Macrocycle versus podant-type neutral ionophore in potentiometric detection of mucolytic agents following separation by various HPLC modes
Potentiometric detection of lipophilic drugs such as clenbuterol, ambroxol and bromhexine in standard-bore cyano and narrow-bore hybrid, reversed-phase HPLC systems was developed to enable specific determination of these mucolytic agents in some marketed oral-formulations. The macrocycle and podant-...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00095893_v57_n11-12_p757_Bazylak |
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todo:paper_00095893_v57_n11-12_p757_Bazylak2023-10-03T14:08:39Z Macrocycle versus podant-type neutral ionophore in potentiometric detection of mucolytic agents following separation by various HPLC modes Bazylak, G. Nagels, L.J. Monge, M.E. Column liquid chromatography Lipophilic drugs Pharmaceutical formulations Potentiometric detection acetonitrile ambroxol bromhexine clenbuterol hexakis(2,3,6,tri o octyl) alpha cyclodextrin ionophore macrocyclic compound mucolytic agent n,n,n'n' tetracyclohexyloxybis(2 phenyleneoxy)diacetamide polyvinylchloride unclassified drug acidity aqueous solution article electrode lipophilicity liquid membrane potentiometry priority journal reversed phase high performance liquid chromatography separation technique signal noise ratio standardization ultraviolet radiation waveform Potentiometric detection of lipophilic drugs such as clenbuterol, ambroxol and bromhexine in standard-bore cyano and narrow-bore hybrid, reversed-phase HPLC systems was developed to enable specific determination of these mucolytic agents in some marketed oral-formulations. The macrocycle and podant-type, neutral ionophores, respectively: hexakis(2,3,6-tri-O-octyl)-α-cyclodextrin (CDX) and N,N,N′,N′-tetracyclohexyl-oxybis(o-phenyleneoxy)diacetamide (TOPA), were used to construct PVC-based, liquid membrane electrodes. In acidic aqueous-acetonitrile, mobile phases both ionophores improved the sensitivity of these electrodes for mucolytic agents when compared to single-wavelength UV detection - as well as with the ionophore-free based electrode. In the case of bromhexine, indicating highest lipophilicity (logP 6.40), detection limits of 2.6 × 10-10 and 1.4 × -10 M (injected concentration) were achieved with a signal-to-noise (S/N) ratio of 3 with the TOPA-modified, liquid membrane electrode in the variaus RP-HPLC systems. The results of procedures validated for simultaneous potentiometric determination of clenbuterol and ambroxol in tablets marketed in different countries are also presented. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00095893_v57_n11-12_p757_Bazylak |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Column liquid chromatography Lipophilic drugs Pharmaceutical formulations Potentiometric detection acetonitrile ambroxol bromhexine clenbuterol hexakis(2,3,6,tri o octyl) alpha cyclodextrin ionophore macrocyclic compound mucolytic agent n,n,n'n' tetracyclohexyloxybis(2 phenyleneoxy)diacetamide polyvinylchloride unclassified drug acidity aqueous solution article electrode lipophilicity liquid membrane potentiometry priority journal reversed phase high performance liquid chromatography separation technique signal noise ratio standardization ultraviolet radiation waveform |
| spellingShingle |
Column liquid chromatography Lipophilic drugs Pharmaceutical formulations Potentiometric detection acetonitrile ambroxol bromhexine clenbuterol hexakis(2,3,6,tri o octyl) alpha cyclodextrin ionophore macrocyclic compound mucolytic agent n,n,n'n' tetracyclohexyloxybis(2 phenyleneoxy)diacetamide polyvinylchloride unclassified drug acidity aqueous solution article electrode lipophilicity liquid membrane potentiometry priority journal reversed phase high performance liquid chromatography separation technique signal noise ratio standardization ultraviolet radiation waveform Bazylak, G. Nagels, L.J. Monge, M.E. Macrocycle versus podant-type neutral ionophore in potentiometric detection of mucolytic agents following separation by various HPLC modes |
| topic_facet |
Column liquid chromatography Lipophilic drugs Pharmaceutical formulations Potentiometric detection acetonitrile ambroxol bromhexine clenbuterol hexakis(2,3,6,tri o octyl) alpha cyclodextrin ionophore macrocyclic compound mucolytic agent n,n,n'n' tetracyclohexyloxybis(2 phenyleneoxy)diacetamide polyvinylchloride unclassified drug acidity aqueous solution article electrode lipophilicity liquid membrane potentiometry priority journal reversed phase high performance liquid chromatography separation technique signal noise ratio standardization ultraviolet radiation waveform |
| description |
Potentiometric detection of lipophilic drugs such as clenbuterol, ambroxol and bromhexine in standard-bore cyano and narrow-bore hybrid, reversed-phase HPLC systems was developed to enable specific determination of these mucolytic agents in some marketed oral-formulations. The macrocycle and podant-type, neutral ionophores, respectively: hexakis(2,3,6-tri-O-octyl)-α-cyclodextrin (CDX) and N,N,N′,N′-tetracyclohexyl-oxybis(o-phenyleneoxy)diacetamide (TOPA), were used to construct PVC-based, liquid membrane electrodes. In acidic aqueous-acetonitrile, mobile phases both ionophores improved the sensitivity of these electrodes for mucolytic agents when compared to single-wavelength UV detection - as well as with the ionophore-free based electrode. In the case of bromhexine, indicating highest lipophilicity (logP 6.40), detection limits of 2.6 × 10-10 and 1.4 × -10 M (injected concentration) were achieved with a signal-to-noise (S/N) ratio of 3 with the TOPA-modified, liquid membrane electrode in the variaus RP-HPLC systems. The results of procedures validated for simultaneous potentiometric determination of clenbuterol and ambroxol in tablets marketed in different countries are also presented. |
| format |
JOUR |
| author |
Bazylak, G. Nagels, L.J. Monge, M.E. |
| author_facet |
Bazylak, G. Nagels, L.J. Monge, M.E. |
| author_sort |
Bazylak, G. |
| title |
Macrocycle versus podant-type neutral ionophore in potentiometric detection of mucolytic agents following separation by various HPLC modes |
| title_short |
Macrocycle versus podant-type neutral ionophore in potentiometric detection of mucolytic agents following separation by various HPLC modes |
| title_full |
Macrocycle versus podant-type neutral ionophore in potentiometric detection of mucolytic agents following separation by various HPLC modes |
| title_fullStr |
Macrocycle versus podant-type neutral ionophore in potentiometric detection of mucolytic agents following separation by various HPLC modes |
| title_full_unstemmed |
Macrocycle versus podant-type neutral ionophore in potentiometric detection of mucolytic agents following separation by various HPLC modes |
| title_sort |
macrocycle versus podant-type neutral ionophore in potentiometric detection of mucolytic agents following separation by various hplc modes |
| url |
http://hdl.handle.net/20.500.12110/paper_00095893_v57_n11-12_p757_Bazylak |
| work_keys_str_mv |
AT bazylakg macrocycleversuspodanttypeneutralionophoreinpotentiometricdetectionofmucolyticagentsfollowingseparationbyvarioushplcmodes AT nagelslj macrocycleversuspodanttypeneutralionophoreinpotentiometricdetectionofmucolyticagentsfollowingseparationbyvarioushplcmodes AT mongeme macrocycleversuspodanttypeneutralionophoreinpotentiometricdetectionofmucolyticagentsfollowingseparationbyvarioushplcmodes |
| _version_ |
1782030830031339520 |