Glucocorticoid regulation of mRNA encoding (Na + K)ATPase α3 and β1 subunits in rat brain measured by in situ hybridization

The effect of glucocorticoids on (Na + K)ATPase mRNA synthesis was studied in 19 brain areas of adrenalectomized (ADX) rats untreated or receiving dexamethasone (DEX). For in situ hybridization, we employed a [35S]oligonucleotide probe for the α3-subunit isoform, and a [3H]cDNA coding for the β1-sub...

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Autores principales: Grillo, C., Piroli, G., González, S.L., Angulo, J., McEwen, B.S., De Nicola, A.F.
Formato: JOUR
Materias:
(Na + K)ATPase
Adrenalectomy
Dexamethasone
In situ hybridization
adenosine triphosphatase (potassium sodium)
dexamethasone
glucocorticoid
adrenalectomy
animal experiment
animal tissue
article
brain region
controlled study
enzyme activity
in situ hybridization
male
nonhuman
oral drug administration
priority journal
rat
rna synthesis
Adrenal Glands
Animal
Base Sequence
Brain
Genetic Code
In Situ Hybridization
Male
Molecular Sequence Data
Na(+)-K(+)-Exchanging ATPase
Peptide Fragments
Rats
Rats, Sprague-Dawley
RNA, Messenger
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00068993_v657_n1-2_p83_Grillo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_00068993_v657_n1-2_p83_Grillo2023-10-03T14:05:22Z Glucocorticoid regulation of mRNA encoding (Na + K)ATPase α3 and β1 subunits in rat brain measured by in situ hybridization Grillo, C. Piroli, G. González, S.L. Angulo, J. McEwen, B.S. De Nicola, A.F. (Na + K)ATPase Adrenalectomy Dexamethasone In situ hybridization adenosine triphosphatase (potassium sodium) dexamethasone glucocorticoid adrenalectomy animal experiment animal tissue article brain region controlled study enzyme activity in situ hybridization male nonhuman oral drug administration priority journal rat rna synthesis Adrenal Glands Adrenalectomy Animal Base Sequence Brain Dexamethasone Genetic Code In Situ Hybridization Male Molecular Sequence Data Na(+)-K(+)-Exchanging ATPase Peptide Fragments Rats Rats, Sprague-Dawley RNA, Messenger The effect of glucocorticoids on (Na + K)ATPase mRNA synthesis was studied in 19 brain areas of adrenalectomized (ADX) rats untreated or receiving dexamethasone (DEX). For in situ hybridization, we employed a [35S]oligonucleotide probe for the α3-subunit isoform, and a [3H]cDNA coding for the β1-subunit of the enzyme. Mean levels of grain density for the α3 subunit mRNA of DEX-treated rats were significantly higher by a 't' test in medial septum, amygdala lateralis (AL) and medialis (AME), gyrus dentatus, CA4 hippocampal area, substantia nigra and periventricular gray, compared to untreated rats. For the β1-subunit, mean levels after DEX were significantly higher in AL and lateral preoptic area. In addition, the Kolmogorov-Smirnov test applied to frequency histograms of neuronal densities indicated a coordinate increase in α3 and β1-subunit mRNA expression for the CA2 subfield and preoptic area medialis (POA MED). We conclude that (1) glucocorticoids are positive modulators of (Na + K)ATPase mRNA; (2) analysis of frequency histograms suggests that glucocorticoids promote in a few regions (AL, POA MED, CA2 subfield) a coordinate increase in the biosynthesis of the α3 and β1-subunit mRNA. In 11 other areas stimulation occurs for one subunit mRNA only, whereas 5 areas were insensitive to glucocorticoid effects on this enzyme. © 1994. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00068993_v657_n1-2_p83_Grillo
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic (Na + K)ATPase
Adrenalectomy
Dexamethasone
In situ hybridization
adenosine triphosphatase (potassium sodium)
dexamethasone
glucocorticoid
adrenalectomy
animal experiment
animal tissue
article
brain region
controlled study
enzyme activity
in situ hybridization
male
nonhuman
oral drug administration
priority journal
rat
rna synthesis
Adrenal Glands
Adrenalectomy
Animal
Base Sequence
Brain
Dexamethasone
Genetic Code
In Situ Hybridization
Male
Molecular Sequence Data
Na(+)-K(+)-Exchanging ATPase
Peptide Fragments
Rats
Rats, Sprague-Dawley
RNA, Messenger
spellingShingle (Na + K)ATPase
Adrenalectomy
Dexamethasone
In situ hybridization
adenosine triphosphatase (potassium sodium)
dexamethasone
glucocorticoid
adrenalectomy
animal experiment
animal tissue
article
brain region
controlled study
enzyme activity
in situ hybridization
male
nonhuman
oral drug administration
priority journal
rat
rna synthesis
Adrenal Glands
Adrenalectomy
Animal
Base Sequence
Brain
Dexamethasone
Genetic Code
In Situ Hybridization
Male
Molecular Sequence Data
Na(+)-K(+)-Exchanging ATPase
Peptide Fragments
Rats
Rats, Sprague-Dawley
RNA, Messenger
Grillo, C.
Piroli, G.
González, S.L.
Angulo, J.
McEwen, B.S.
De Nicola, A.F.
Glucocorticoid regulation of mRNA encoding (Na + K)ATPase α3 and β1 subunits in rat brain measured by in situ hybridization
topic_facet (Na + K)ATPase
Adrenalectomy
Dexamethasone
In situ hybridization
adenosine triphosphatase (potassium sodium)
dexamethasone
glucocorticoid
adrenalectomy
animal experiment
animal tissue
article
brain region
controlled study
enzyme activity
in situ hybridization
male
nonhuman
oral drug administration
priority journal
rat
rna synthesis
Adrenal Glands
Adrenalectomy
Animal
Base Sequence
Brain
Dexamethasone
Genetic Code
In Situ Hybridization
Male
Molecular Sequence Data
Na(+)-K(+)-Exchanging ATPase
Peptide Fragments
Rats
Rats, Sprague-Dawley
RNA, Messenger
description The effect of glucocorticoids on (Na + K)ATPase mRNA synthesis was studied in 19 brain areas of adrenalectomized (ADX) rats untreated or receiving dexamethasone (DEX). For in situ hybridization, we employed a [35S]oligonucleotide probe for the α3-subunit isoform, and a [3H]cDNA coding for the β1-subunit of the enzyme. Mean levels of grain density for the α3 subunit mRNA of DEX-treated rats were significantly higher by a 't' test in medial septum, amygdala lateralis (AL) and medialis (AME), gyrus dentatus, CA4 hippocampal area, substantia nigra and periventricular gray, compared to untreated rats. For the β1-subunit, mean levels after DEX were significantly higher in AL and lateral preoptic area. In addition, the Kolmogorov-Smirnov test applied to frequency histograms of neuronal densities indicated a coordinate increase in α3 and β1-subunit mRNA expression for the CA2 subfield and preoptic area medialis (POA MED). We conclude that (1) glucocorticoids are positive modulators of (Na + K)ATPase mRNA; (2) analysis of frequency histograms suggests that glucocorticoids promote in a few regions (AL, POA MED, CA2 subfield) a coordinate increase in the biosynthesis of the α3 and β1-subunit mRNA. In 11 other areas stimulation occurs for one subunit mRNA only, whereas 5 areas were insensitive to glucocorticoid effects on this enzyme. © 1994.
format JOUR
author Grillo, C.
Piroli, G.
González, S.L.
Angulo, J.
McEwen, B.S.
De Nicola, A.F.
author_facet Grillo, C.
Piroli, G.
González, S.L.
Angulo, J.
McEwen, B.S.
De Nicola, A.F.
author_sort Grillo, C.
title Glucocorticoid regulation of mRNA encoding (Na + K)ATPase α3 and β1 subunits in rat brain measured by in situ hybridization
title_short Glucocorticoid regulation of mRNA encoding (Na + K)ATPase α3 and β1 subunits in rat brain measured by in situ hybridization
title_full Glucocorticoid regulation of mRNA encoding (Na + K)ATPase α3 and β1 subunits in rat brain measured by in situ hybridization
title_fullStr Glucocorticoid regulation of mRNA encoding (Na + K)ATPase α3 and β1 subunits in rat brain measured by in situ hybridization
title_full_unstemmed Glucocorticoid regulation of mRNA encoding (Na + K)ATPase α3 and β1 subunits in rat brain measured by in situ hybridization
title_sort glucocorticoid regulation of mrna encoding (na + k)atpase α3 and β1 subunits in rat brain measured by in situ hybridization
url http://hdl.handle.net/20.500.12110/paper_00068993_v657_n1-2_p83_Grillo
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AT anguloj glucocorticoidregulationofmrnaencodingnakatpasea3andb1subunitsinratbrainmeasuredbyinsituhybridization
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