Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean

Use of molecular markers such as Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP) and Simple Sequence Repeats (SSR) as descriptors to characterize and differentiate a set of 100 soybean varieties of commercial use in Argentina was taken as a leading case study f...

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Autores principales: Giancola, S., Marcucci Poltri, S., Lacaze, P., Hopp, H.E.
Formato: JOUR
Materias:
AFLP
Glycine max (L.) Merr.
RAPD
SSR
UPGMA
Embryophyta
Glycine max
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00142336_v127_n1_p95_Giancola
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00142336_v127_n1_p95_Giancola
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spelling todo:paper_00142336_v127_n1_p95_Giancola2023-10-03T14:11:43Z Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean Giancola, S. Marcucci Poltri, S. Lacaze, P. Hopp, H.E. AFLP Glycine max (L.) Merr. RAPD SSR UPGMA Embryophyta Glycine max Use of molecular markers such as Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP) and Simple Sequence Repeats (SSR) as descriptors to characterize and differentiate a set of 100 soybean varieties of commercial use in Argentina was taken as a leading case study for plant variety protection (PVP) purposes. Sixteen morphological traits were recorded to compare pedigree relationships among varieties with information derived from conventional descriptors and molecular markers. Analysis of 109 polymorphic loci confirmed the rather low genetic variability of commercial soybean germplasm. Still, genetic fingerprinting of the 100 varieties could be established. Calculated similarity indexes were dependent on the technique, ranging from 0.262 (SSR), 0.407 (RAPD), 0.400 (AFLP) and 0.574 (morphological traits). Dendrograms generated from morphological data matrix showed low value correlation with kinship coefficient matrix (r = 0.216). Still, they were suitable to identify and differentiate each of the 100 varieties analyzed; which was not possible with RAPD or AFLP markers using comparable numbers of polymorphic loci. SSR data showed the best fit to pedigree information (r = 0.353), while maintaining an association to morphologically based separation. Results suggest that the four techniques describe genetic variability in different and specific ways. A combination of SSR and morphological descriptors show the best compromise of regarding genetic relationships and the needs of clear classification for PVP and may help to establish minimum genetic distances for distinctness within PVP Office definition. Fil:Giancola, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marcucci Poltri, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142336_v127_n1_p95_Giancola
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic AFLP
Glycine max (L.) Merr.
RAPD
SSR
UPGMA
Embryophyta
Glycine max
spellingShingle AFLP
Glycine max (L.) Merr.
RAPD
SSR
UPGMA
Embryophyta
Glycine max
Giancola, S.
Marcucci Poltri, S.
Lacaze, P.
Hopp, H.E.
Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean
topic_facet AFLP
Glycine max (L.) Merr.
RAPD
SSR
UPGMA
Embryophyta
Glycine max
description Use of molecular markers such as Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP) and Simple Sequence Repeats (SSR) as descriptors to characterize and differentiate a set of 100 soybean varieties of commercial use in Argentina was taken as a leading case study for plant variety protection (PVP) purposes. Sixteen morphological traits were recorded to compare pedigree relationships among varieties with information derived from conventional descriptors and molecular markers. Analysis of 109 polymorphic loci confirmed the rather low genetic variability of commercial soybean germplasm. Still, genetic fingerprinting of the 100 varieties could be established. Calculated similarity indexes were dependent on the technique, ranging from 0.262 (SSR), 0.407 (RAPD), 0.400 (AFLP) and 0.574 (morphological traits). Dendrograms generated from morphological data matrix showed low value correlation with kinship coefficient matrix (r = 0.216). Still, they were suitable to identify and differentiate each of the 100 varieties analyzed; which was not possible with RAPD or AFLP markers using comparable numbers of polymorphic loci. SSR data showed the best fit to pedigree information (r = 0.353), while maintaining an association to morphologically based separation. Results suggest that the four techniques describe genetic variability in different and specific ways. A combination of SSR and morphological descriptors show the best compromise of regarding genetic relationships and the needs of clear classification for PVP and may help to establish minimum genetic distances for distinctness within PVP Office definition.
format JOUR
author Giancola, S.
Marcucci Poltri, S.
Lacaze, P.
Hopp, H.E.
author_facet Giancola, S.
Marcucci Poltri, S.
Lacaze, P.
Hopp, H.E.
author_sort Giancola, S.
title Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean
title_short Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean
title_full Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean
title_fullStr Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean
title_full_unstemmed Feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean
title_sort feasibility of integration of molecular markers and morphological descriptors in a real case study of a plant variety protection system for soybean
url http://hdl.handle.net/20.500.12110/paper_00142336_v127_n1_p95_Giancola
work_keys_str_mv AT giancolas feasibilityofintegrationofmolecularmarkersandmorphologicaldescriptorsinarealcasestudyofaplantvarietyprotectionsystemforsoybean
AT marcuccipoltris feasibilityofintegrationofmolecularmarkersandmorphologicaldescriptorsinarealcasestudyofaplantvarietyprotectionsystemforsoybean
AT lacazep feasibilityofintegrationofmolecularmarkersandmorphologicaldescriptorsinarealcasestudyofaplantvarietyprotectionsystemforsoybean
AT hopphe feasibilityofintegrationofmolecularmarkersandmorphologicaldescriptorsinarealcasestudyofaplantvarietyprotectionsystemforsoybean
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