Glucocorticoid effects on Fos immunoreactivity and NADPH-diaphorase histochemical staining following spinal cord injury
Glucocorticoids (GC) provide neuroprotection and early recovery after spinal cord injury (SCI). While several mechanisms were proposed to account for these effects, limited information exists regarding GC actions in sensory areas of the spinal cord. Presently, we studied the time course of Fos expre...
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todo:paper_00068993_v912_n2_p144_Gonzalez2023-10-03T14:05:24Z Glucocorticoid effects on Fos immunoreactivity and NADPH-diaphorase histochemical staining following spinal cord injury González, S. Labombarda, F. Gonzalez Deniselle, M.C. Saravia, F.E. Roig, P. De Nicola, A.F. Dexamethasone Fos Glococorticoid NADPH-diaphorase Neuroprotection Spinal cord injury dexamethasone glucocorticoid nitric oxide protein fos reduced nicotinamide adenine dinucleotide phosphate dehydrogenase adrenergic system animal experiment animal model animal tissue article cell activation cell count cell population computer analysis controlled study dose time effect relation down regulation image analysis immunocompetent cell immunohistochemistry immunoreactivity male nerve potential nociception nonhuman pain priority journal protein expression protein induction rat spinal cord dorsal horn spinal cord injury spinal cord transsection thoracic spinal cord time vertebral canal Animals Cell Count Dexamethasone Dose-Response Relationship, Drug Down-Regulation Drug Administration Schedule Glucocorticoids Immunohistochemistry Male NADPH Dehydrogenase Nitric Oxide Pain Proto-Oncogene Proteins c-fos Rats Rats, Sprague-Dawley Spinal Cord Injuries Substantia Gelatinosa Time Factors Up-Regulation Glucocorticoids (GC) provide neuroprotection and early recovery after spinal cord injury (SCI). While several mechanisms were proposed to account for these effects, limited information exists regarding GC actions in sensory areas of the spinal cord. Presently, we studied the time course of Fos expression, and reduced nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemical staining to monitor neuronal responses to SCI with or without GC treatment. Rats with sham-operation or transection at the thoracic level (T7-T8) received vehicle or 5 mg/kg of the GC dexamethasone (DEX) at 5 min post-lesion and were sacrificed 2 or 4 h after surgery. Another group of SCI rats received vehicle or intensive DEX treatment (5 min, 6 h, 18 h and 46 h post-lesion) and were sacrificed 48 h after surgery. The number of NADPH-d positive neurons or Fos immunoreactive nuclei was studied by computer-assisted image analysis in superficial dorsal horn (Laminae I-III) and central canal area (Lamina X) below the lesion. While constitutive Fos immunoreactive nuclei were sparse in controls, SCI increased Fos expression at 2 and 4 h after injury. DEX treatment significantly enhanced the number of Fos positive nuclei in Laminae I-III by 4 h after transection, although the response was not maintained by intensive steroid treatment when tested at 48 h after SCI. NADPH-d positive neurons in Laminae I-III increased at 2 and 4 h after SCI while a delayed increased was found in central canal area (Lamina X). DEX treatment decreased NADPH-d positive neurons to sham-operated levels at all time points examined. Thus, while GC stimulation of Fos suggests activation of neurons involved in sympathetic outflow and/or pain, down-regulation of NADPH-d indicates attenuation of nociceptive outflow, considering the role of enzyme-derived nitric oxide in pain-related mechanisms. Differential hormonal effects on these molecules agree with their localization in different cell populations. © 2001 Elsevier Science B.V. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00068993_v912_n2_p144_Gonzalez |
| institution |
Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Dexamethasone Fos Glococorticoid NADPH-diaphorase Neuroprotection Spinal cord injury dexamethasone glucocorticoid nitric oxide protein fos reduced nicotinamide adenine dinucleotide phosphate dehydrogenase adrenergic system animal experiment animal model animal tissue article cell activation cell count cell population computer analysis controlled study dose time effect relation down regulation image analysis immunocompetent cell immunohistochemistry immunoreactivity male nerve potential nociception nonhuman pain priority journal protein expression protein induction rat spinal cord dorsal horn spinal cord injury spinal cord transsection thoracic spinal cord time vertebral canal Animals Cell Count Dexamethasone Dose-Response Relationship, Drug Down-Regulation Drug Administration Schedule Glucocorticoids Immunohistochemistry Male NADPH Dehydrogenase Nitric Oxide Pain Proto-Oncogene Proteins c-fos Rats Rats, Sprague-Dawley Spinal Cord Injuries Substantia Gelatinosa Time Factors Up-Regulation |
| spellingShingle |
Dexamethasone Fos Glococorticoid NADPH-diaphorase Neuroprotection Spinal cord injury dexamethasone glucocorticoid nitric oxide protein fos reduced nicotinamide adenine dinucleotide phosphate dehydrogenase adrenergic system animal experiment animal model animal tissue article cell activation cell count cell population computer analysis controlled study dose time effect relation down regulation image analysis immunocompetent cell immunohistochemistry immunoreactivity male nerve potential nociception nonhuman pain priority journal protein expression protein induction rat spinal cord dorsal horn spinal cord injury spinal cord transsection thoracic spinal cord time vertebral canal Animals Cell Count Dexamethasone Dose-Response Relationship, Drug Down-Regulation Drug Administration Schedule Glucocorticoids Immunohistochemistry Male NADPH Dehydrogenase Nitric Oxide Pain Proto-Oncogene Proteins c-fos Rats Rats, Sprague-Dawley Spinal Cord Injuries Substantia Gelatinosa Time Factors Up-Regulation González, S. Labombarda, F. Gonzalez Deniselle, M.C. Saravia, F.E. Roig, P. De Nicola, A.F. Glucocorticoid effects on Fos immunoreactivity and NADPH-diaphorase histochemical staining following spinal cord injury |
| topic_facet |
Dexamethasone Fos Glococorticoid NADPH-diaphorase Neuroprotection Spinal cord injury dexamethasone glucocorticoid nitric oxide protein fos reduced nicotinamide adenine dinucleotide phosphate dehydrogenase adrenergic system animal experiment animal model animal tissue article cell activation cell count cell population computer analysis controlled study dose time effect relation down regulation image analysis immunocompetent cell immunohistochemistry immunoreactivity male nerve potential nociception nonhuman pain priority journal protein expression protein induction rat spinal cord dorsal horn spinal cord injury spinal cord transsection thoracic spinal cord time vertebral canal Animals Cell Count Dexamethasone Dose-Response Relationship, Drug Down-Regulation Drug Administration Schedule Glucocorticoids Immunohistochemistry Male NADPH Dehydrogenase Nitric Oxide Pain Proto-Oncogene Proteins c-fos Rats Rats, Sprague-Dawley Spinal Cord Injuries Substantia Gelatinosa Time Factors Up-Regulation |
| description |
Glucocorticoids (GC) provide neuroprotection and early recovery after spinal cord injury (SCI). While several mechanisms were proposed to account for these effects, limited information exists regarding GC actions in sensory areas of the spinal cord. Presently, we studied the time course of Fos expression, and reduced nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemical staining to monitor neuronal responses to SCI with or without GC treatment. Rats with sham-operation or transection at the thoracic level (T7-T8) received vehicle or 5 mg/kg of the GC dexamethasone (DEX) at 5 min post-lesion and were sacrificed 2 or 4 h after surgery. Another group of SCI rats received vehicle or intensive DEX treatment (5 min, 6 h, 18 h and 46 h post-lesion) and were sacrificed 48 h after surgery. The number of NADPH-d positive neurons or Fos immunoreactive nuclei was studied by computer-assisted image analysis in superficial dorsal horn (Laminae I-III) and central canal area (Lamina X) below the lesion. While constitutive Fos immunoreactive nuclei were sparse in controls, SCI increased Fos expression at 2 and 4 h after injury. DEX treatment significantly enhanced the number of Fos positive nuclei in Laminae I-III by 4 h after transection, although the response was not maintained by intensive steroid treatment when tested at 48 h after SCI. NADPH-d positive neurons in Laminae I-III increased at 2 and 4 h after SCI while a delayed increased was found in central canal area (Lamina X). DEX treatment decreased NADPH-d positive neurons to sham-operated levels at all time points examined. Thus, while GC stimulation of Fos suggests activation of neurons involved in sympathetic outflow and/or pain, down-regulation of NADPH-d indicates attenuation of nociceptive outflow, considering the role of enzyme-derived nitric oxide in pain-related mechanisms. Differential hormonal effects on these molecules agree with their localization in different cell populations. © 2001 Elsevier Science B.V. All rights reserved. |
| format |
JOUR |
| author |
González, S. Labombarda, F. Gonzalez Deniselle, M.C. Saravia, F.E. Roig, P. De Nicola, A.F. |
| author_facet |
González, S. Labombarda, F. Gonzalez Deniselle, M.C. Saravia, F.E. Roig, P. De Nicola, A.F. |
| author_sort |
González, S. |
| title |
Glucocorticoid effects on Fos immunoreactivity and NADPH-diaphorase histochemical staining following spinal cord injury |
| title_short |
Glucocorticoid effects on Fos immunoreactivity and NADPH-diaphorase histochemical staining following spinal cord injury |
| title_full |
Glucocorticoid effects on Fos immunoreactivity and NADPH-diaphorase histochemical staining following spinal cord injury |
| title_fullStr |
Glucocorticoid effects on Fos immunoreactivity and NADPH-diaphorase histochemical staining following spinal cord injury |
| title_full_unstemmed |
Glucocorticoid effects on Fos immunoreactivity and NADPH-diaphorase histochemical staining following spinal cord injury |
| title_sort |
glucocorticoid effects on fos immunoreactivity and nadph-diaphorase histochemical staining following spinal cord injury |
| url |
http://hdl.handle.net/20.500.12110/paper_00068993_v912_n2_p144_Gonzalez |
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