Effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism

Stress activates the synthesis and secretion of catecholamines and adrenal glucocorticoids, increasing their circulating levels. In vivo, hepatic 11β-hydroxysteroid dehydrogenase 1 (HSD1) stimulates the shift of 11-dehydrocorticosterone to corticosterone, enhancing active glucocorticoids at tissue l...

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Autores principales:	Altuna, M.E., Lelli, S.M., San Martín De Viale, L.C., Damasco, M.C.
Formato:	JOUR
Materias:	Carbohydrate metabolism Glucocorticoids Hepatic HSD1 PEPCK Rat Stress 11beta hydroxysteroid dehydrogenase 1 corticosterone dehydrocorticosterone glucocorticoid glycogen hydrochloric acid oxidoreductase phosphoenolpyruvate carboxykinase (GTP) sodium chloride unclassified drug animal experiment article cannula carbohydrate metabolism controlled study enzyme activity enzyme kinetics gluconeogenesis glucose blood level male metabolic acidosis nonhuman priority journal rat stress 11-beta-Hydroxysteroid Dehydrogenase Type 1 11-beta-Hydroxysteroid Dehydrogenases Animals Blood Glucose Carbohydrate Metabolism Corticosterone Cytosol Gluconeogenesis Kinetics Liver Liver Glycogen Male Microsomes, Liver Phosphoenolpyruvate Carboxykinase (ATP) Rats Rats, Sprague-Dawley Stress, Psychological
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00084212_v84_n10_p977_Altuna
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00084212_v84_n10_p977_Altuna2023-10-03T14:06:05Z Effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism Altuna, M.E. Lelli, S.M. San Martín De Viale, L.C. Damasco, M.C. Carbohydrate metabolism Glucocorticoids Hepatic HSD1 PEPCK Rat Stress 11beta hydroxysteroid dehydrogenase 1 corticosterone dehydrocorticosterone glucocorticoid glycogen hydrochloric acid oxidoreductase phosphoenolpyruvate carboxykinase (GTP) sodium chloride unclassified drug animal experiment article cannula carbohydrate metabolism controlled study enzyme activity enzyme kinetics gluconeogenesis glucose blood level male metabolic acidosis nonhuman priority journal rat stress 11-beta-Hydroxysteroid Dehydrogenase Type 1 11-beta-Hydroxysteroid Dehydrogenases Animals Blood Glucose Carbohydrate Metabolism Corticosterone Cytosol Gluconeogenesis Kinetics Liver Liver Glycogen Male Microsomes, Liver Phosphoenolpyruvate Carboxykinase (ATP) Rats Rats, Sprague-Dawley Stress, Psychological Stress activates the synthesis and secretion of catecholamines and adrenal glucocorticoids, increasing their circulating levels. In vivo, hepatic 11β-hydroxysteroid dehydrogenase 1 (HSD1) stimulates the shift of 11-dehydrocorticosterone to corticosterone, enhancing active glucocorticoids at tissue level. We studied the effect of 3 types of stress, 1 induced by bucogastric overload with 200 mmol/L HCl causing metabolic acidosis (HCl), the second induced by bucogastric overload with 0.45% NaCl (NaCl), and the third induced by simulated overload (cannula), on the kinetics of hepatic HSD1 of rats and their influence on the activity of the gluconeogenic enzyme phosphoenolpyruvate carboxykinase, glycemia, and glycogen deposition. Compared with unstressed controls, all types of stress significantly increased HSD1 activity (146% cannula, 130% NaCl, and 253% HCl), phosphoenolpyruvate carboxykinase activity (51% cannula, 48% NaCl, and 86% HCl), and glycemia (29% cannula, 30% NaCl, and 41% HCl), but decreased hepatic glycogen (68% cannula, 68% NaCl, and 78% HCl). Owing to these results, we suggest the following events occur when stress is induced: an increase in hepatic HSD1 activity, augmented active glucocorticoid levels, increased gluconeogenesis, and glycemia. Also involved are the multiple events indirectly related to glucocorticoids, which lead to the depletion of hepatic glycogen deposits, thereby contributing to increased glycemia. This new approach shows that stress increments the activity of hepatic HSD1 and suggests that this enzyme could be involved in the development of the Metabolic Syndrome. © 2006 NRC. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00084212_v84_n10_p977_Altuna
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Carbohydrate metabolism Glucocorticoids Hepatic HSD1 PEPCK Rat Stress 11beta hydroxysteroid dehydrogenase 1 corticosterone dehydrocorticosterone glucocorticoid glycogen hydrochloric acid oxidoreductase phosphoenolpyruvate carboxykinase (GTP) sodium chloride unclassified drug animal experiment article cannula carbohydrate metabolism controlled study enzyme activity enzyme kinetics gluconeogenesis glucose blood level male metabolic acidosis nonhuman priority journal rat stress 11-beta-Hydroxysteroid Dehydrogenase Type 1 11-beta-Hydroxysteroid Dehydrogenases Animals Blood Glucose Carbohydrate Metabolism Corticosterone Cytosol Gluconeogenesis Kinetics Liver Liver Glycogen Male Microsomes, Liver Phosphoenolpyruvate Carboxykinase (ATP) Rats Rats, Sprague-Dawley Stress, Psychological
spellingShingle	Carbohydrate metabolism Glucocorticoids Hepatic HSD1 PEPCK Rat Stress 11beta hydroxysteroid dehydrogenase 1 corticosterone dehydrocorticosterone glucocorticoid glycogen hydrochloric acid oxidoreductase phosphoenolpyruvate carboxykinase (GTP) sodium chloride unclassified drug animal experiment article cannula carbohydrate metabolism controlled study enzyme activity enzyme kinetics gluconeogenesis glucose blood level male metabolic acidosis nonhuman priority journal rat stress 11-beta-Hydroxysteroid Dehydrogenase Type 1 11-beta-Hydroxysteroid Dehydrogenases Animals Blood Glucose Carbohydrate Metabolism Corticosterone Cytosol Gluconeogenesis Kinetics Liver Liver Glycogen Male Microsomes, Liver Phosphoenolpyruvate Carboxykinase (ATP) Rats Rats, Sprague-Dawley Stress, Psychological Altuna, M.E. Lelli, S.M. San Martín De Viale, L.C. Damasco, M.C. Effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism
topic_facet	Carbohydrate metabolism Glucocorticoids Hepatic HSD1 PEPCK Rat Stress 11beta hydroxysteroid dehydrogenase 1 corticosterone dehydrocorticosterone glucocorticoid glycogen hydrochloric acid oxidoreductase phosphoenolpyruvate carboxykinase (GTP) sodium chloride unclassified drug animal experiment article cannula carbohydrate metabolism controlled study enzyme activity enzyme kinetics gluconeogenesis glucose blood level male metabolic acidosis nonhuman priority journal rat stress 11-beta-Hydroxysteroid Dehydrogenase Type 1 11-beta-Hydroxysteroid Dehydrogenases Animals Blood Glucose Carbohydrate Metabolism Corticosterone Cytosol Gluconeogenesis Kinetics Liver Liver Glycogen Male Microsomes, Liver Phosphoenolpyruvate Carboxykinase (ATP) Rats Rats, Sprague-Dawley Stress, Psychological
description	Stress activates the synthesis and secretion of catecholamines and adrenal glucocorticoids, increasing their circulating levels. In vivo, hepatic 11β-hydroxysteroid dehydrogenase 1 (HSD1) stimulates the shift of 11-dehydrocorticosterone to corticosterone, enhancing active glucocorticoids at tissue level. We studied the effect of 3 types of stress, 1 induced by bucogastric overload with 200 mmol/L HCl causing metabolic acidosis (HCl), the second induced by bucogastric overload with 0.45% NaCl (NaCl), and the third induced by simulated overload (cannula), on the kinetics of hepatic HSD1 of rats and their influence on the activity of the gluconeogenic enzyme phosphoenolpyruvate carboxykinase, glycemia, and glycogen deposition. Compared with unstressed controls, all types of stress significantly increased HSD1 activity (146% cannula, 130% NaCl, and 253% HCl), phosphoenolpyruvate carboxykinase activity (51% cannula, 48% NaCl, and 86% HCl), and glycemia (29% cannula, 30% NaCl, and 41% HCl), but decreased hepatic glycogen (68% cannula, 68% NaCl, and 78% HCl). Owing to these results, we suggest the following events occur when stress is induced: an increase in hepatic HSD1 activity, augmented active glucocorticoid levels, increased gluconeogenesis, and glycemia. Also involved are the multiple events indirectly related to glucocorticoids, which lead to the depletion of hepatic glycogen deposits, thereby contributing to increased glycemia. This new approach shows that stress increments the activity of hepatic HSD1 and suggests that this enzyme could be involved in the development of the Metabolic Syndrome. © 2006 NRC.
format	JOUR
author	Altuna, M.E. Lelli, S.M. San Martín De Viale, L.C. Damasco, M.C.
author_facet	Altuna, M.E. Lelli, S.M. San Martín De Viale, L.C. Damasco, M.C.
author_sort	Altuna, M.E.
title	Effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism
title_short	Effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism
title_full	Effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism
title_fullStr	Effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism
title_full_unstemmed	Effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism
title_sort	effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism
url	http://hdl.handle.net/20.500.12110/paper_00084212_v84_n10_p977_Altuna
work_keys_str_mv	AT altuname effectofstressonhepatic11bhydroxysteroiddehydrogenaseactivityanditsinfluenceoncarbohydratemetabolism AT lellism effectofstressonhepatic11bhydroxysteroiddehydrogenaseactivityanditsinfluenceoncarbohydratemetabolism AT sanmartindevialelc effectofstressonhepatic11bhydroxysteroiddehydrogenaseactivityanditsinfluenceoncarbohydratemetabolism AT damascomc effectofstressonhepatic11bhydroxysteroiddehydrogenaseactivityanditsinfluenceoncarbohydratemetabolism
_version_	1782030121994027008

Effect of stress on hepatic 11β-hydroxysteroid dehydrogenase activity and its influence on carbohydrate metabolism

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