Antibodies to β1 and β2 adrenoreceptors in Chagas' disease
Evidence accumulated over the last decade concerning human and experimental models suggests that an immunopathological mechanism may be involved in the pathogenesis of chronic Chagas' disease. In this paper we demonstrate the existence of two different circulating IgG in chagasic patients which...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00099104_v74_n3_p349_SterinBorda |
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todo:paper_00099104_v74_n3_p349_SterinBorda2023-10-03T14:08:50Z Antibodies to β1 and β2 adrenoreceptors in Chagas' disease Sterin-Borda, L. Perez Leiros, C. Wald, M. Cremaschi, G. Borda, E. beta 1 adrenergic receptor beta 2 adrenergic receptor cyclic amp dihydroalprenolol immunoglobulin g isoprenaline propranolol receptor antibody chagas disease clinical article human priority journal protozoon Antibody Specificity Chagas Cardiomyopathy Cyclic AMP Dihydroalprenolol Dose-Response Relationship, Immunologic Human Immunoglobulin G Immunoglobulins, Fab Lymphocytes Myocardium Receptors, Adrenergic, beta Spleen Time Factors Evidence accumulated over the last decade concerning human and experimental models suggests that an immunopathological mechanism may be involved in the pathogenesis of chronic Chagas' disease. In this paper we demonstrate the existence of two different circulating IgG in chagasic patients which bind with myocardial β1 and spleen cell β2 adrenoceptors, acting as non-competitive inhibitors. Both chagasic IgG against β1 and β2 adrenoceptor increased intracellular levels of cAMP that could be blocked by specific β1 and β2 adrenoceptor antagonist. The specificity for β1 and β2 adrenoceptors and the independence of other tissue reactive antibodies was demonstrated by IgG absorption with turkey red blood cell (TRBC), human lymphocytes (HL) or guinea pig red blood cells (GPRBC). The F(ab')2 fraction acted similarly. This supports the specificity of β1 and β2 adrenoceptors to the chagasic IgG and the independence of the other tissue reactive antibodies, such as EVI system. The probable pathogenic role of both β1 and β2 adrenergic chagasic antibody is discussed. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00099104_v74_n3_p349_SterinBorda |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
beta 1 adrenergic receptor beta 2 adrenergic receptor cyclic amp dihydroalprenolol immunoglobulin g isoprenaline propranolol receptor antibody chagas disease clinical article human priority journal protozoon Antibody Specificity Chagas Cardiomyopathy Cyclic AMP Dihydroalprenolol Dose-Response Relationship, Immunologic Human Immunoglobulin G Immunoglobulins, Fab Lymphocytes Myocardium Receptors, Adrenergic, beta Spleen Time Factors |
| spellingShingle |
beta 1 adrenergic receptor beta 2 adrenergic receptor cyclic amp dihydroalprenolol immunoglobulin g isoprenaline propranolol receptor antibody chagas disease clinical article human priority journal protozoon Antibody Specificity Chagas Cardiomyopathy Cyclic AMP Dihydroalprenolol Dose-Response Relationship, Immunologic Human Immunoglobulin G Immunoglobulins, Fab Lymphocytes Myocardium Receptors, Adrenergic, beta Spleen Time Factors Sterin-Borda, L. Perez Leiros, C. Wald, M. Cremaschi, G. Borda, E. Antibodies to β1 and β2 adrenoreceptors in Chagas' disease |
| topic_facet |
beta 1 adrenergic receptor beta 2 adrenergic receptor cyclic amp dihydroalprenolol immunoglobulin g isoprenaline propranolol receptor antibody chagas disease clinical article human priority journal protozoon Antibody Specificity Chagas Cardiomyopathy Cyclic AMP Dihydroalprenolol Dose-Response Relationship, Immunologic Human Immunoglobulin G Immunoglobulins, Fab Lymphocytes Myocardium Receptors, Adrenergic, beta Spleen Time Factors |
| description |
Evidence accumulated over the last decade concerning human and experimental models suggests that an immunopathological mechanism may be involved in the pathogenesis of chronic Chagas' disease. In this paper we demonstrate the existence of two different circulating IgG in chagasic patients which bind with myocardial β1 and spleen cell β2 adrenoceptors, acting as non-competitive inhibitors. Both chagasic IgG against β1 and β2 adrenoceptor increased intracellular levels of cAMP that could be blocked by specific β1 and β2 adrenoceptor antagonist. The specificity for β1 and β2 adrenoceptors and the independence of other tissue reactive antibodies was demonstrated by IgG absorption with turkey red blood cell (TRBC), human lymphocytes (HL) or guinea pig red blood cells (GPRBC). The F(ab')2 fraction acted similarly. This supports the specificity of β1 and β2 adrenoceptors to the chagasic IgG and the independence of the other tissue reactive antibodies, such as EVI system. The probable pathogenic role of both β1 and β2 adrenergic chagasic antibody is discussed. |
| format |
JOUR |
| author |
Sterin-Borda, L. Perez Leiros, C. Wald, M. Cremaschi, G. Borda, E. |
| author_facet |
Sterin-Borda, L. Perez Leiros, C. Wald, M. Cremaschi, G. Borda, E. |
| author_sort |
Sterin-Borda, L. |
| title |
Antibodies to β1 and β2 adrenoreceptors in Chagas' disease |
| title_short |
Antibodies to β1 and β2 adrenoreceptors in Chagas' disease |
| title_full |
Antibodies to β1 and β2 adrenoreceptors in Chagas' disease |
| title_fullStr |
Antibodies to β1 and β2 adrenoreceptors in Chagas' disease |
| title_full_unstemmed |
Antibodies to β1 and β2 adrenoreceptors in Chagas' disease |
| title_sort |
antibodies to β1 and β2 adrenoreceptors in chagas' disease |
| url |
http://hdl.handle.net/20.500.12110/paper_00099104_v74_n3_p349_SterinBorda |
| work_keys_str_mv |
AT sterinbordal antibodiestob1andb2adrenoreceptorsinchagasdisease AT perezleirosc antibodiestob1andb2adrenoreceptorsinchagasdisease AT waldm antibodiestob1andb2adrenoreceptorsinchagasdisease AT cremaschig antibodiestob1andb2adrenoreceptorsinchagasdisease AT bordae antibodiestob1andb2adrenoreceptorsinchagasdisease |
| _version_ |
1807318869882175488 |