Fluorescent banding and meiotic behaviour in erythrodiplax nigricans (libellulidae) and coryphaeschna perrensi (aeschnidae) (anisoptera, odonata)

The species of Odonata are cytogenetically characterised by possessing holokinetic chromosomes, a post-reductional meiosis, an XX/X0 (female/male) sex chromosome mechanism, m-chromosomes, and only one chiasma per bivalent. Chromosome studies were performed on males of Erythrodiplax nigricans and Cor...

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Autores principales: De Diego, G., Rebagliati, P.J., Mola, L.M.
Formato: JOUR
Materias:
Coryphaeschna perrensi
DAPI–CMA banding
Erythrodiplax nigricans
Heterochromatin
Holokinetic chromosomes
Meiosis
Odonata
Aeshnidae
Anisoptera (dragonflies)
Libellulidae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00087114_v61_n1_p60_DeDiego
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_00087114_v61_n1_p60_DeDiego2023-10-03T14:07:53Z Fluorescent banding and meiotic behaviour in erythrodiplax nigricans (libellulidae) and coryphaeschna perrensi (aeschnidae) (anisoptera, odonata) De Diego, G. Rebagliati, P.J. Mola, L.M. Coryphaeschna perrensi DAPI–CMA banding Erythrodiplax nigricans Heterochromatin Holokinetic chromosomes Meiosis Odonata Aeshnidae Anisoptera (dragonflies) Coryphaeschna perrensi Erythrodiplax nigricans Libellulidae Odonata The species of Odonata are cytogenetically characterised by possessing holokinetic chromosomes, a post-reductional meiosis, an XX/X0 (female/male) sex chromosome mechanism, m-chromosomes, and only one chiasma per bivalent. Chromosome studies were performed on males of Erythrodiplax nigricans and Coryphaeschna perrensi from Argentina. Erythrodiplax nigricans has n=12+XO and lacks m-chromosomes, while C. perrensi has 2n=27, n=13+XO, m-chromosomes and a large autosomal pair associated with the nucleolus. The meiotic behaviour of both species follows the general pattern of the order: the X chromosome is positively heteropycnotic during early prophase I; bivalents regularly show only one chiasma; all chromosomes migrate synchronously and almost parallel to the equatorial plane at anaphase I; at metaphase II the X chromosome is present in all the cells as a consequence of the post-reductional division, lies outside the metaphasic plate, and migrates asynchronously with the autosomes at anaphase II. In C. perrensi, the largest bivalent exhibits two chiasmata in a large proportion of cells, which is a very rare feature among dragonflies. Heterochromatin characterisation with DAPI-CMA banding reveals that C. perrensi does not show fluorescent banding, except for a CMA bright band at one telomeric region of the largest bivalent, associated with the NOR region; in E. nigricans, autosomes have small AT-rich telomeric blocks, except for the smallest pair, which exhibits conspicuous bands in both telomeric regions, one being GC-rich and the other AT-rich. Taking into account that the m-chromosomes have been found in other E. nigricans populations, their absence in the studied population may be due to the presence of such heterochromatic blocks. © 2008 Taylor & Francis Group, LLC. Fil:Rebagliati, P.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mola, L.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00087114_v61_n1_p60_DeDiego
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Coryphaeschna perrensi
DAPI–CMA banding
Erythrodiplax nigricans
Heterochromatin
Holokinetic chromosomes
Meiosis
Odonata
Aeshnidae
Anisoptera (dragonflies)
Coryphaeschna perrensi
Erythrodiplax nigricans
Libellulidae
Odonata
spellingShingle Coryphaeschna perrensi
DAPI–CMA banding
Erythrodiplax nigricans
Heterochromatin
Holokinetic chromosomes
Meiosis
Odonata
Aeshnidae
Anisoptera (dragonflies)
Coryphaeschna perrensi
Erythrodiplax nigricans
Libellulidae
Odonata
De Diego, G.
Rebagliati, P.J.
Mola, L.M.
Fluorescent banding and meiotic behaviour in erythrodiplax nigricans (libellulidae) and coryphaeschna perrensi (aeschnidae) (anisoptera, odonata)
topic_facet Coryphaeschna perrensi
DAPI–CMA banding
Erythrodiplax nigricans
Heterochromatin
Holokinetic chromosomes
Meiosis
Odonata
Aeshnidae
Anisoptera (dragonflies)
Coryphaeschna perrensi
Erythrodiplax nigricans
Libellulidae
Odonata
description The species of Odonata are cytogenetically characterised by possessing holokinetic chromosomes, a post-reductional meiosis, an XX/X0 (female/male) sex chromosome mechanism, m-chromosomes, and only one chiasma per bivalent. Chromosome studies were performed on males of Erythrodiplax nigricans and Coryphaeschna perrensi from Argentina. Erythrodiplax nigricans has n=12+XO and lacks m-chromosomes, while C. perrensi has 2n=27, n=13+XO, m-chromosomes and a large autosomal pair associated with the nucleolus. The meiotic behaviour of both species follows the general pattern of the order: the X chromosome is positively heteropycnotic during early prophase I; bivalents regularly show only one chiasma; all chromosomes migrate synchronously and almost parallel to the equatorial plane at anaphase I; at metaphase II the X chromosome is present in all the cells as a consequence of the post-reductional division, lies outside the metaphasic plate, and migrates asynchronously with the autosomes at anaphase II. In C. perrensi, the largest bivalent exhibits two chiasmata in a large proportion of cells, which is a very rare feature among dragonflies. Heterochromatin characterisation with DAPI-CMA banding reveals that C. perrensi does not show fluorescent banding, except for a CMA bright band at one telomeric region of the largest bivalent, associated with the NOR region; in E. nigricans, autosomes have small AT-rich telomeric blocks, except for the smallest pair, which exhibits conspicuous bands in both telomeric regions, one being GC-rich and the other AT-rich. Taking into account that the m-chromosomes have been found in other E. nigricans populations, their absence in the studied population may be due to the presence of such heterochromatic blocks. © 2008 Taylor & Francis Group, LLC.
format JOUR
author De Diego, G.
Rebagliati, P.J.
Mola, L.M.
author_facet De Diego, G.
Rebagliati, P.J.
Mola, L.M.
author_sort De Diego, G.
title Fluorescent banding and meiotic behaviour in erythrodiplax nigricans (libellulidae) and coryphaeschna perrensi (aeschnidae) (anisoptera, odonata)
title_short Fluorescent banding and meiotic behaviour in erythrodiplax nigricans (libellulidae) and coryphaeschna perrensi (aeschnidae) (anisoptera, odonata)
title_full Fluorescent banding and meiotic behaviour in erythrodiplax nigricans (libellulidae) and coryphaeschna perrensi (aeschnidae) (anisoptera, odonata)
title_fullStr Fluorescent banding and meiotic behaviour in erythrodiplax nigricans (libellulidae) and coryphaeschna perrensi (aeschnidae) (anisoptera, odonata)
title_full_unstemmed Fluorescent banding and meiotic behaviour in erythrodiplax nigricans (libellulidae) and coryphaeschna perrensi (aeschnidae) (anisoptera, odonata)
title_sort fluorescent banding and meiotic behaviour in erythrodiplax nigricans (libellulidae) and coryphaeschna perrensi (aeschnidae) (anisoptera, odonata)
url http://hdl.handle.net/20.500.12110/paper_00087114_v61_n1_p60_DeDiego
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AT rebagliatipj fluorescentbandingandmeioticbehaviourinerythrodiplaxnigricanslibellulidaeandcoryphaeschnaperrensiaeschnidaeanisopteraodonata
AT molalm fluorescentbandingandmeioticbehaviourinerythrodiplaxnigricanslibellulidaeandcoryphaeschnaperrensiaeschnidaeanisopteraodonata
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