An Os(byp)2ClPyCH2NHPoly(allylamine) hydrogel mediator for enzyme wiring at electrodes

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An Os hydrogel based on the covalent attachment of Os(byp)2 ClPyCHO to PAA-NH2 was cross-linked with glucose oxidase (GOx), lactate oxidase (LOx) and horseradish peroxidase (HRP) on GC and Au electrodes by PEG-400 bifunctional reagent. Single layer monoenzyme (GOx or LOx) and bienzyme (HRP-GOx) sing...

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Autores principales: Danilowicz, C., Cortón, E., Battaglini, F., Calvo, E.J.
Formato: JOUR
Materias:
Biosensor
Enzyme electrodes
FIA
GOx
HRP
LOx
Os polymer
Amperometric sensors
Catalysis
Crosslinking
Cyclic voltammetry
Diffusion
Electrolytes
Enzymes
Glucose
Hydrogels
Oxidation
Polymers
Substrates
Electron shuttles
Electron wires
Enzyme electrode
Glucose oxidase
Horseradish peroxidase
Hydrodynamic electrode
Lactate oxidase
Rotating disc electrode
Wall jet electrode
Electrochemical electrodes
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00134686_v43_n23_p3525_Danilowicz
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00134686_v43_n23_p3525_Danilowicz
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spelling todo:paper_00134686_v43_n23_p3525_Danilowicz2023-10-03T14:10:57Z An Os(byp)2ClPyCH2NHPoly(allylamine) hydrogel mediator for enzyme wiring at electrodes Danilowicz, C. Cortón, E. Battaglini, F. Calvo, E.J. Biosensor Enzyme electrodes FIA GOx HRP LOx Os polymer Amperometric sensors Catalysis Crosslinking Cyclic voltammetry Diffusion Electrolytes Enzymes Glucose Hydrogels Oxidation Polymers Substrates Electron shuttles Electron wires Enzyme electrode Glucose oxidase Horseradish peroxidase Hydrodynamic electrode Lactate oxidase Rotating disc electrode Wall jet electrode Electrochemical electrodes An Os hydrogel based on the covalent attachment of Os(byp)2 ClPyCHO to PAA-NH2 was cross-linked with glucose oxidase (GOx), lactate oxidase (LOx) and horseradish peroxidase (HRP) on GC and Au electrodes by PEG-400 bifunctional reagent. Single layer monoenzyme (GOx or LOx) and bienzyme (HRP-GOx) single layer modified electrodes were prepared with the Os moieties acting as "electron wires or electron shuttles". Cyclic voltammetry showed diffusional charge propagation in the gel which resulted more stable than similar ferrocene based gels reported before. In solutions containing the substrates, catalytic currents were obtained due to enzyme catalysis for the oxidation of glucose and lactate by the respective enzymes mediated by the Os polymer either by detecting directly the anodic current in a single enzyme electrode or indirectly by further reducing the peroxide formed in the aerobic enzymatic cycle at the Os-wired HRP. A rotating disc electrode (RDE) and a wall jet electrode (WJE) were employed as hydrodynamic electrodes in order to correct the amperometric response for substrate concentration polarization in the external electrolyte. © 1998 Elsevier Science Ltd. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00134686_v43_n23_p3525_Danilowicz
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Biosensor
Enzyme electrodes
FIA
GOx
HRP
LOx
Os polymer
Amperometric sensors
Catalysis
Crosslinking
Cyclic voltammetry
Diffusion
Electrolytes
Enzymes
Glucose
Hydrogels
Oxidation
Polymers
Substrates
Electron shuttles
Electron wires
Enzyme electrode
Glucose oxidase
Horseradish peroxidase
Hydrodynamic electrode
Lactate oxidase
Rotating disc electrode
Wall jet electrode
Electrochemical electrodes
spellingShingle Biosensor
Enzyme electrodes
FIA
GOx
HRP
LOx
Os polymer
Amperometric sensors
Catalysis
Crosslinking
Cyclic voltammetry
Diffusion
Electrolytes
Enzymes
Glucose
Hydrogels
Oxidation
Polymers
Substrates
Electron shuttles
Electron wires
Enzyme electrode
Glucose oxidase
Horseradish peroxidase
Hydrodynamic electrode
Lactate oxidase
Rotating disc electrode
Wall jet electrode
Electrochemical electrodes
Danilowicz, C.
Cortón, E.
Battaglini, F.
Calvo, E.J.
An Os(byp)2ClPyCH2NHPoly(allylamine) hydrogel mediator for enzyme wiring at electrodes
topic_facet Biosensor
Enzyme electrodes
FIA
GOx
HRP
LOx
Os polymer
Amperometric sensors
Catalysis
Crosslinking
Cyclic voltammetry
Diffusion
Electrolytes
Enzymes
Glucose
Hydrogels
Oxidation
Polymers
Substrates
Electron shuttles
Electron wires
Enzyme electrode
Glucose oxidase
Horseradish peroxidase
Hydrodynamic electrode
Lactate oxidase
Rotating disc electrode
Wall jet electrode
Electrochemical electrodes
description An Os hydrogel based on the covalent attachment of Os(byp)2 ClPyCHO to PAA-NH2 was cross-linked with glucose oxidase (GOx), lactate oxidase (LOx) and horseradish peroxidase (HRP) on GC and Au electrodes by PEG-400 bifunctional reagent. Single layer monoenzyme (GOx or LOx) and bienzyme (HRP-GOx) single layer modified electrodes were prepared with the Os moieties acting as "electron wires or electron shuttles". Cyclic voltammetry showed diffusional charge propagation in the gel which resulted more stable than similar ferrocene based gels reported before. In solutions containing the substrates, catalytic currents were obtained due to enzyme catalysis for the oxidation of glucose and lactate by the respective enzymes mediated by the Os polymer either by detecting directly the anodic current in a single enzyme electrode or indirectly by further reducing the peroxide formed in the aerobic enzymatic cycle at the Os-wired HRP. A rotating disc electrode (RDE) and a wall jet electrode (WJE) were employed as hydrodynamic electrodes in order to correct the amperometric response for substrate concentration polarization in the external electrolyte. © 1998 Elsevier Science Ltd. All rights reserved.
format JOUR
author Danilowicz, C.
Cortón, E.
Battaglini, F.
Calvo, E.J.
author_facet Danilowicz, C.
Cortón, E.
Battaglini, F.
Calvo, E.J.
author_sort Danilowicz, C.
title An Os(byp)2ClPyCH2NHPoly(allylamine) hydrogel mediator for enzyme wiring at electrodes
title_short An Os(byp)2ClPyCH2NHPoly(allylamine) hydrogel mediator for enzyme wiring at electrodes
title_full An Os(byp)2ClPyCH2NHPoly(allylamine) hydrogel mediator for enzyme wiring at electrodes
title_fullStr An Os(byp)2ClPyCH2NHPoly(allylamine) hydrogel mediator for enzyme wiring at electrodes
title_full_unstemmed An Os(byp)2ClPyCH2NHPoly(allylamine) hydrogel mediator for enzyme wiring at electrodes
title_sort os(byp)2clpych2nhpoly(allylamine) hydrogel mediator for enzyme wiring at electrodes
url http://hdl.handle.net/20.500.12110/paper_00134686_v43_n23_p3525_Danilowicz
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AT cortone anosbyp2clpych2nhpolyallylaminehydrogelmediatorforenzymewiringatelectrodes
AT battaglinif anosbyp2clpych2nhpolyallylaminehydrogelmediatorforenzymewiringatelectrodes
AT calvoej anosbyp2clpych2nhpolyallylaminehydrogelmediatorforenzymewiringatelectrodes
AT danilowiczc osbyp2clpych2nhpolyallylaminehydrogelmediatorforenzymewiringatelectrodes
AT cortone osbyp2clpych2nhpolyallylaminehydrogelmediatorforenzymewiringatelectrodes
AT battaglinif osbyp2clpych2nhpolyallylaminehydrogelmediatorforenzymewiringatelectrodes
AT calvoej osbyp2clpych2nhpolyallylaminehydrogelmediatorforenzymewiringatelectrodes
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