Ontogeny of 5-aminolevulinic dehydratase and porphobilinogen deaminase activities in the yolk sac membrane and liver of chick embryos
1. Chick embryos of 7, 9, 11, 12, 13, 14, 15, 17 and 19 d of embryonic development were examined to determine the activities of 5-aminolevulinic dehydratase (ALA-D, EC 4.2.1.24) and porphobilinogen deaminase (PBG-D, EC 4.3.1.8). 2. Liver and yolk sac membrane ALA-D specific activities showed a maxim...
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todo:paper_00071668_v43_n2_p196_Pauza2023-10-03T14:05:33Z Ontogeny of 5-aminolevulinic dehydratase and porphobilinogen deaminase activities in the yolk sac membrane and liver of chick embryos Pauza, N.L. Sopena De Kracoff, Y.E. Ferramola De Sancovich, A.M. Sancovich, H.A. porphobilinogen deaminase porphobilinogen synthase age animal article chick embryo enzymology growth, development and aging liver metabolism prenatal development spectrophotometry yolk sac Age Factors Animals Chick Embryo Hydroxymethylbilane Synthase Liver Porphobilinogen Synthase Spectrophotometry Yolk Sac 1. Chick embryos of 7, 9, 11, 12, 13, 14, 15, 17 and 19 d of embryonic development were examined to determine the activities of 5-aminolevulinic dehydratase (ALA-D, EC 4.2.1.24) and porphobilinogen deaminase (PBG-D, EC 4.3.1.8). 2. Liver and yolk sac membrane ALA-D specific activities showed a maximum between 12 and 13 d of embryonic development, yolk sac membrane PBG-ase activity a maximum at 9 d and at 7 d in liver. Total activities of ALA-D and PBG-D were not constant during the course of embryonic development but probably related to the changes of intensity of haem synthesis. 3. ALA-D and PBG-ase activities were higher in yolk sac membrane than in liver, showing the importance of the yolk sac membrane as erythropoietic tissue. PBG-D catalysed the rate-limiting reaction of the cytosolic steps in the biosynthetic pathway in both tissues. Fil:Pauza, N.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sopena De Kracoff, Y.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferramola De Sancovich, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sancovich, H.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00071668_v43_n2_p196_Pauza |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
porphobilinogen deaminase porphobilinogen synthase age animal article chick embryo enzymology growth, development and aging liver metabolism prenatal development spectrophotometry yolk sac Age Factors Animals Chick Embryo Hydroxymethylbilane Synthase Liver Porphobilinogen Synthase Spectrophotometry Yolk Sac |
| spellingShingle |
porphobilinogen deaminase porphobilinogen synthase age animal article chick embryo enzymology growth, development and aging liver metabolism prenatal development spectrophotometry yolk sac Age Factors Animals Chick Embryo Hydroxymethylbilane Synthase Liver Porphobilinogen Synthase Spectrophotometry Yolk Sac Pauza, N.L. Sopena De Kracoff, Y.E. Ferramola De Sancovich, A.M. Sancovich, H.A. Ontogeny of 5-aminolevulinic dehydratase and porphobilinogen deaminase activities in the yolk sac membrane and liver of chick embryos |
| topic_facet |
porphobilinogen deaminase porphobilinogen synthase age animal article chick embryo enzymology growth, development and aging liver metabolism prenatal development spectrophotometry yolk sac Age Factors Animals Chick Embryo Hydroxymethylbilane Synthase Liver Porphobilinogen Synthase Spectrophotometry Yolk Sac |
| description |
1. Chick embryos of 7, 9, 11, 12, 13, 14, 15, 17 and 19 d of embryonic development were examined to determine the activities of 5-aminolevulinic dehydratase (ALA-D, EC 4.2.1.24) and porphobilinogen deaminase (PBG-D, EC 4.3.1.8). 2. Liver and yolk sac membrane ALA-D specific activities showed a maximum between 12 and 13 d of embryonic development, yolk sac membrane PBG-ase activity a maximum at 9 d and at 7 d in liver. Total activities of ALA-D and PBG-D were not constant during the course of embryonic development but probably related to the changes of intensity of haem synthesis. 3. ALA-D and PBG-ase activities were higher in yolk sac membrane than in liver, showing the importance of the yolk sac membrane as erythropoietic tissue. PBG-D catalysed the rate-limiting reaction of the cytosolic steps in the biosynthetic pathway in both tissues. |
| format |
JOUR |
| author |
Pauza, N.L. Sopena De Kracoff, Y.E. Ferramola De Sancovich, A.M. Sancovich, H.A. |
| author_facet |
Pauza, N.L. Sopena De Kracoff, Y.E. Ferramola De Sancovich, A.M. Sancovich, H.A. |
| author_sort |
Pauza, N.L. |
| title |
Ontogeny of 5-aminolevulinic dehydratase and porphobilinogen deaminase activities in the yolk sac membrane and liver of chick embryos |
| title_short |
Ontogeny of 5-aminolevulinic dehydratase and porphobilinogen deaminase activities in the yolk sac membrane and liver of chick embryos |
| title_full |
Ontogeny of 5-aminolevulinic dehydratase and porphobilinogen deaminase activities in the yolk sac membrane and liver of chick embryos |
| title_fullStr |
Ontogeny of 5-aminolevulinic dehydratase and porphobilinogen deaminase activities in the yolk sac membrane and liver of chick embryos |
| title_full_unstemmed |
Ontogeny of 5-aminolevulinic dehydratase and porphobilinogen deaminase activities in the yolk sac membrane and liver of chick embryos |
| title_sort |
ontogeny of 5-aminolevulinic dehydratase and porphobilinogen deaminase activities in the yolk sac membrane and liver of chick embryos |
| url |
http://hdl.handle.net/20.500.12110/paper_00071668_v43_n2_p196_Pauza |
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