Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata

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DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants wer...

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Autores principales: Lanzavecchia, S., Remis, M., Cladera, J., Zandomeni, R.
Formato: JOUR
Materias:
Diptera
EPIC-PCR
Exon priming intron crossing-polymerase chain reaction
Gene flow
Medfly
Migration patterns
Molecular markers
Population genetics
Tephritidae
allele
biomarker
fly
gene flow
genetic analysis
heterozygosity
migration
polymerase chain reaction
polymorphism
Ceratitis capitata
Hexapoda
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00138703_v136_n1_p53_Lanzavecchia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00138703_v136_n1_p53_Lanzavecchia
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spelling todo:paper_00138703_v136_n1_p53_Lanzavecchia2023-10-03T14:11:26Z Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata Lanzavecchia, S. Remis, M. Cladera, J. Zandomeni, R. Diptera EPIC-PCR Exon priming intron crossing-polymerase chain reaction Gene flow Medfly Migration patterns Molecular markers Population genetics Tephritidae allele biomarker fly gene flow genetic analysis heterozygosity migration polymerase chain reaction polymorphism Ceratitis capitata Diptera Hexapoda Tephritidae DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants were detected among the 307 samples analyzed. To better differentiate the size variants, further digestion of PCR products with the EcoRI restriction enzyme was carried out. Complete nucleotide sequences of the three-allele variants were obtained and single changes, insertions, deletions, and EcoRI recognition sites were located. Population allele frequencies were analyzed and a global mean heterozygosity (He) of 0.33 was obtained. In most populations, observed allelic frequencies conformed to Hardy-Weinberg expectations. Significant differences between provinces and sampling sites within these provinces, and among some populations were found. The average number of insects exchanged among populations (Nm) was estimated and high values were observed between Argentina and populations from two African countries (Morocco and Kenya), Australia, and Hawaii (Kauai). Pest introduction sources and dispersion patterns in Argentina are discussed based on these results as well as on available bibliographical data. © 2010 The Authors. Journal compilation © 2010 The Netherlands Entomological Society. Fil:Lanzavecchia, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Remis, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cladera, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00138703_v136_n1_p53_Lanzavecchia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Diptera
EPIC-PCR
Exon priming intron crossing-polymerase chain reaction
Gene flow
Medfly
Migration patterns
Molecular markers
Population genetics
Tephritidae
allele
biomarker
fly
gene flow
genetic analysis
heterozygosity
migration
polymerase chain reaction
polymorphism
Ceratitis capitata
Diptera
Hexapoda
Tephritidae
spellingShingle Diptera
EPIC-PCR
Exon priming intron crossing-polymerase chain reaction
Gene flow
Medfly
Migration patterns
Molecular markers
Population genetics
Tephritidae
allele
biomarker
fly
gene flow
genetic analysis
heterozygosity
migration
polymerase chain reaction
polymorphism
Ceratitis capitata
Diptera
Hexapoda
Tephritidae
Lanzavecchia, S.
Remis, M.
Cladera, J.
Zandomeni, R.
Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata
topic_facet Diptera
EPIC-PCR
Exon priming intron crossing-polymerase chain reaction
Gene flow
Medfly
Migration patterns
Molecular markers
Population genetics
Tephritidae
allele
biomarker
fly
gene flow
genetic analysis
heterozygosity
migration
polymerase chain reaction
polymorphism
Ceratitis capitata
Diptera
Hexapoda
Tephritidae
description DNA size polymorphisms were utilized in a study of 24 natural populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae) from Argentina. The first intron of alcohol dehydrogenase 1 gene (Adh1) was amplified using exon priming intron crossing-polymerase chain reaction. Three size variants were detected among the 307 samples analyzed. To better differentiate the size variants, further digestion of PCR products with the EcoRI restriction enzyme was carried out. Complete nucleotide sequences of the three-allele variants were obtained and single changes, insertions, deletions, and EcoRI recognition sites were located. Population allele frequencies were analyzed and a global mean heterozygosity (He) of 0.33 was obtained. In most populations, observed allelic frequencies conformed to Hardy-Weinberg expectations. Significant differences between provinces and sampling sites within these provinces, and among some populations were found. The average number of insects exchanged among populations (Nm) was estimated and high values were observed between Argentina and populations from two African countries (Morocco and Kenya), Australia, and Hawaii (Kauai). Pest introduction sources and dispersion patterns in Argentina are discussed based on these results as well as on available bibliographical data. © 2010 The Authors. Journal compilation © 2010 The Netherlands Entomological Society.
format JOUR
author Lanzavecchia, S.
Remis, M.
Cladera, J.
Zandomeni, R.
author_facet Lanzavecchia, S.
Remis, M.
Cladera, J.
Zandomeni, R.
author_sort Lanzavecchia, S.
title Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata
title_short Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata
title_full Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata
title_fullStr Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata
title_full_unstemmed Adh1 first intron polymorphisms in Argentinean populations of Ceratitis capitata
title_sort adh1 first intron polymorphisms in argentinean populations of ceratitis capitata
url http://hdl.handle.net/20.500.12110/paper_00138703_v136_n1_p53_Lanzavecchia
work_keys_str_mv AT lanzavecchias adh1firstintronpolymorphismsinargentineanpopulationsofceratitiscapitata
AT remism adh1firstintronpolymorphismsinargentineanpopulationsofceratitiscapitata
AT claderaj adh1firstintronpolymorphismsinargentineanpopulationsofceratitiscapitata
AT zandomenir adh1firstintronpolymorphismsinargentineanpopulationsofceratitiscapitata
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