Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells
Alternative pre-mRNA splicing plays key roles in determining tissue- and species-specific cell differentiation as well as in the onset of hereditary disease and cancer, being controlled by multiple post- and co-transcriptional regulatory mechanisms. We report here that airborne particulate matter, r...
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todo:paper_00139351_v140_n_p185_Buggiano2023-10-03T14:11:37Z Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells Buggiano, V. Petrillo, E. Alló, M. Lafaille, C. Redal, M.A. Alghamdi, M.A. Khoder, M.I. Shamy, M. Muñoz, M.J. Kornblihtt, A.R. Air pollution Alternative splicing Bone morphogenetic protein Colon cancer cells Particulate matter bone morphogenetic protein messenger RNA bone morphogenetic protein 4 messenger RNA particulate matter primer DNA RNA precursor atmospheric pollution cancer cytology industrial waste particulate matter protein RNA 5' untranslated region air pollution alternative RNA splicing Article cancer cell culture cancer cell line cell proliferation colon cancer colon carcinogenesis controlled study human human cell particulate matter priority journal transcription elongation alternative RNA splicing colon tumor drug effects genetics HEK293 cell line nucleotide sequence particulate matter pathology pharmacology reverse transcription polymerase chain reaction tumor cell line Alternative Splicing Base Sequence Bone Morphogenetic Protein 4 Cell Line, Tumor Colonic Neoplasms DNA Primers HEK293 Cells Humans Particulate Matter Reverse Transcriptase Polymerase Chain Reaction RNA Precursors RNA, Messenger Alternative pre-mRNA splicing plays key roles in determining tissue- and species-specific cell differentiation as well as in the onset of hereditary disease and cancer, being controlled by multiple post- and co-transcriptional regulatory mechanisms. We report here that airborne particulate matter, resulting from industrial pollution, inhibits expression and specifically affects alternative splicing at the 5' untranslated region of the mRNA encoding the bone morphogenetic protein BMP4 in human colon cells in culture. These effects are consistent with a previously reported role for BMP4 in preventing colon cancer development, suggesting that ingestion of particulate matter could contribute to the onset of colon cell proliferation. We also show that the underlying mechanism might involve changes in transcriptional elongation. This is the first study to demonstrate that particulate matter causes non-pleiotropic changes in alternative splicing. © 2015 Elsevier Inc. Fil:Petrillo, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alló, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lafaille, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muñoz, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00139351_v140_n_p185_Buggiano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Air pollution Alternative splicing Bone morphogenetic protein Colon cancer cells Particulate matter bone morphogenetic protein messenger RNA bone morphogenetic protein 4 messenger RNA particulate matter primer DNA RNA precursor atmospheric pollution cancer cytology industrial waste particulate matter protein RNA 5' untranslated region air pollution alternative RNA splicing Article cancer cell culture cancer cell line cell proliferation colon cancer colon carcinogenesis controlled study human human cell particulate matter priority journal transcription elongation alternative RNA splicing colon tumor drug effects genetics HEK293 cell line nucleotide sequence particulate matter pathology pharmacology reverse transcription polymerase chain reaction tumor cell line Alternative Splicing Base Sequence Bone Morphogenetic Protein 4 Cell Line, Tumor Colonic Neoplasms DNA Primers HEK293 Cells Humans Particulate Matter Reverse Transcriptase Polymerase Chain Reaction RNA Precursors RNA, Messenger |
| spellingShingle |
Air pollution Alternative splicing Bone morphogenetic protein Colon cancer cells Particulate matter bone morphogenetic protein messenger RNA bone morphogenetic protein 4 messenger RNA particulate matter primer DNA RNA precursor atmospheric pollution cancer cytology industrial waste particulate matter protein RNA 5' untranslated region air pollution alternative RNA splicing Article cancer cell culture cancer cell line cell proliferation colon cancer colon carcinogenesis controlled study human human cell particulate matter priority journal transcription elongation alternative RNA splicing colon tumor drug effects genetics HEK293 cell line nucleotide sequence particulate matter pathology pharmacology reverse transcription polymerase chain reaction tumor cell line Alternative Splicing Base Sequence Bone Morphogenetic Protein 4 Cell Line, Tumor Colonic Neoplasms DNA Primers HEK293 Cells Humans Particulate Matter Reverse Transcriptase Polymerase Chain Reaction RNA Precursors RNA, Messenger Buggiano, V. Petrillo, E. Alló, M. Lafaille, C. Redal, M.A. Alghamdi, M.A. Khoder, M.I. Shamy, M. Muñoz, M.J. Kornblihtt, A.R. Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells |
| topic_facet |
Air pollution Alternative splicing Bone morphogenetic protein Colon cancer cells Particulate matter bone morphogenetic protein messenger RNA bone morphogenetic protein 4 messenger RNA particulate matter primer DNA RNA precursor atmospheric pollution cancer cytology industrial waste particulate matter protein RNA 5' untranslated region air pollution alternative RNA splicing Article cancer cell culture cancer cell line cell proliferation colon cancer colon carcinogenesis controlled study human human cell particulate matter priority journal transcription elongation alternative RNA splicing colon tumor drug effects genetics HEK293 cell line nucleotide sequence particulate matter pathology pharmacology reverse transcription polymerase chain reaction tumor cell line Alternative Splicing Base Sequence Bone Morphogenetic Protein 4 Cell Line, Tumor Colonic Neoplasms DNA Primers HEK293 Cells Humans Particulate Matter Reverse Transcriptase Polymerase Chain Reaction RNA Precursors RNA, Messenger |
| description |
Alternative pre-mRNA splicing plays key roles in determining tissue- and species-specific cell differentiation as well as in the onset of hereditary disease and cancer, being controlled by multiple post- and co-transcriptional regulatory mechanisms. We report here that airborne particulate matter, resulting from industrial pollution, inhibits expression and specifically affects alternative splicing at the 5' untranslated region of the mRNA encoding the bone morphogenetic protein BMP4 in human colon cells in culture. These effects are consistent with a previously reported role for BMP4 in preventing colon cancer development, suggesting that ingestion of particulate matter could contribute to the onset of colon cell proliferation. We also show that the underlying mechanism might involve changes in transcriptional elongation. This is the first study to demonstrate that particulate matter causes non-pleiotropic changes in alternative splicing. © 2015 Elsevier Inc. |
| format |
JOUR |
| author |
Buggiano, V. Petrillo, E. Alló, M. Lafaille, C. Redal, M.A. Alghamdi, M.A. Khoder, M.I. Shamy, M. Muñoz, M.J. Kornblihtt, A.R. |
| author_facet |
Buggiano, V. Petrillo, E. Alló, M. Lafaille, C. Redal, M.A. Alghamdi, M.A. Khoder, M.I. Shamy, M. Muñoz, M.J. Kornblihtt, A.R. |
| author_sort |
Buggiano, V. |
| title |
Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells |
| title_short |
Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells |
| title_full |
Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells |
| title_fullStr |
Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells |
| title_full_unstemmed |
Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells |
| title_sort |
effects of airborne particulate matter on alternative pre-mrna splicing in colon cancer cells |
| url |
http://hdl.handle.net/20.500.12110/paper_00139351_v140_n_p185_Buggiano |
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