Host gut microorganisms' cues mediate orientation behaviour in the larva of the parasitoid Mallophora ruficauda
The robber fly Mallophora ruficauda is one of the most important apicultural pests in the Pampas region of Argentina. This species is a parasitoid of scarab beetle larvae. Females lay eggs away from the host, and the larvae perform active search behaviour toward Cyclocephala signaticollis third inst...
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todo:paper_00074853_v106_n1_p81_Groba2023-10-03T14:05:37Z Host gut microorganisms' cues mediate orientation behaviour in the larva of the parasitoid Mallophora ruficauda Groba, H.F. Castelo, M.K. Asilidae host location microorganisms parasitoid; Scarabaeidae semiochemicals apiculture bee beetle bioassay chemical cue entomology fly host location host-parasitoid interaction larva microorganism parasitoid pest species protein semiochemical Argentina Pampas Asilidae Mallophora ruficauda Scarabaeidae animal animal behavior association beetle Diptera growth, development and aging host parasite interaction intestine flora larva microbiology orientation parasitology physiology Animals Beetles Behavior, Animal Cues Diptera Gastrointestinal Microbiome Host-Parasite Interactions Larva Orientation The robber fly Mallophora ruficauda is one of the most important apicultural pests in the Pampas region of Argentina. This species is a parasitoid of scarab beetle larvae. Females lay eggs away from the host, and the larvae perform active search behaviour toward Cyclocephala signaticollis third instar larvae, parasitoid's preferred host. This behaviour is mediated by host-related chemical cues produced in hosts' fermentation chamber. Also, C. signaticollis larvae are attracted to fermentation chamber extracts. As scarab larvae have microbe-rich fermentation chamber, it has been suggested that microorganisms could be involved in the production of these semiochemicals. The aims of this work were first to ascertain the presence of microorganisms in the fermentation chamber of C. signaticollis larvae and second to determine the role of microorganisms in the orientation response of parasitoid and host larvae. We found that microorganisms-free C. signaticollis larvae showed deterioration in their development and did not produce the attractive semiochemicals. Therefore, we isolated fermentation chamber microorganisms of host larvae by means of different cultures media, and then, assayed different microorganisms' stimuli by binary choice tests. We were able to isolate microorganisms and determine that M. ruficauda larvae are attracted to semiochemicals from protein degradation in the fermentation chamber. However, C. signaticollis larvae were not attracted to any semiochemicals associated with microorganisms' activity in the fermentation chamber. Although we were unable to elucidate the exact role of gut microorganisms in host behaviour, we discuss their relevance in parasitoid host-seeking behaviour and host conspecific interaction in M. ruficauda-C. signaticollis system. Copyright © Cambridge University Press 2015. Fil:Castelo, M.K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00074853_v106_n1_p81_Groba |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Asilidae host location microorganisms parasitoid; Scarabaeidae semiochemicals apiculture bee beetle bioassay chemical cue entomology fly host location host-parasitoid interaction larva microorganism parasitoid pest species protein semiochemical Argentina Pampas Asilidae Mallophora ruficauda Scarabaeidae animal animal behavior association beetle Diptera growth, development and aging host parasite interaction intestine flora larva microbiology orientation parasitology physiology Animals Beetles Behavior, Animal Cues Diptera Gastrointestinal Microbiome Host-Parasite Interactions Larva Orientation |
| spellingShingle |
Asilidae host location microorganisms parasitoid; Scarabaeidae semiochemicals apiculture bee beetle bioassay chemical cue entomology fly host location host-parasitoid interaction larva microorganism parasitoid pest species protein semiochemical Argentina Pampas Asilidae Mallophora ruficauda Scarabaeidae animal animal behavior association beetle Diptera growth, development and aging host parasite interaction intestine flora larva microbiology orientation parasitology physiology Animals Beetles Behavior, Animal Cues Diptera Gastrointestinal Microbiome Host-Parasite Interactions Larva Orientation Groba, H.F. Castelo, M.K. Host gut microorganisms' cues mediate orientation behaviour in the larva of the parasitoid Mallophora ruficauda |
| topic_facet |
Asilidae host location microorganisms parasitoid; Scarabaeidae semiochemicals apiculture bee beetle bioassay chemical cue entomology fly host location host-parasitoid interaction larva microorganism parasitoid pest species protein semiochemical Argentina Pampas Asilidae Mallophora ruficauda Scarabaeidae animal animal behavior association beetle Diptera growth, development and aging host parasite interaction intestine flora larva microbiology orientation parasitology physiology Animals Beetles Behavior, Animal Cues Diptera Gastrointestinal Microbiome Host-Parasite Interactions Larva Orientation |
| description |
The robber fly Mallophora ruficauda is one of the most important apicultural pests in the Pampas region of Argentina. This species is a parasitoid of scarab beetle larvae. Females lay eggs away from the host, and the larvae perform active search behaviour toward Cyclocephala signaticollis third instar larvae, parasitoid's preferred host. This behaviour is mediated by host-related chemical cues produced in hosts' fermentation chamber. Also, C. signaticollis larvae are attracted to fermentation chamber extracts. As scarab larvae have microbe-rich fermentation chamber, it has been suggested that microorganisms could be involved in the production of these semiochemicals. The aims of this work were first to ascertain the presence of microorganisms in the fermentation chamber of C. signaticollis larvae and second to determine the role of microorganisms in the orientation response of parasitoid and host larvae. We found that microorganisms-free C. signaticollis larvae showed deterioration in their development and did not produce the attractive semiochemicals. Therefore, we isolated fermentation chamber microorganisms of host larvae by means of different cultures media, and then, assayed different microorganisms' stimuli by binary choice tests. We were able to isolate microorganisms and determine that M. ruficauda larvae are attracted to semiochemicals from protein degradation in the fermentation chamber. However, C. signaticollis larvae were not attracted to any semiochemicals associated with microorganisms' activity in the fermentation chamber. Although we were unable to elucidate the exact role of gut microorganisms in host behaviour, we discuss their relevance in parasitoid host-seeking behaviour and host conspecific interaction in M. ruficauda-C. signaticollis system. Copyright © Cambridge University Press 2015. |
| format |
JOUR |
| author |
Groba, H.F. Castelo, M.K. |
| author_facet |
Groba, H.F. Castelo, M.K. |
| author_sort |
Groba, H.F. |
| title |
Host gut microorganisms' cues mediate orientation behaviour in the larva of the parasitoid Mallophora ruficauda |
| title_short |
Host gut microorganisms' cues mediate orientation behaviour in the larva of the parasitoid Mallophora ruficauda |
| title_full |
Host gut microorganisms' cues mediate orientation behaviour in the larva of the parasitoid Mallophora ruficauda |
| title_fullStr |
Host gut microorganisms' cues mediate orientation behaviour in the larva of the parasitoid Mallophora ruficauda |
| title_full_unstemmed |
Host gut microorganisms' cues mediate orientation behaviour in the larva of the parasitoid Mallophora ruficauda |
| title_sort |
host gut microorganisms' cues mediate orientation behaviour in the larva of the parasitoid mallophora ruficauda |
| url |
http://hdl.handle.net/20.500.12110/paper_00074853_v106_n1_p81_Groba |
| work_keys_str_mv |
AT grobahf hostgutmicroorganismscuesmediateorientationbehaviourinthelarvaoftheparasitoidmallophoraruficauda AT castelomk hostgutmicroorganismscuesmediateorientationbehaviourinthelarvaoftheparasitoidmallophoraruficauda |
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