Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris

In Xanthomonas campestris the genes involved in polysaccharide (xanthan) biosynthesis are located in a gene cluster (gum) of 16 kb. A Tn5 insertion mutant with a reduced slimy phenotype has been characterized. This mutant failed to produce the pentasaccharide repeating-unit of xanthan. Only three su...

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Autores principales:	Vojnov, A.A., Bassi, D.E., Daniels, M.J., Dankert, M.A.
Formato:	JOUR
Materias:	Biosynthesis Gum genes Polysaccharide Xanthan Cellulose Genes Glucose Lipids Phosphates Polymers Polysaccharides Sugars Mutant strains Wild type phenotypes Carbohydrates alkaline phosphatase cellulose edetic acid glucose glucuronic acid glycosyltransferase glycosyltransferase iv pentasaccharide polymer pyrophosphate trisaccharide unclassified drug xanthan article biosynthesis carbohydrate analysis catalysis controlled study gene cluster gene mutation nonhuman phenotype plasmid priority journal radioactivity structure analysis transposon Xanthomonas campestris Bacterial Proteins DNA Transposable Elements Glucuronic Acid Glycosyltransferases Lipopolysaccharides Oligosaccharides Polysaccharides, Bacterial insertion sequences transposons Xanthomonas
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00086215_v337_n4_p315_Vojnov
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00086215_v337_n4_p315_Vojnov2023-10-03T14:07:02Z Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris Vojnov, A.A. Bassi, D.E. Daniels, M.J. Dankert, M.A. Biosynthesis Gum genes Polysaccharide Xanthan Biosynthesis Cellulose Genes Glucose Lipids Phosphates Polymers Polysaccharides Sugars Mutant strains Wild type phenotypes Carbohydrates alkaline phosphatase cellulose edetic acid glucose glucuronic acid glycosyltransferase glycosyltransferase iv pentasaccharide polymer pyrophosphate trisaccharide unclassified drug xanthan article biosynthesis carbohydrate analysis catalysis controlled study gene cluster gene mutation nonhuman phenotype plasmid priority journal radioactivity structure analysis transposon Xanthomonas campestris Bacterial Proteins Cellulose DNA Transposable Elements Glucuronic Acid Glycosyltransferases Lipopolysaccharides Oligosaccharides Polysaccharides, Bacterial Xanthomonas campestris insertion sequences transposons Xanthomonas Xanthomonas campestris In Xanthomonas campestris the genes involved in polysaccharide (xanthan) biosynthesis are located in a gene cluster (gum) of 16 kb. A Tn5 insertion mutant with a reduced slimy phenotype has been characterized. This mutant failed to produce the pentasaccharide repeating-unit of xanthan. Only three sugars were transferred to the prenyl phosphate intermediate. Several lines of evidence suggested that the lipid-associated saccharide was the trisaccharide reducing end of the pentasaccharide from the wild-type strain. This trisaccharide was built up from UDP-Glc and GDP-Man, and a glucose residue was at the reducing end, linked to an allylic prenol through a diphosphate bridge. Results from one- or two-stage reactions showed that the trisaccharide-P-P-polyprenol was the precursor of the polymer. This new polymer, a polytrisaccharide, was detected also in vivo. The transposon responsible for the mutation was located within gumK gene. Therefore, this gene encodes for the glycosyltransferase IV, which catalyses the transfer of glucuronic acid to the lipid-linked β-D-Manp-(1 → 3)-β-D-Glcp-(1 → 4)-β-D-Glcp trisaccharide. A recombinant plasmid with the whole gum cluster restored the wild type phenotype. © 2002 Elsevier Science Ltd. All rights reserved. Fil:Vojnov, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bassi, D.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dankert, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00086215_v337_n4_p315_Vojnov
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Biosynthesis Gum genes Polysaccharide Xanthan Biosynthesis Cellulose Genes Glucose Lipids Phosphates Polymers Polysaccharides Sugars Mutant strains Wild type phenotypes Carbohydrates alkaline phosphatase cellulose edetic acid glucose glucuronic acid glycosyltransferase glycosyltransferase iv pentasaccharide polymer pyrophosphate trisaccharide unclassified drug xanthan article biosynthesis carbohydrate analysis catalysis controlled study gene cluster gene mutation nonhuman phenotype plasmid priority journal radioactivity structure analysis transposon Xanthomonas campestris Bacterial Proteins Cellulose DNA Transposable Elements Glucuronic Acid Glycosyltransferases Lipopolysaccharides Oligosaccharides Polysaccharides, Bacterial Xanthomonas campestris insertion sequences transposons Xanthomonas Xanthomonas campestris
spellingShingle	Biosynthesis Gum genes Polysaccharide Xanthan Biosynthesis Cellulose Genes Glucose Lipids Phosphates Polymers Polysaccharides Sugars Mutant strains Wild type phenotypes Carbohydrates alkaline phosphatase cellulose edetic acid glucose glucuronic acid glycosyltransferase glycosyltransferase iv pentasaccharide polymer pyrophosphate trisaccharide unclassified drug xanthan article biosynthesis carbohydrate analysis catalysis controlled study gene cluster gene mutation nonhuman phenotype plasmid priority journal radioactivity structure analysis transposon Xanthomonas campestris Bacterial Proteins Cellulose DNA Transposable Elements Glucuronic Acid Glycosyltransferases Lipopolysaccharides Oligosaccharides Polysaccharides, Bacterial Xanthomonas campestris insertion sequences transposons Xanthomonas Xanthomonas campestris Vojnov, A.A. Bassi, D.E. Daniels, M.J. Dankert, M.A. Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris
topic_facet	Biosynthesis Gum genes Polysaccharide Xanthan Biosynthesis Cellulose Genes Glucose Lipids Phosphates Polymers Polysaccharides Sugars Mutant strains Wild type phenotypes Carbohydrates alkaline phosphatase cellulose edetic acid glucose glucuronic acid glycosyltransferase glycosyltransferase iv pentasaccharide polymer pyrophosphate trisaccharide unclassified drug xanthan article biosynthesis carbohydrate analysis catalysis controlled study gene cluster gene mutation nonhuman phenotype plasmid priority journal radioactivity structure analysis transposon Xanthomonas campestris Bacterial Proteins Cellulose DNA Transposable Elements Glucuronic Acid Glycosyltransferases Lipopolysaccharides Oligosaccharides Polysaccharides, Bacterial Xanthomonas campestris insertion sequences transposons Xanthomonas Xanthomonas campestris
description	In Xanthomonas campestris the genes involved in polysaccharide (xanthan) biosynthesis are located in a gene cluster (gum) of 16 kb. A Tn5 insertion mutant with a reduced slimy phenotype has been characterized. This mutant failed to produce the pentasaccharide repeating-unit of xanthan. Only three sugars were transferred to the prenyl phosphate intermediate. Several lines of evidence suggested that the lipid-associated saccharide was the trisaccharide reducing end of the pentasaccharide from the wild-type strain. This trisaccharide was built up from UDP-Glc and GDP-Man, and a glucose residue was at the reducing end, linked to an allylic prenol through a diphosphate bridge. Results from one- or two-stage reactions showed that the trisaccharide-P-P-polyprenol was the precursor of the polymer. This new polymer, a polytrisaccharide, was detected also in vivo. The transposon responsible for the mutation was located within gumK gene. Therefore, this gene encodes for the glycosyltransferase IV, which catalyses the transfer of glucuronic acid to the lipid-linked β-D-Manp-(1 → 3)-β-D-Glcp-(1 → 4)-β-D-Glcp trisaccharide. A recombinant plasmid with the whole gum cluster restored the wild type phenotype. © 2002 Elsevier Science Ltd. All rights reserved.
format	JOUR
author	Vojnov, A.A. Bassi, D.E. Daniels, M.J. Dankert, M.A.
author_facet	Vojnov, A.A. Bassi, D.E. Daniels, M.J. Dankert, M.A.
author_sort	Vojnov, A.A.
title	Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris
title_short	Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris
title_full	Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris
title_fullStr	Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris
title_full_unstemmed	Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris
title_sort	biosynthesis of a substituted cellulose from a mutant strain of xanthomonas campestris
url	http://hdl.handle.net/20.500.12110/paper_00086215_v337_n4_p315_Vojnov
work_keys_str_mv	AT vojnovaa biosynthesisofasubstitutedcellulosefromamutantstrainofxanthomonascampestris AT basside biosynthesisofasubstitutedcellulosefromamutantstrainofxanthomonascampestris AT danielsmj biosynthesisofasubstitutedcellulosefromamutantstrainofxanthomonascampestris AT dankertma biosynthesisofasubstitutedcellulosefromamutantstrainofxanthomonascampestris
_version_	1807319007754190848

Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris

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