Origin and distribution of Ceratitis capitata mitochondrial DNA haplotypes in Argentina

The Mediterranean fruit fly, Ceratitis capitata (Wiedemann), considered as one of the most important agricultural pests, is broadly distributed around the world. From its presumed origin in Africa, the fly spread to several areas in Europe and, over the past 100 yr, to Australia and the Americas. We...

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Autores principales: Lanzavecchia, S.B., Cladera, J.L., Faccio, P., Marty, N.P., Vilardi, J.C., Zandomeni, R.O.
Formato: JOUR
Materias:
Ceratitis capitata
DNA molecular marker
Introduction sources
Mitochondrial haplotypes
Population dynamics
fly
genetic marker
mitochondrial DNA
polymerase chain reaction
polymorphism
population dynamics
Argentina
South America
Hexapoda
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00138746_v101_n3_p627_Lanzavecchia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00138746_v101_n3_p627_Lanzavecchia
record_format dspace
spelling todo:paper_00138746_v101_n3_p627_Lanzavecchia2023-10-03T14:11:31Z Origin and distribution of Ceratitis capitata mitochondrial DNA haplotypes in Argentina Lanzavecchia, S.B. Cladera, J.L. Faccio, P. Marty, N.P. Vilardi, J.C. Zandomeni, R.O. Ceratitis capitata DNA molecular marker Introduction sources Mitochondrial haplotypes Population dynamics fly genetic marker mitochondrial DNA polymerase chain reaction polymorphism population dynamics Argentina South America Ceratitis capitata Hexapoda The Mediterranean fruit fly, Ceratitis capitata (Wiedemann), considered as one of the most important agricultural pests, is broadly distributed around the world. From its presumed origin in Africa, the fly spread to several areas in Europe and, over the past 100 yr, to Australia and the Americas. We performed an extensive sampling of Mediterranean fruit fly populations in Argentina, covering most fruit-producing areas. The collection was studied using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) and sequencing of ND4 and ND5 genes in the mitochondrial genome. We followed the standard system of nomenclature devised previously for designating mitochondrial haplotypes. Our results revealed 1) a high frequency of the already reported AAC and BBB haplotypes; 2) the presence of the AAB haplotype at very low frequency not previously detected in Argentina; 3) a novel point mutation defining two variants of the AAB haplotype (AABA and AABB), and 4) the presence of the AAA haplotype, only in insects of the strain used in the sterile insect technique. A PCR-RFLP assay was developed to differentiate the AABA and AABB haplotypes. The sequence analysis suggests that the AAC haplotype is most likely derived from the AABA haplotype by one nucleotide change. Inferences about the origin of Mediterranean fruit fly populations in this country are discussed. © 2008 Entomological Society of America. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00138746_v101_n3_p627_Lanzavecchia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Ceratitis capitata
DNA molecular marker
Introduction sources
Mitochondrial haplotypes
Population dynamics
fly
genetic marker
mitochondrial DNA
polymerase chain reaction
polymorphism
population dynamics
Argentina
South America
Ceratitis capitata
Hexapoda
spellingShingle Ceratitis capitata
DNA molecular marker
Introduction sources
Mitochondrial haplotypes
Population dynamics
fly
genetic marker
mitochondrial DNA
polymerase chain reaction
polymorphism
population dynamics
Argentina
South America
Ceratitis capitata
Hexapoda
Lanzavecchia, S.B.
Cladera, J.L.
Faccio, P.
Marty, N.P.
Vilardi, J.C.
Zandomeni, R.O.
Origin and distribution of Ceratitis capitata mitochondrial DNA haplotypes in Argentina
topic_facet Ceratitis capitata
DNA molecular marker
Introduction sources
Mitochondrial haplotypes
Population dynamics
fly
genetic marker
mitochondrial DNA
polymerase chain reaction
polymorphism
population dynamics
Argentina
South America
Ceratitis capitata
Hexapoda
description The Mediterranean fruit fly, Ceratitis capitata (Wiedemann), considered as one of the most important agricultural pests, is broadly distributed around the world. From its presumed origin in Africa, the fly spread to several areas in Europe and, over the past 100 yr, to Australia and the Americas. We performed an extensive sampling of Mediterranean fruit fly populations in Argentina, covering most fruit-producing areas. The collection was studied using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) and sequencing of ND4 and ND5 genes in the mitochondrial genome. We followed the standard system of nomenclature devised previously for designating mitochondrial haplotypes. Our results revealed 1) a high frequency of the already reported AAC and BBB haplotypes; 2) the presence of the AAB haplotype at very low frequency not previously detected in Argentina; 3) a novel point mutation defining two variants of the AAB haplotype (AABA and AABB), and 4) the presence of the AAA haplotype, only in insects of the strain used in the sterile insect technique. A PCR-RFLP assay was developed to differentiate the AABA and AABB haplotypes. The sequence analysis suggests that the AAC haplotype is most likely derived from the AABA haplotype by one nucleotide change. Inferences about the origin of Mediterranean fruit fly populations in this country are discussed. © 2008 Entomological Society of America.
format JOUR
author Lanzavecchia, S.B.
Cladera, J.L.
Faccio, P.
Marty, N.P.
Vilardi, J.C.
Zandomeni, R.O.
author_facet Lanzavecchia, S.B.
Cladera, J.L.
Faccio, P.
Marty, N.P.
Vilardi, J.C.
Zandomeni, R.O.
author_sort Lanzavecchia, S.B.
title Origin and distribution of Ceratitis capitata mitochondrial DNA haplotypes in Argentina
title_short Origin and distribution of Ceratitis capitata mitochondrial DNA haplotypes in Argentina
title_full Origin and distribution of Ceratitis capitata mitochondrial DNA haplotypes in Argentina
title_fullStr Origin and distribution of Ceratitis capitata mitochondrial DNA haplotypes in Argentina
title_full_unstemmed Origin and distribution of Ceratitis capitata mitochondrial DNA haplotypes in Argentina
title_sort origin and distribution of ceratitis capitata mitochondrial dna haplotypes in argentina
url http://hdl.handle.net/20.500.12110/paper_00138746_v101_n3_p627_Lanzavecchia
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AT martynp originanddistributionofceratitiscapitatamitochondrialdnahaplotypesinargentina
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