Immunoassay with recombinant Trypanosoma cruzi antigens potentially useful for screening donated blood and diagnosing Chagas disease

A new enzyme immunoassay (EIA), the Dia Kit(TM) Bio-Chagas assay (Gador S.A.), is potentially useful for detecting antibodies to Trypanosoma cruzi in the diagnosis of infected individuals and the screening of blood in blood banks. The EIA is carried out on test strips of plastic backing covered with...

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Autores principales:	Pastini, A.C., Iglesias, S.R., Carricarte, V.C., Guerin, M.E., Sanchez, D.O., Frasch, A.C.
Formato:	JOUR
Materias:	acute phase protein glutathione transferase hybrid protein immunoglobulin g protozoon antibody acute phase response adsorption article blood donor chagas disease controlled study diagnostic accuracy enzyme immunoassay enzyme linked immunosorbent assay hemagglutination human human tissue immunofluorescence screening test serology trypanosoma cruzi visceral leishmaniasis Adsorption Animal Antibodies, Protozoan Antigens, Protozoan beta-Galactosidase Chagas Disease Cloning, Molecular Comparative Study Human Immunoenzyme Techniques Reagent Kits, Diagnostic Recombinant Fusion Proteins Recombinant Proteins Sensitivity and Specificity Support, Non-U.S. Gov't Trypanosoma cruzi Trypanosoma
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00099147_v40_n10_p1893_Pastini
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00099147_v40_n10_p1893_Pastini
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spelling	todo:paper_00099147_v40_n10_p1893_Pastini2023-10-03T14:08:53Z Immunoassay with recombinant Trypanosoma cruzi antigens potentially useful for screening donated blood and diagnosing Chagas disease Pastini, A.C. Iglesias, S.R. Carricarte, V.C. Guerin, M.E. Sanchez, D.O. Frasch, A.C. acute phase protein glutathione transferase hybrid protein immunoglobulin g protozoon antibody acute phase response adsorption article blood donor chagas disease controlled study diagnostic accuracy enzyme immunoassay enzyme linked immunosorbent assay hemagglutination human human tissue immunofluorescence screening test serology trypanosoma cruzi visceral leishmaniasis Adsorption Animal Antibodies, Protozoan Antigens, Protozoan beta-Galactosidase Chagas Disease Cloning, Molecular Comparative Study Human Immunoenzyme Techniques Reagent Kits, Diagnostic Recombinant Fusion Proteins Recombinant Proteins Sensitivity and Specificity Support, Non-U.S. Gov't Trypanosoma cruzi Trypanosoma Trypanosoma cruzi A new enzyme immunoassay (EIA), the Dia Kit(TM) Bio-Chagas assay (Gador S.A.), is potentially useful for detecting antibodies to Trypanosoma cruzi in the diagnosis of infected individuals and the screening of blood in blood banks. The EIA is carried out on test strips of plastic backing covered with a nitrocellulose membrane to which a mixture of recombinant T. cruzi antigens 1, 2, shed acute-phase antigen, 13, and 30 has been applied as a horizontal line. A horizontal line of human IgG is included to monitor the test procedure. The test strips exhibited homogeneity in the adsorption of the mixture of recombinant antigens (CV = 6.0%) and the human IgG (CV = 8.6%). The EIA results obtained with sera positive by xenodiagnosis showed 100% agreement between both types of tests; tested against sera with positive and negative matched results of indirect hemagglutination, immunofluorescence, and ELISA, the EIA results agreed for 99.1% (347 of 350) and 99.6% (299 of 300) of the samples, respectively. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00099147_v40_n10_p1893_Pastini
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	acute phase protein glutathione transferase hybrid protein immunoglobulin g protozoon antibody acute phase response adsorption article blood donor chagas disease controlled study diagnostic accuracy enzyme immunoassay enzyme linked immunosorbent assay hemagglutination human human tissue immunofluorescence screening test serology trypanosoma cruzi visceral leishmaniasis Adsorption Animal Antibodies, Protozoan Antigens, Protozoan beta-Galactosidase Chagas Disease Cloning, Molecular Comparative Study Human Immunoenzyme Techniques Reagent Kits, Diagnostic Recombinant Fusion Proteins Recombinant Proteins Sensitivity and Specificity Support, Non-U.S. Gov't Trypanosoma cruzi Trypanosoma Trypanosoma cruzi
spellingShingle	acute phase protein glutathione transferase hybrid protein immunoglobulin g protozoon antibody acute phase response adsorption article blood donor chagas disease controlled study diagnostic accuracy enzyme immunoassay enzyme linked immunosorbent assay hemagglutination human human tissue immunofluorescence screening test serology trypanosoma cruzi visceral leishmaniasis Adsorption Animal Antibodies, Protozoan Antigens, Protozoan beta-Galactosidase Chagas Disease Cloning, Molecular Comparative Study Human Immunoenzyme Techniques Reagent Kits, Diagnostic Recombinant Fusion Proteins Recombinant Proteins Sensitivity and Specificity Support, Non-U.S. Gov't Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Pastini, A.C. Iglesias, S.R. Carricarte, V.C. Guerin, M.E. Sanchez, D.O. Frasch, A.C. Immunoassay with recombinant Trypanosoma cruzi antigens potentially useful for screening donated blood and diagnosing Chagas disease
topic_facet	acute phase protein glutathione transferase hybrid protein immunoglobulin g protozoon antibody acute phase response adsorption article blood donor chagas disease controlled study diagnostic accuracy enzyme immunoassay enzyme linked immunosorbent assay hemagglutination human human tissue immunofluorescence screening test serology trypanosoma cruzi visceral leishmaniasis Adsorption Animal Antibodies, Protozoan Antigens, Protozoan beta-Galactosidase Chagas Disease Cloning, Molecular Comparative Study Human Immunoenzyme Techniques Reagent Kits, Diagnostic Recombinant Fusion Proteins Recombinant Proteins Sensitivity and Specificity Support, Non-U.S. Gov't Trypanosoma cruzi Trypanosoma Trypanosoma cruzi
description	A new enzyme immunoassay (EIA), the Dia Kit(TM) Bio-Chagas assay (Gador S.A.), is potentially useful for detecting antibodies to Trypanosoma cruzi in the diagnosis of infected individuals and the screening of blood in blood banks. The EIA is carried out on test strips of plastic backing covered with a nitrocellulose membrane to which a mixture of recombinant T. cruzi antigens 1, 2, shed acute-phase antigen, 13, and 30 has been applied as a horizontal line. A horizontal line of human IgG is included to monitor the test procedure. The test strips exhibited homogeneity in the adsorption of the mixture of recombinant antigens (CV = 6.0%) and the human IgG (CV = 8.6%). The EIA results obtained with sera positive by xenodiagnosis showed 100% agreement between both types of tests; tested against sera with positive and negative matched results of indirect hemagglutination, immunofluorescence, and ELISA, the EIA results agreed for 99.1% (347 of 350) and 99.6% (299 of 300) of the samples, respectively.
format	JOUR
author	Pastini, A.C. Iglesias, S.R. Carricarte, V.C. Guerin, M.E. Sanchez, D.O. Frasch, A.C.
author_facet	Pastini, A.C. Iglesias, S.R. Carricarte, V.C. Guerin, M.E. Sanchez, D.O. Frasch, A.C.
author_sort	Pastini, A.C.
title	Immunoassay with recombinant Trypanosoma cruzi antigens potentially useful for screening donated blood and diagnosing Chagas disease
title_short	Immunoassay with recombinant Trypanosoma cruzi antigens potentially useful for screening donated blood and diagnosing Chagas disease
title_full	Immunoassay with recombinant Trypanosoma cruzi antigens potentially useful for screening donated blood and diagnosing Chagas disease
title_fullStr	Immunoassay with recombinant Trypanosoma cruzi antigens potentially useful for screening donated blood and diagnosing Chagas disease
title_full_unstemmed	Immunoassay with recombinant Trypanosoma cruzi antigens potentially useful for screening donated blood and diagnosing Chagas disease
title_sort	immunoassay with recombinant trypanosoma cruzi antigens potentially useful for screening donated blood and diagnosing chagas disease
url	http://hdl.handle.net/20.500.12110/paper_00099147_v40_n10_p1893_Pastini
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Immunoassay with recombinant Trypanosoma cruzi antigens potentially useful for screening donated blood and diagnosing Chagas disease

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