Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse

Glutamic acid decarboxylase (GAD), among other potential autoantigens, is thought to play a crucial role in type I diabetes, particularly in a spontaneous model of the disease, the nonobese diabetic (NOD) mouse. In the pancreas, the presence of GAD and γ-aminobutyric acid (GABA), the decarboxylation...

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Autores principales: Saravia-Fernandez, F., Faveeuw, C., Blasquez-Bulant, C., Tappaz, M., Throsby, M., Pelletier, G., Vaudry, H., Dardenne, M., Homo-Delarche, F.
Formato: JOUR
Materias:
4 aminobutyric acid
glutamate decarboxylase
neuropeptide Y
animal model
animal tissue
article
controlled study
diabetes mellitus
enzyme analysis
enzyme localization
immunohistochemistry
insulitis
mouse
nonhuman
pancreas
priority journal
T lymphocyte
tissue distribution
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00137227_v137_n8_p3497_SaraviaFernandez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00137227_v137_n8_p3497_SaraviaFernandez
record_format dspace
spelling todo:paper_00137227_v137_n8_p3497_SaraviaFernandez2023-10-03T14:11:11Z Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse Saravia-Fernandez, F. Faveeuw, C. Blasquez-Bulant, C. Tappaz, M. Throsby, M. Pelletier, G. Vaudry, H. Dardenne, M. Homo-Delarche, F. 4 aminobutyric acid glutamate decarboxylase neuropeptide Y animal model animal tissue article controlled study diabetes mellitus enzyme analysis enzyme localization immunohistochemistry insulitis mouse nonhuman pancreas priority journal T lymphocyte tissue distribution Glutamic acid decarboxylase (GAD), among other potential autoantigens, is thought to play a crucial role in type I diabetes, particularly in a spontaneous model of the disease, the nonobese diabetic (NOD) mouse. In the pancreas, the presence of GAD and γ-aminobutyric acid (GABA), the decarboxylation product of GAD and a putative neurotransmitter in the islets of Langerhans, is well documented in the β-cells. This is particularly true in rats, in which another GABAergic structure exists near the islets, the neuronal bodies. In this study, first the GABA content was measured in isolated islets from NOD and C57BL/6 mice (controls), and a decrease was found in NOD females as their insulitis progressed. Second, for the first time in mice, confocal analysis of immunofluorescent-labeled pancreatic sections revealed near the islets neuronal structures in which GAD and neuropeptide Y were colocalized, as they are in the brain. These structures were always observed in the pancreata of both sexes of C57BL/6 mice at the various ages investigated. In NOD mice, however, these neuronal structures were only detected in young females (< 10 weeks old) and in males until an intermediate age. Moreover, patches oft cells surrounding GAD-containing fibers were seen in the vicinity of the islets with incipient periinsulitis. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00137227_v137_n8_p3497_SaraviaFernandez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 4 aminobutyric acid
glutamate decarboxylase
neuropeptide Y
animal model
animal tissue
article
controlled study
diabetes mellitus
enzyme analysis
enzyme localization
immunohistochemistry
insulitis
mouse
nonhuman
pancreas
priority journal
T lymphocyte
tissue distribution
spellingShingle 4 aminobutyric acid
glutamate decarboxylase
neuropeptide Y
animal model
animal tissue
article
controlled study
diabetes mellitus
enzyme analysis
enzyme localization
immunohistochemistry
insulitis
mouse
nonhuman
pancreas
priority journal
T lymphocyte
tissue distribution
Saravia-Fernandez, F.
Faveeuw, C.
Blasquez-Bulant, C.
Tappaz, M.
Throsby, M.
Pelletier, G.
Vaudry, H.
Dardenne, M.
Homo-Delarche, F.
Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse
topic_facet 4 aminobutyric acid
glutamate decarboxylase
neuropeptide Y
animal model
animal tissue
article
controlled study
diabetes mellitus
enzyme analysis
enzyme localization
immunohistochemistry
insulitis
mouse
nonhuman
pancreas
priority journal
T lymphocyte
tissue distribution
description Glutamic acid decarboxylase (GAD), among other potential autoantigens, is thought to play a crucial role in type I diabetes, particularly in a spontaneous model of the disease, the nonobese diabetic (NOD) mouse. In the pancreas, the presence of GAD and γ-aminobutyric acid (GABA), the decarboxylation product of GAD and a putative neurotransmitter in the islets of Langerhans, is well documented in the β-cells. This is particularly true in rats, in which another GABAergic structure exists near the islets, the neuronal bodies. In this study, first the GABA content was measured in isolated islets from NOD and C57BL/6 mice (controls), and a decrease was found in NOD females as their insulitis progressed. Second, for the first time in mice, confocal analysis of immunofluorescent-labeled pancreatic sections revealed near the islets neuronal structures in which GAD and neuropeptide Y were colocalized, as they are in the brain. These structures were always observed in the pancreata of both sexes of C57BL/6 mice at the various ages investigated. In NOD mice, however, these neuronal structures were only detected in young females (< 10 weeks old) and in males until an intermediate age. Moreover, patches oft cells surrounding GAD-containing fibers were seen in the vicinity of the islets with incipient periinsulitis.
format JOUR
author Saravia-Fernandez, F.
Faveeuw, C.
Blasquez-Bulant, C.
Tappaz, M.
Throsby, M.
Pelletier, G.
Vaudry, H.
Dardenne, M.
Homo-Delarche, F.
author_facet Saravia-Fernandez, F.
Faveeuw, C.
Blasquez-Bulant, C.
Tappaz, M.
Throsby, M.
Pelletier, G.
Vaudry, H.
Dardenne, M.
Homo-Delarche, F.
author_sort Saravia-Fernandez, F.
title Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse
title_short Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse
title_full Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse
title_fullStr Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse
title_full_unstemmed Localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse
title_sort localization of γ-aminobutyric acid and glutamic acid decarboxylase in the pancreas of the nonobese diabetic mouse
url http://hdl.handle.net/20.500.12110/paper_00137227_v137_n8_p3497_SaraviaFernandez
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