Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales)

Polysiphonia nigrescens was sequentially extracted with water at room temperature, 70 and 90 °C. The extracts were analyzed and the major one, isolated at 70 °C, was fractionated by ion-exchange chromatography, eluting with water and solutions of increasing sodium chloride concentration; five main f...

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Autores principales:	Prado, H.J., Ciancia, M., Matulewicz, M.C.
Formato:	JOUR
Materias:	Agarans Algal polysaccharides Polysiphonia nigrescens Rhodomelaceae Structural analysis Sulfated galactans Algae Chromatographic analysis Ion exchange Methylation Nuclear magnetic resonance spectroscopy Sodium chloride Polysaccharides agaran agarose beta dextro xylose dimethyl ether hexamethonium bromide polysaccharide sodium chloride sulfate unclassified drug water xylose article carbon nuclear magnetic resonance ceramiales concentration (parameters) cyclization drug isolation elution extraction fractionation ion exchange chromatography methylation nuclear magnetic resonance nuclear magnetic resonance spectroscopy polysiphonia nigrescens priority journal proton nuclear magnetic resonance rhodomelaceae room temperature seaweed separation technique structure analysis Alkalies Cell Extracts Chromatography, Ion Exchange Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Seaweed Temperature algae Ceramiales
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00086215_v343_n4_p711_Prado
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00086215_v343_n4_p711_Prado
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spelling	todo:paper_00086215_v343_n4_p711_Prado2023-10-03T14:07:19Z Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales) Prado, H.J. Ciancia, M. Matulewicz, M.C. Agarans Algal polysaccharides Polysiphonia nigrescens Rhodomelaceae Structural analysis Sulfated galactans Algal polysaccharides Polysiphonia nigrescens Rhodomelaceae Sulfated galactans Algae Chromatographic analysis Ion exchange Methylation Nuclear magnetic resonance spectroscopy Sodium chloride Polysaccharides agaran agarose beta dextro xylose dimethyl ether hexamethonium bromide polysaccharide sodium chloride sulfate unclassified drug water xylose article carbon nuclear magnetic resonance ceramiales concentration (parameters) cyclization drug isolation elution extraction fractionation ion exchange chromatography methylation nuclear magnetic resonance nuclear magnetic resonance spectroscopy polysiphonia nigrescens priority journal proton nuclear magnetic resonance rhodomelaceae room temperature seaweed separation technique structure analysis Alkalies Cell Extracts Chromatography, Ion Exchange Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Polysaccharides Seaweed Temperature algae Ceramiales Polysiphonia nigrescens Rhodomelaceae Polysiphonia nigrescens was sequentially extracted with water at room temperature, 70 and 90 °C. The extracts were analyzed and the major one, isolated at 70 °C, was fractionated by ion-exchange chromatography, eluting with water and solutions of increasing sodium chloride concentration; five main fractions were separated. Structural analysis, carried out by methylation analysis and NMR spectroscopy, showed that four of these were partially cyclized agarans that were highly substituted on C-6 mainly with sulfate, although methyl ether and single stubs of β-d-xylose were found in minor proportions. A fifth fraction comprising 6-sulfated agarose was also isolated. The use of 2D NMR techniques allowed us to assign the 1H and 13C NMR resonances of the G6S→L6S diad for the first time. © 2008 Elsevier Ltd. All rights reserved. Fil:Prado, H.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ciancia, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00086215_v343_n4_p711_Prado
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Agarans Algal polysaccharides Polysiphonia nigrescens Rhodomelaceae Structural analysis Sulfated galactans Algal polysaccharides Polysiphonia nigrescens Rhodomelaceae Sulfated galactans Algae Chromatographic analysis Ion exchange Methylation Nuclear magnetic resonance spectroscopy Sodium chloride Polysaccharides agaran agarose beta dextro xylose dimethyl ether hexamethonium bromide polysaccharide sodium chloride sulfate unclassified drug water xylose article carbon nuclear magnetic resonance ceramiales concentration (parameters) cyclization drug isolation elution extraction fractionation ion exchange chromatography methylation nuclear magnetic resonance nuclear magnetic resonance spectroscopy polysiphonia nigrescens priority journal proton nuclear magnetic resonance rhodomelaceae room temperature seaweed separation technique structure analysis Alkalies Cell Extracts Chromatography, Ion Exchange Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Polysaccharides Seaweed Temperature algae Ceramiales Polysiphonia nigrescens Rhodomelaceae
spellingShingle	Agarans Algal polysaccharides Polysiphonia nigrescens Rhodomelaceae Structural analysis Sulfated galactans Algal polysaccharides Polysiphonia nigrescens Rhodomelaceae Sulfated galactans Algae Chromatographic analysis Ion exchange Methylation Nuclear magnetic resonance spectroscopy Sodium chloride Polysaccharides agaran agarose beta dextro xylose dimethyl ether hexamethonium bromide polysaccharide sodium chloride sulfate unclassified drug water xylose article carbon nuclear magnetic resonance ceramiales concentration (parameters) cyclization drug isolation elution extraction fractionation ion exchange chromatography methylation nuclear magnetic resonance nuclear magnetic resonance spectroscopy polysiphonia nigrescens priority journal proton nuclear magnetic resonance rhodomelaceae room temperature seaweed separation technique structure analysis Alkalies Cell Extracts Chromatography, Ion Exchange Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Polysaccharides Seaweed Temperature algae Ceramiales Polysiphonia nigrescens Rhodomelaceae Prado, H.J. Ciancia, M. Matulewicz, M.C. Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales)
topic_facet	Agarans Algal polysaccharides Polysiphonia nigrescens Rhodomelaceae Structural analysis Sulfated galactans Algal polysaccharides Polysiphonia nigrescens Rhodomelaceae Sulfated galactans Algae Chromatographic analysis Ion exchange Methylation Nuclear magnetic resonance spectroscopy Sodium chloride Polysaccharides agaran agarose beta dextro xylose dimethyl ether hexamethonium bromide polysaccharide sodium chloride sulfate unclassified drug water xylose article carbon nuclear magnetic resonance ceramiales concentration (parameters) cyclization drug isolation elution extraction fractionation ion exchange chromatography methylation nuclear magnetic resonance nuclear magnetic resonance spectroscopy polysiphonia nigrescens priority journal proton nuclear magnetic resonance rhodomelaceae room temperature seaweed separation technique structure analysis Alkalies Cell Extracts Chromatography, Ion Exchange Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Polysaccharides Seaweed Temperature algae Ceramiales Polysiphonia nigrescens Rhodomelaceae
description	Polysiphonia nigrescens was sequentially extracted with water at room temperature, 70 and 90 °C. The extracts were analyzed and the major one, isolated at 70 °C, was fractionated by ion-exchange chromatography, eluting with water and solutions of increasing sodium chloride concentration; five main fractions were separated. Structural analysis, carried out by methylation analysis and NMR spectroscopy, showed that four of these were partially cyclized agarans that were highly substituted on C-6 mainly with sulfate, although methyl ether and single stubs of β-d-xylose were found in minor proportions. A fifth fraction comprising 6-sulfated agarose was also isolated. The use of 2D NMR techniques allowed us to assign the 1H and 13C NMR resonances of the G6S→L6S diad for the first time. © 2008 Elsevier Ltd. All rights reserved.
format	JOUR
author	Prado, H.J. Ciancia, M. Matulewicz, M.C.
author_facet	Prado, H.J. Ciancia, M. Matulewicz, M.C.
author_sort	Prado, H.J.
title	Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales)
title_short	Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales)
title_full	Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales)
title_fullStr	Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales)
title_full_unstemmed	Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales)
title_sort	agarans from the red seaweed polysiphonia nigrescens (rhodomelaceae, ceramiales)
url	http://hdl.handle.net/20.500.12110/paper_00086215_v343_n4_p711_Prado
work_keys_str_mv	AT pradohj agaransfromtheredseaweedpolysiphonianigrescensrhodomelaceaeceramiales AT cianciam agaransfromtheredseaweedpolysiphonianigrescensrhodomelaceaeceramiales AT matulewiczmc agaransfromtheredseaweedpolysiphonianigrescensrhodomelaceaeceramiales
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Agarans from the red seaweed Polysiphonia nigrescens (Rhodomelaceae, Ceramiales)

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