Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress
This work studied the effect of stresses produced by simulated gavage or gavage with 200 mmol/L HCl two hours before adrenal extraction, on the activities of the 11β-hydroxysteroid dehydrogenase 1 and 11β-hydroxysteroid dehydrogenase 2 isoforms present in the rat adrenal gland. These activities were...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00084212_v82_n6_p422_Zallocchi |
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todo:paper_00084212_v82_n6_p422_Zallocchi2023-10-03T14:06:05Z Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress Zallocchi, M. Matković, L. Damasco, M.C. Acidosis Adrenal HSD Stress 11beta hydroxysteroid dehydrogenase adrenalin corticosterone dehydrocorticosterone glucocorticoid hydrochloric acid isoenzyme nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate phenylethanolamine n methyltransferase adrenal gland animal experiment animal tissue article controlled study enzyme activation enzyme activity experimental rat feeding homeostasis male measurement microsome nonhuman priority journal protein synthesis rat simulation stress thin layer chromatography This work studied the effect of stresses produced by simulated gavage or gavage with 200 mmol/L HCl two hours before adrenal extraction, on the activities of the 11β-hydroxysteroid dehydrogenase 1 and 11β-hydroxysteroid dehydrogenase 2 isoforms present in the rat adrenal gland. These activities were determined on immediately prepared adrenal microsomes following incubations with 3H-corticosterone and NAD + or NADP+. 11-dehydrocorticosterone was measured as an end-product by TLC, and controls were adrenal microsomes from rats kept under basal (unstressed) conditions. 11β-hydroxysteroid dehydrogenase 1 activity, but not 11β-hydroxysteroid dehydrogenase 2 activity, was increased under both stress-conditions. Homeostatically, the stimulation of 11β- hydroxysteroid dehydrogenase 1 activity would increase the supply of glucocorticoids. These, in turn, would activate the enzyme phenylethanolamine N-methyl transferase, thereby improving the synthesis of epinephrine as part of the stress-response. Fil:Zallocchi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00084212_v82_n6_p422_Zallocchi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acidosis Adrenal HSD Stress 11beta hydroxysteroid dehydrogenase adrenalin corticosterone dehydrocorticosterone glucocorticoid hydrochloric acid isoenzyme nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate phenylethanolamine n methyltransferase adrenal gland animal experiment animal tissue article controlled study enzyme activation enzyme activity experimental rat feeding homeostasis male measurement microsome nonhuman priority journal protein synthesis rat simulation stress thin layer chromatography |
| spellingShingle |
Acidosis Adrenal HSD Stress 11beta hydroxysteroid dehydrogenase adrenalin corticosterone dehydrocorticosterone glucocorticoid hydrochloric acid isoenzyme nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate phenylethanolamine n methyltransferase adrenal gland animal experiment animal tissue article controlled study enzyme activation enzyme activity experimental rat feeding homeostasis male measurement microsome nonhuman priority journal protein synthesis rat simulation stress thin layer chromatography Zallocchi, M. Matković, L. Damasco, M.C. Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress |
| topic_facet |
Acidosis Adrenal HSD Stress 11beta hydroxysteroid dehydrogenase adrenalin corticosterone dehydrocorticosterone glucocorticoid hydrochloric acid isoenzyme nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate phenylethanolamine n methyltransferase adrenal gland animal experiment animal tissue article controlled study enzyme activation enzyme activity experimental rat feeding homeostasis male measurement microsome nonhuman priority journal protein synthesis rat simulation stress thin layer chromatography |
| description |
This work studied the effect of stresses produced by simulated gavage or gavage with 200 mmol/L HCl two hours before adrenal extraction, on the activities of the 11β-hydroxysteroid dehydrogenase 1 and 11β-hydroxysteroid dehydrogenase 2 isoforms present in the rat adrenal gland. These activities were determined on immediately prepared adrenal microsomes following incubations with 3H-corticosterone and NAD + or NADP+. 11-dehydrocorticosterone was measured as an end-product by TLC, and controls were adrenal microsomes from rats kept under basal (unstressed) conditions. 11β-hydroxysteroid dehydrogenase 1 activity, but not 11β-hydroxysteroid dehydrogenase 2 activity, was increased under both stress-conditions. Homeostatically, the stimulation of 11β- hydroxysteroid dehydrogenase 1 activity would increase the supply of glucocorticoids. These, in turn, would activate the enzyme phenylethanolamine N-methyl transferase, thereby improving the synthesis of epinephrine as part of the stress-response. |
| format |
JOUR |
| author |
Zallocchi, M. Matković, L. Damasco, M.C. |
| author_facet |
Zallocchi, M. Matković, L. Damasco, M.C. |
| author_sort |
Zallocchi, M. |
| title |
Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress |
| title_short |
Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress |
| title_full |
Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress |
| title_fullStr |
Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress |
| title_full_unstemmed |
Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress |
| title_sort |
adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress |
| url |
http://hdl.handle.net/20.500.12110/paper_00084212_v82_n6_p422_Zallocchi |
| work_keys_str_mv |
AT zallocchim adrenal11betahydroxysteroiddehydrogenaseactivityinresponsetostress AT matkovicl adrenal11betahydroxysteroiddehydrogenaseactivityinresponsetostress AT damascomc adrenal11betahydroxysteroiddehydrogenaseactivityinresponsetostress |
| _version_ |
1807323454513348608 |