship, Drug Human In Vitro Kinetics Lymphocyte Activation Lymphocytes Phosphatidic Acids Phytohemagglutinins Support, Non-U.S. Gov't |
| spellingShingle |
chlorpromazine phosphatidylinositol radioisotope biosynthesis blood and hemopoietic system deoxycytidine h 3 human cell in vitro study lymphatic system lymphocyte normal human phosphatidyldeoxycytidine phosphate Chlorpromazine Deoxycytidine Deoxycytidine Monophosphate Deoxycytosine Nucleotides Dose-Response Relationship, Drug Human In Vitro Kinetics Lymphocyte Activation Lymphocytes Phosphatidic Acids Phytohemagglutinins Support, Non-U.S. Gov't MEDRANO, E. IUJVIDIN, S. MORDOH, J. Synthesis of Phosphatidyl‐dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine |
| topic_facet |
chlorpromazine phosphatidylinositol radioisotope biosynthesis blood and hemopoietic system deoxycytidine h 3 human cell in vitro study lymphatic system lymphocyte normal human phosphatidyldeoxycytidine phosphate Chlorpromazine Deoxycytidine Deoxycytidine Monophosphate Deoxycytosine Nucleotides Dose-Response Relationship, Drug Human In Vitro Kinetics Lymphocyte Activation Lymphocytes Phosphatidic Acids Phytohemagglutinins Support, Non-U.S. Gov't |
| description |
When phytohemagglutinin‐stimulated lymphocytes are labelled with [3H]deoxycytidine (dCyd), a compound soluble in organic solvents is formed. Several analytical procedures such as thin‐layer chromatography and alkaline hydrolysis of the product obtained after labelling of stimulated lymphocytes with [14C]glycerol and [3H]dCyd, suggest that the product synthesized is phosphatidyl‐dCMP. This compound is synthesized in much smaller amounts than when permeabilized lymphocytes and [3H]dCTP as a precursor are used. The synthesis of phosphatidyl‐dCMP is highly enhanced by chlorpromazine, phosphatidic acid and arachidonic acid, whereas addition of inositol diminishes the amount of detected phosphatidyl‐dCMP. It is suggested that this compound would be a precursor of phosphatidylinositol. Copyright © 1981, Wiley Blackwell. All rights reserved |
| format |
JOUR |
| author |
MEDRANO, E. IUJVIDIN, S. MORDOH, J. |
| author_facet |
MEDRANO, E. IUJVIDIN, S. MORDOH, J. |
| author_sort |
MEDRANO, E. |
| title |
Synthesis of Phosphatidyl‐dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine |
| title_short |
Synthesis of Phosphatidyl‐dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine |
| title_full |
Synthesis of Phosphatidyl‐dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine |
| title_fullStr |
Synthesis of Phosphatidyl‐dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine |
| title_full_unstemmed |
Synthesis of Phosphatidyl‐dCMP in Human Lymphocytes and Its Enhancement by Chlorpromazine |
| title_sort |
synthesis of phosphatidyl‐dcmp in human lymphocytes and its enhancement by chlorpromazine |
| url |
http://hdl.handle.net/20.500.12110/paper_00142956_v116_n2_p249_MEDRANO |
| work_keys_str_mv |
AT medranoe synthesisofphosphatidyldcmpinhumanlymphocytesanditsenhancementbychlorpromazine AT iujvidins synthesisofphosphatidyldcmpinhumanlymphocytesanditsenhancementbychlorpromazine AT mordohj synthesisofphosphatidyldcmpinhumanlymphocytesanditsenhancementbychlorpromazine |
| _version_ |
1807323279463022592 |