Matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides
Several commercial sulfated neocarrabiose oligosaccharides were analyzed by matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry (UV-MALDI-TOF-MS). UV-MALDI-TOF-MS was carried out in the linear and reflectron modes and, as routine, in both the positive- and negati...
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todo:paper_00086215_v337_n17_p1553_Fukuyama2023-10-03T14:06:59Z Matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides Fukuyama, Y. Ciancia, M. Nonami, H. Cerezo, A.S. Erra-Balsells, R. Matulewicz, M.C. ESI-TOF-MS kappa-Carrageenan oligosaccharides PSD UV-MALDI-TOF-MS Sulfated neocarrabiose oligosaccharides Sulfated oligosaccharides UV-MALDI-TOF-MS Carboxylic acids Ionization Mass spectrometry Ultraviolet radiation Fragmentation Oligomers gentisic acid harman neocarrabiose 4 sulfate neocarradodecaose 4,4,4,4,4,4 hexasulfate neocarrahexaose 2,4,4,4 tetrasulfate neocarrahexaose 4,4,4 trisulfate neocarraoctaose 4,4,4,4 tetrasulfate neocarratetraose 4 sulfate neocarratetraose 4,4 disulfate oligosaccharide unclassified drug analytic method article carbohydrate analysis chemical bond desorption ionization matrix assisted laser desorption ionization time of flight mass spectrometry priority journal sulfation Carbohydrate Sequence Carrageenan Molecular Sequence Data Oligosaccharides Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfates Ultraviolet Rays Several commercial sulfated neocarrabiose oligosaccharides were analyzed by matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry (UV-MALDI-TOF-MS). UV-MALDI-TOF-MS was carried out in the linear and reflectron modes and, as routine, in both the positive- and negative-ion modes. 2,5-Dihydroxybenzoic acid and nor-harmane were used as matrices. In the positive- and negative-ion modes, with both matrices, peaks corresponding to (M+Na)+ and (M-Na)- ions, respectively, were obtained, with only some signals due to glycosidic linkage cleavages (prompt fragmentation). With 2,5-dihydroxybenzoic acid abundant matrix signals were observed; nor-harmane afforded very few matrix signals in both ion modes, but more desulfation (prompt fragmentation) of the compounds occurred. When the desorption/ionization process was highly efficient, the post-source decay (PSD) fragmentation patterns were also investigated; most of the fragments detected derived from glycosidic linkage cleavages. Electrospray-ionization time-of-flight mass spectrometry (ESI-TOF-MS) in the negative-ion mode confirmed, with the observation of the (M-Na)- and the multiply charged anions, the identity and the purity of the samples. © 2002 Elsevier Science Ltd. All rights reserved. Fil:Ciancia, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00086215_v337_n17_p1553_Fukuyama |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
ESI-TOF-MS kappa-Carrageenan oligosaccharides PSD UV-MALDI-TOF-MS Sulfated neocarrabiose oligosaccharides Sulfated oligosaccharides UV-MALDI-TOF-MS Carboxylic acids Ionization Mass spectrometry Ultraviolet radiation Fragmentation Oligomers gentisic acid harman neocarrabiose 4 sulfate neocarradodecaose 4,4,4,4,4,4 hexasulfate neocarrahexaose 2,4,4,4 tetrasulfate neocarrahexaose 4,4,4 trisulfate neocarraoctaose 4,4,4,4 tetrasulfate neocarratetraose 4 sulfate neocarratetraose 4,4 disulfate oligosaccharide unclassified drug analytic method article carbohydrate analysis chemical bond desorption ionization matrix assisted laser desorption ionization time of flight mass spectrometry priority journal sulfation Carbohydrate Sequence Carrageenan Molecular Sequence Data Oligosaccharides Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfates Ultraviolet Rays |
| spellingShingle |
ESI-TOF-MS kappa-Carrageenan oligosaccharides PSD UV-MALDI-TOF-MS Sulfated neocarrabiose oligosaccharides Sulfated oligosaccharides UV-MALDI-TOF-MS Carboxylic acids Ionization Mass spectrometry Ultraviolet radiation Fragmentation Oligomers gentisic acid harman neocarrabiose 4 sulfate neocarradodecaose 4,4,4,4,4,4 hexasulfate neocarrahexaose 2,4,4,4 tetrasulfate neocarrahexaose 4,4,4 trisulfate neocarraoctaose 4,4,4,4 tetrasulfate neocarratetraose 4 sulfate neocarratetraose 4,4 disulfate oligosaccharide unclassified drug analytic method article carbohydrate analysis chemical bond desorption ionization matrix assisted laser desorption ionization time of flight mass spectrometry priority journal sulfation Carbohydrate Sequence Carrageenan Molecular Sequence Data Oligosaccharides Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfates Ultraviolet Rays Fukuyama, Y. Ciancia, M. Nonami, H. Cerezo, A.S. Erra-Balsells, R. Matulewicz, M.C. Matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides |
| topic_facet |
ESI-TOF-MS kappa-Carrageenan oligosaccharides PSD UV-MALDI-TOF-MS Sulfated neocarrabiose oligosaccharides Sulfated oligosaccharides UV-MALDI-TOF-MS Carboxylic acids Ionization Mass spectrometry Ultraviolet radiation Fragmentation Oligomers gentisic acid harman neocarrabiose 4 sulfate neocarradodecaose 4,4,4,4,4,4 hexasulfate neocarrahexaose 2,4,4,4 tetrasulfate neocarrahexaose 4,4,4 trisulfate neocarraoctaose 4,4,4,4 tetrasulfate neocarratetraose 4 sulfate neocarratetraose 4,4 disulfate oligosaccharide unclassified drug analytic method article carbohydrate analysis chemical bond desorption ionization matrix assisted laser desorption ionization time of flight mass spectrometry priority journal sulfation Carbohydrate Sequence Carrageenan Molecular Sequence Data Oligosaccharides Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfates Ultraviolet Rays |
| description |
Several commercial sulfated neocarrabiose oligosaccharides were analyzed by matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry (UV-MALDI-TOF-MS). UV-MALDI-TOF-MS was carried out in the linear and reflectron modes and, as routine, in both the positive- and negative-ion modes. 2,5-Dihydroxybenzoic acid and nor-harmane were used as matrices. In the positive- and negative-ion modes, with both matrices, peaks corresponding to (M+Na)+ and (M-Na)- ions, respectively, were obtained, with only some signals due to glycosidic linkage cleavages (prompt fragmentation). With 2,5-dihydroxybenzoic acid abundant matrix signals were observed; nor-harmane afforded very few matrix signals in both ion modes, but more desulfation (prompt fragmentation) of the compounds occurred. When the desorption/ionization process was highly efficient, the post-source decay (PSD) fragmentation patterns were also investigated; most of the fragments detected derived from glycosidic linkage cleavages. Electrospray-ionization time-of-flight mass spectrometry (ESI-TOF-MS) in the negative-ion mode confirmed, with the observation of the (M-Na)- and the multiply charged anions, the identity and the purity of the samples. © 2002 Elsevier Science Ltd. All rights reserved. |
| format |
JOUR |
| author |
Fukuyama, Y. Ciancia, M. Nonami, H. Cerezo, A.S. Erra-Balsells, R. Matulewicz, M.C. |
| author_facet |
Fukuyama, Y. Ciancia, M. Nonami, H. Cerezo, A.S. Erra-Balsells, R. Matulewicz, M.C. |
| author_sort |
Fukuyama, Y. |
| title |
Matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides |
| title_short |
Matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides |
| title_full |
Matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides |
| title_fullStr |
Matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides |
| title_full_unstemmed |
Matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides |
| title_sort |
matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides |
| url |
http://hdl.handle.net/20.500.12110/paper_00086215_v337_n17_p1553_Fukuyama |
| work_keys_str_mv |
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